Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24270 and RR42_RS24275 are separated by 108 nucleotides RR42_RS24275 and RR42_RS24280 overlap by 4 nucleotides RR42_RS24280 and RR42_RS24285 are separated by 1 nucleotides
RR42_RS24270: RR42_RS24270 - hypothetical protein, at 772,514 to 773,482
_RS24270
RR42_RS24275: RR42_RS24275 - hypothetical protein, at 773,591 to 774,478
_RS24275
RR42_RS24280: RR42_RS24280 - gentisate 1,2-dioxygenase, at 774,475 to 775,524
_RS24280
RR42_RS24285: RR42_RS24285 - 2-polyprenyl-6-methoxyphenol hydroxylase, at 775,526 to 777,196
_RS24285
Position (kb) 774
775
776 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 773.504 kb on - strand at 773.592 kb on + strand at 773.593 kb on - strand at 773.595 kb on + strand at 773.595 kb on + strand at 773.595 kb on + strand at 773.596 kb on - strand at 773.675 kb on - strand at 773.834 kb on + strand, within RR42_RS24275 at 773.834 kb on + strand, within RR42_RS24275 at 773.834 kb on + strand, within RR42_RS24275 at 773.835 kb on - strand, within RR42_RS24275 at 774.000 kb on + strand, within RR42_RS24275 at 774.000 kb on + strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.401 kb on + strand at 774.402 kb on - strand at 774.420 kb on + strand at 774.420 kb on + strand at 774.420 kb on + strand at 774.421 kb on - strand at 774.421 kb on - strand at 774.493 kb on - strand at 774.668 kb on + strand, within RR42_RS24280 at 774.669 kb on - strand, within RR42_RS24280 at 774.671 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.803 kb on + strand, within RR42_RS24280 at 774.863 kb on + strand, within RR42_RS24280 at 775.257 kb on + strand, within RR42_RS24280 at 775.257 kb on + strand, within RR42_RS24280 at 775.458 kb on - strand at 775.524 kb on + strand at 775.524 kb on + strand at 775.524 kb on + strand at 775.525 kb on - strand at 775.525 kb on - strand at 775.545 kb on + strand at 775.661 kb on + strand at 775.661 kb on + strand at 775.683 kb on + strand at 775.683 kb on + strand at 775.684 kb on - strand at 775.684 kb on - strand at 775.684 kb on - strand at 775.684 kb on - strand at 775.799 kb on + strand, within RR42_RS24285 at 775.799 kb on + strand, within RR42_RS24285 at 775.800 kb on - strand, within RR42_RS24285 at 775.800 kb on - strand, within RR42_RS24285 at 775.803 kb on + strand, within RR42_RS24285 at 775.803 kb on + strand, within RR42_RS24285 at 775.823 kb on + strand, within RR42_RS24285 at 775.953 kb on + strand, within RR42_RS24285 at 775.960 kb on - strand, within RR42_RS24285 at 776.008 kb on + strand, within RR42_RS24285 at 776.008 kb on + strand, within RR42_RS24285 at 776.008 kb on + strand, within RR42_RS24285 at 776.009 kb on - strand, within RR42_RS24285 at 776.009 kb on - strand, within RR42_RS24285 at 776.260 kb on + strand, within RR42_RS24285 at 776.261 kb on - strand, within RR42_RS24285 at 776.462 kb on + strand, within RR42_RS24285 at 776.462 kb on + strand, within RR42_RS24285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 773,504 - +0.5 773,592 + +0.5 773,593 - -2.4 773,595 + +0.9 773,595 + +0.0 773,595 + -2.4 773,596 - -0.3 773,675 - +0.8 773,834 + RR42_RS24275 0.27 -0.7 773,834 + RR42_RS24275 0.27 -0.0 773,834 + RR42_RS24275 0.27 -1.9 773,835 - RR42_RS24275 0.27 -2.4 774,000 + RR42_RS24275 0.46 -2.4 774,000 + RR42_RS24275 0.46 +0.3 774,243 - RR42_RS24275 0.73 +0.3 774,243 - RR42_RS24275 0.73 -1.4 774,243 - RR42_RS24275 0.73 -0.4 774,243 - RR42_RS24275 0.73 +0.3 774,401 + -0.2 774,402 - +0.1 774,420 + -2.5 774,420 + +3.3 774,420 + +0.4 774,421 - -1.5 774,421 - -0.1 774,493 - -0.2 774,668 + RR42_RS24280 0.18 +0.4 774,669 - RR42_RS24280 0.18 -0.7 774,671 + RR42_RS24280 0.19 +1.6 774,803 + RR42_RS24280 0.31 -0.5 774,803 + RR42_RS24280 0.31 +1.1 774,803 + RR42_RS24280 0.31 +0.2 774,803 + RR42_RS24280 0.31 -0.4 774,863 + RR42_RS24280 0.37 +0.5 775,257 + RR42_RS24280 0.74 -0.2 775,257 + RR42_RS24280 0.74 +0.5 775,458 - +0.4 775,524 + -1.4 775,524 + -1.6 775,524 + +0.0 775,525 - -0.6 775,525 - -1.3 775,545 + +0.6 775,661 + -0.8 775,661 + +0.1 775,683 + +0.8 775,683 + -1.7 775,684 - -1.0 775,684 - -0.2 775,684 - +0.1 775,684 - +0.6 775,799 + RR42_RS24285 0.16 +0.3 775,799 + RR42_RS24285 0.16 -0.1 775,800 - RR42_RS24285 0.16 -0.3 775,800 - RR42_RS24285 0.16 -0.1 775,803 + RR42_RS24285 0.17 -0.6 775,803 + RR42_RS24285 0.17 -0.8 775,823 + RR42_RS24285 0.18 -0.0 775,953 + RR42_RS24285 0.26 -0.0 775,960 - RR42_RS24285 0.26 +0.7 776,008 + RR42_RS24285 0.29 +0.5 776,008 + RR42_RS24285 0.29 +0.4 776,008 + RR42_RS24285 0.29 -0.0 776,009 - RR42_RS24285 0.29 +0.1 776,009 - RR42_RS24285 0.29 +0.7 776,260 + RR42_RS24285 0.44 -0.5 776,261 - RR42_RS24285 0.44 -1.2 776,462 + RR42_RS24285 0.56 -0.4 776,462 + RR42_RS24285 0.56 +0.9
Or see this region's nucleotide sequence