Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23005 and RR42_RS23010 are separated by 289 nucleotides RR42_RS23010 and RR42_RS23015 are separated by 163 nucleotides RR42_RS23015 and RR42_RS23020 are separated by 4 nucleotides
RR42_RS23005: RR42_RS23005 - hypothetical protein, at 496,800 to 497,303
_RS23005
RR42_RS23010: RR42_RS23010 - hypothetical protein, at 497,593 to 498,051
_RS23010
RR42_RS23015: RR42_RS23015 - hypothetical protein, at 498,215 to 498,757
_RS23015
RR42_RS23020: RR42_RS23020 - ABC transporter substrate-binding protein, at 498,762 to 499,742
_RS23020
Position (kb)
498
499 Strain fitness (log2 ratio)
-2
-1
0
1 at 497.238 kb on + strand, within RR42_RS23005 at 497.402 kb on - strand at 497.508 kb on + strand at 497.508 kb on + strand at 497.652 kb on - strand, within RR42_RS23010 at 497.652 kb on - strand, within RR42_RS23010 at 497.652 kb on - strand, within RR42_RS23010 at 497.904 kb on - strand, within RR42_RS23010 at 497.904 kb on - strand, within RR42_RS23010 at 497.904 kb on - strand, within RR42_RS23010 at 497.904 kb on - strand, within RR42_RS23010 at 497.937 kb on - strand, within RR42_RS23010 at 498.151 kb on + strand at 498.151 kb on + strand at 498.151 kb on + strand at 498.365 kb on + strand, within RR42_RS23015 at 498.371 kb on + strand, within RR42_RS23015 at 498.372 kb on - strand, within RR42_RS23015 at 498.372 kb on - strand, within RR42_RS23015 at 498.415 kb on + strand, within RR42_RS23015 at 498.415 kb on + strand, within RR42_RS23015 at 498.499 kb on + strand, within RR42_RS23015 at 498.499 kb on + strand, within RR42_RS23015 at 498.500 kb on - strand, within RR42_RS23015 at 498.500 kb on - strand, within RR42_RS23015 at 498.510 kb on + strand, within RR42_RS23015 at 498.510 kb on + strand, within RR42_RS23015 at 498.510 kb on + strand, within RR42_RS23015 at 498.510 kb on + strand, within RR42_RS23015 at 498.511 kb on - strand, within RR42_RS23015 at 498.511 kb on - strand, within RR42_RS23015 at 498.511 kb on - strand, within RR42_RS23015 at 498.511 kb on - strand, within RR42_RS23015 at 498.511 kb on - strand, within RR42_RS23015 at 498.555 kb on - strand, within RR42_RS23015 at 498.555 kb on - strand, within RR42_RS23015 at 498.586 kb on - strand, within RR42_RS23015 at 498.699 kb on - strand, within RR42_RS23015 at 498.979 kb on + strand, within RR42_RS23020 at 498.979 kb on + strand, within RR42_RS23020 at 498.980 kb on - strand, within RR42_RS23020 at 498.997 kb on + strand, within RR42_RS23020 at 499.180 kb on + strand, within RR42_RS23020 at 499.180 kb on + strand, within RR42_RS23020 at 499.181 kb on - strand, within RR42_RS23020 at 499.267 kb on + strand, within RR42_RS23020 at 499.267 kb on + strand, within RR42_RS23020 at 499.267 kb on + strand, within RR42_RS23020 at 499.267 kb on + strand, within RR42_RS23020 at 499.267 kb on + strand, within RR42_RS23020 at 499.268 kb on - strand, within RR42_RS23020 at 499.268 kb on - strand, within RR42_RS23020 at 499.272 kb on + strand, within RR42_RS23020 at 499.273 kb on - strand, within RR42_RS23020 at 499.273 kb on - strand, within RR42_RS23020 at 499.364 kb on - strand, within RR42_RS23020 at 499.412 kb on + strand, within RR42_RS23020 at 499.413 kb on - strand, within RR42_RS23020 at 499.413 kb on - strand, within RR42_RS23020 at 499.413 kb on - strand, within RR42_RS23020 at 499.511 kb on + strand, within RR42_RS23020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 497,238 + RR42_RS23005 0.87 +1.3 497,402 - +0.9 497,508 + +0.9 497,508 + +0.0 497,652 - RR42_RS23010 0.13 +0.1 497,652 - RR42_RS23010 0.13 -0.6 497,652 - RR42_RS23010 0.13 -0.0 497,904 - RR42_RS23010 0.68 +0.3 497,904 - RR42_RS23010 0.68 +1.0 497,904 - RR42_RS23010 0.68 -0.1 497,904 - RR42_RS23010 0.68 -0.3 497,937 - RR42_RS23010 0.75 +0.3 498,151 + -0.2 498,151 + +0.7 498,151 + -1.9 498,365 + RR42_RS23015 0.28 +1.0 498,371 + RR42_RS23015 0.29 -0.5 498,372 - RR42_RS23015 0.29 -0.0 498,372 - RR42_RS23015 0.29 +0.2 498,415 + RR42_RS23015 0.37 -1.3 498,415 + RR42_RS23015 0.37 -0.3 498,499 + RR42_RS23015 0.52 -0.1 498,499 + RR42_RS23015 0.52 +0.5 498,500 - RR42_RS23015 0.52 +0.3 498,500 - RR42_RS23015 0.52 +0.3 498,510 + RR42_RS23015 0.54 +0.7 498,510 + RR42_RS23015 0.54 -1.4 498,510 + RR42_RS23015 0.54 +0.3 498,510 + RR42_RS23015 0.54 -0.2 498,511 - RR42_RS23015 0.55 +0.9 498,511 - RR42_RS23015 0.55 +1.0 498,511 - RR42_RS23015 0.55 +1.2 498,511 - RR42_RS23015 0.55 +0.3 498,511 - RR42_RS23015 0.55 -0.7 498,555 - RR42_RS23015 0.63 -0.3 498,555 - RR42_RS23015 0.63 +1.6 498,586 - RR42_RS23015 0.68 +0.6 498,699 - RR42_RS23015 0.89 -0.3 498,979 + RR42_RS23020 0.22 -0.3 498,979 + RR42_RS23020 0.22 -1.9 498,980 - RR42_RS23020 0.22 +0.9 498,997 + RR42_RS23020 0.24 -0.7 499,180 + RR42_RS23020 0.43 +1.3 499,180 + RR42_RS23020 0.43 -0.3 499,181 - RR42_RS23020 0.43 +0.5 499,267 + RR42_RS23020 0.51 -1.3 499,267 + RR42_RS23020 0.51 +0.2 499,267 + RR42_RS23020 0.51 -1.3 499,267 + RR42_RS23020 0.51 -0.3 499,267 + RR42_RS23020 0.51 -0.7 499,268 - RR42_RS23020 0.52 +0.7 499,268 - RR42_RS23020 0.52 -0.7 499,272 + RR42_RS23020 0.52 -0.4 499,273 - RR42_RS23020 0.52 -0.5 499,273 - RR42_RS23020 0.52 +0.1 499,364 - RR42_RS23020 0.61 +0.6 499,412 + RR42_RS23020 0.66 +0.8 499,413 - RR42_RS23020 0.66 -0.5 499,413 - RR42_RS23020 0.66 +0.6 499,413 - RR42_RS23020 0.66 +0.1 499,511 + RR42_RS23020 0.76 +1.1
Or see this region's nucleotide sequence