Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23035 and RR42_RS23040 are separated by 398 nucleotides RR42_RS23040 and RR42_RS23045 are separated by 165 nucleotides RR42_RS23045 and RR42_RS23050 are separated by 60 nucleotides
RR42_RS23035: RR42_RS23035 - sporulation protein, at 502,881 to 503,831
_RS23035
RR42_RS23040: RR42_RS23040 - hypothetical protein, at 504,230 to 504,514
_RS23040
RR42_RS23045: RR42_RS23045 - LysR family transcriptional regulator, at 504,680 to 505,585
_RS23045
RR42_RS23050: RR42_RS23050 - 4-oxalomesaconate tautomerase, at 505,646 to 506,725
_RS23050
Position (kb) 504
505
506 Strain fitness (log2 ratio) -2
-1
0
1
2 at 503.771 kb on + strand at 503.900 kb on + strand at 503.906 kb on + strand at 503.907 kb on - strand at 503.932 kb on + strand at 503.957 kb on + strand at 503.958 kb on - strand at 503.995 kb on - strand at 504.017 kb on + strand at 504.034 kb on + strand at 504.035 kb on - strand at 504.035 kb on - strand at 504.035 kb on - strand at 504.084 kb on - strand at 504.084 kb on - strand at 504.085 kb on + strand at 504.085 kb on + strand at 504.085 kb on + strand at 504.085 kb on + strand at 504.086 kb on - strand at 504.087 kb on + strand at 504.088 kb on - strand at 504.088 kb on - strand at 504.175 kb on + strand at 504.176 kb on - strand at 504.214 kb on - strand at 504.222 kb on + strand at 504.223 kb on - strand at 504.239 kb on - strand at 504.473 kb on + strand, within RR42_RS23040 at 504.478 kb on + strand, within RR42_RS23040 at 504.536 kb on + strand at 504.536 kb on + strand at 504.536 kb on + strand at 504.539 kb on + strand at 504.540 kb on - strand at 504.540 kb on - strand at 504.543 kb on + strand at 504.543 kb on + strand at 504.543 kb on + strand at 504.547 kb on + strand at 504.547 kb on + strand at 504.547 kb on + strand at 504.548 kb on - strand at 504.548 kb on - strand at 504.586 kb on - strand at 504.586 kb on - strand at 504.586 kb on - strand at 504.635 kb on - strand at 504.682 kb on - strand at 505.028 kb on + strand, within RR42_RS23045 at 505.028 kb on + strand, within RR42_RS23045 at 505.200 kb on - strand, within RR42_RS23045 at 505.222 kb on - strand, within RR42_RS23045 at 505.261 kb on - strand, within RR42_RS23045 at 505.261 kb on - strand, within RR42_RS23045 at 505.264 kb on - strand, within RR42_RS23045 at 505.264 kb on - strand, within RR42_RS23045 at 505.613 kb on + strand at 505.613 kb on + strand at 505.614 kb on - strand at 505.614 kb on - strand at 505.628 kb on - strand at 505.669 kb on - strand at 505.749 kb on + strand at 505.749 kb on + strand at 505.750 kb on - strand at 505.866 kb on + strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.908 kb on + strand, within RR42_RS23050 at 506.023 kb on + strand, within RR42_RS23050 at 506.075 kb on + strand, within RR42_RS23050 at 506.076 kb on - strand, within RR42_RS23050 at 506.086 kb on + strand, within RR42_RS23050 at 506.221 kb on + strand, within RR42_RS23050 at 506.513 kb on + strand, within RR42_RS23050 at 506.514 kb on - strand, within RR42_RS23050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 503,771 + -0.8 503,900 + -0.1 503,906 + +1.3 503,907 - -0.4 503,932 + -0.6 503,957 + +0.6 503,958 - +0.5 503,995 - +0.4 504,017 + -1.0 504,034 + +1.0 504,035 - -0.6 504,035 - +0.7 504,035 - +0.4 504,084 - -0.2 504,084 - +0.5 504,085 + -0.1 504,085 + +0.2 504,085 + +0.1 504,085 + +0.4 504,086 - +0.2 504,087 + +0.3 504,088 - +0.1 504,088 - +0.7 504,175 + -0.1 504,176 - +1.5 504,214 - -0.2 504,222 + +0.3 504,223 - -0.1 504,239 - +2.1 504,473 + RR42_RS23040 0.85 -2.1 504,478 + RR42_RS23040 0.87 -0.6 504,536 + -0.4 504,536 + +1.2 504,536 + +1.0 504,539 + -0.0 504,540 - -1.2 504,540 - +0.5 504,543 + -1.1 504,543 + +0.3 504,543 + +1.3 504,547 + -2.0 504,547 + +0.1 504,547 + -1.3 504,548 - -0.1 504,548 - -0.3 504,586 - +0.1 504,586 - +1.1 504,586 - -0.4 504,635 - +0.7 504,682 - +0.6 505,028 + RR42_RS23045 0.38 -1.3 505,028 + RR42_RS23045 0.38 -0.1 505,200 - RR42_RS23045 0.57 -0.4 505,222 - RR42_RS23045 0.60 -1.2 505,261 - RR42_RS23045 0.64 +0.4 505,261 - RR42_RS23045 0.64 +1.3 505,264 - RR42_RS23045 0.64 +0.2 505,264 - RR42_RS23045 0.64 +0.7 505,613 + -0.3 505,613 + +0.5 505,614 - -0.0 505,614 - -1.1 505,628 - +1.5 505,669 - +0.4 505,749 + -0.2 505,749 + -0.6 505,750 - +1.3 505,866 + RR42_RS23050 0.20 -0.2 505,867 - RR42_RS23050 0.20 +0.8 505,867 - RR42_RS23050 0.20 +0.3 505,867 - RR42_RS23050 0.20 +0.3 505,867 - RR42_RS23050 0.20 +0.1 505,867 - RR42_RS23050 0.20 +0.8 505,908 + RR42_RS23050 0.24 +0.3 506,023 + RR42_RS23050 0.35 -0.4 506,075 + RR42_RS23050 0.40 +0.2 506,076 - RR42_RS23050 0.40 -0.1 506,086 + RR42_RS23050 0.41 -0.3 506,221 + RR42_RS23050 0.53 -1.5 506,513 + RR42_RS23050 0.80 -0.6 506,514 - RR42_RS23050 0.80 +0.1
Or see this region's nucleotide sequence