Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23605 and RR42_RS23610 are separated by 5 nucleotides RR42_RS23610 and RR42_RS23615 overlap by 4 nucleotides
RR42_RS23605: RR42_RS23605 - CDP-glucose 4,6-dehydratase, at 620,962 to 622,020
_RS23605
RR42_RS23610: RR42_RS23610 - glucose-1-phosphate cytidylyltransferase, at 622,026 to 622,799
_RS23610
RR42_RS23615: RR42_RS23615 - hypothetical protein, at 622,796 to 625,021
_RS23615
Position (kb) 622
623 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 621.036 kb on - strand at 621.314 kb on + strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.618 kb on - strand, within RR42_RS23605 at 621.911 kb on + strand, within RR42_RS23605 at 621.947 kb on - strand at 621.947 kb on - strand at 622.007 kb on + strand at 622.007 kb on + strand at 622.008 kb on - strand at 622.008 kb on - strand at 622.015 kb on + strand at 622.016 kb on - strand at 622.016 kb on - strand at 622.016 kb on - strand at 622.016 kb on - strand at 622.302 kb on - strand, within RR42_RS23610 at 622.318 kb on + strand, within RR42_RS23610 at 622.319 kb on - strand, within RR42_RS23610 at 622.319 kb on - strand, within RR42_RS23610 at 622.424 kb on + strand, within RR42_RS23610 at 622.425 kb on - strand, within RR42_RS23610 at 622.425 kb on - strand, within RR42_RS23610 at 622.453 kb on + strand, within RR42_RS23610 at 622.589 kb on + strand, within RR42_RS23610 at 622.590 kb on - strand, within RR42_RS23610 at 622.601 kb on - strand, within RR42_RS23610 at 622.613 kb on - strand, within RR42_RS23610 at 622.636 kb on + strand, within RR42_RS23610 at 622.679 kb on - strand, within RR42_RS23610 at 622.974 kb on + strand at 623.003 kb on + strand at 623.003 kb on + strand at 623.023 kb on - strand, within RR42_RS23615 at 623.394 kb on + strand, within RR42_RS23615 at 623.395 kb on - strand, within RR42_RS23615 at 623.395 kb on - strand, within RR42_RS23615 at 623.395 kb on - strand, within RR42_RS23615 at 623.432 kb on + strand, within RR42_RS23615 at 623.552 kb on - strand, within RR42_RS23615 at 623.568 kb on - strand, within RR42_RS23615 at 623.586 kb on + strand, within RR42_RS23615
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 621,036 - -0.7 621,314 + RR42_RS23605 0.33 +2.9 621,555 - RR42_RS23605 0.56 +1.0 621,555 - RR42_RS23605 0.56 -0.7 621,555 - RR42_RS23605 0.56 -0.7 621,618 - RR42_RS23605 0.62 +1.3 621,911 + RR42_RS23605 0.90 -0.6 621,947 - +1.1 621,947 - +0.7 622,007 + +0.9 622,007 + +0.0 622,008 - +0.2 622,008 - +0.3 622,015 + -0.8 622,016 - +0.6 622,016 - -0.2 622,016 - +1.1 622,016 - +1.3 622,302 - RR42_RS23610 0.36 +0.2 622,318 + RR42_RS23610 0.38 +0.1 622,319 - RR42_RS23610 0.38 +1.4 622,319 - RR42_RS23610 0.38 -1.2 622,424 + RR42_RS23610 0.51 +0.2 622,425 - RR42_RS23610 0.52 +1.3 622,425 - RR42_RS23610 0.52 -0.6 622,453 + RR42_RS23610 0.55 -0.3 622,589 + RR42_RS23610 0.73 -1.1 622,590 - RR42_RS23610 0.73 +1.8 622,601 - RR42_RS23610 0.74 +0.5 622,613 - RR42_RS23610 0.76 +0.7 622,636 + RR42_RS23610 0.79 +1.0 622,679 - RR42_RS23610 0.84 -0.5 622,974 + +0.1 623,003 + +0.3 623,003 + -1.3 623,023 - RR42_RS23615 0.10 -2.2 623,394 + RR42_RS23615 0.27 +0.3 623,395 - RR42_RS23615 0.27 +0.9 623,395 - RR42_RS23615 0.27 +1.8 623,395 - RR42_RS23615 0.27 -0.1 623,432 + RR42_RS23615 0.29 +0.3 623,552 - RR42_RS23615 0.34 -1.3 623,568 - RR42_RS23615 0.35 +0.6 623,586 + RR42_RS23615 0.35 -1.6
Or see this region's nucleotide sequence