Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22650 and RR42_RS22655 are separated by 75 nucleotides RR42_RS22655 and RR42_RS22660 are separated by 56 nucleotides
RR42_RS22650: RR42_RS22650 - ABC transporter substrate-binding protein, at 415,591 to 416,580
_RS22650
RR42_RS22655: RR42_RS22655 - mandelate racemase, at 416,656 to 417,813
_RS22655
RR42_RS22660: RR42_RS22660 - hypothetical protein, at 417,870 to 418,847
_RS22660
Position (kb) 416
417
418 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 415.687 kb on + strand at 415.688 kb on - strand at 415.870 kb on + strand, within RR42_RS22650 at 415.871 kb on - strand, within RR42_RS22650 at 415.912 kb on + strand, within RR42_RS22650 at 415.913 kb on - strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.159 kb on - strand, within RR42_RS22650 at 416.277 kb on - strand, within RR42_RS22650 at 416.302 kb on + strand, within RR42_RS22650 at 416.386 kb on - strand, within RR42_RS22650 at 416.386 kb on - strand, within RR42_RS22650 at 416.531 kb on + strand at 416.579 kb on - strand at 416.725 kb on + strand at 416.854 kb on + strand, within RR42_RS22655 at 416.854 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.110 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.460 kb on - strand, within RR42_RS22655 at 417.856 kb on + strand at 417.856 kb on + strand at 417.893 kb on - strand at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.173 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.252 kb on - strand, within RR42_RS22660 at 418.308 kb on + strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.417 kb on - strand, within RR42_RS22660 at 418.425 kb on + strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.583 kb on + strand, within RR42_RS22660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 415,687 + -0.1 415,688 - -1.5 415,870 + RR42_RS22650 0.28 -1.3 415,871 - RR42_RS22650 0.28 -2.5 415,912 + RR42_RS22650 0.32 +0.1 415,913 - RR42_RS22650 0.33 -0.0 415,921 + RR42_RS22650 0.33 +0.4 415,921 + RR42_RS22650 0.33 -0.6 415,922 - RR42_RS22650 0.33 +0.6 415,922 - RR42_RS22650 0.33 +0.4 415,922 - RR42_RS22650 0.33 -1.5 415,922 - RR42_RS22650 0.33 +0.1 416,065 + RR42_RS22650 0.48 -0.6 416,065 + RR42_RS22650 0.48 -0.2 416,158 + RR42_RS22650 0.57 +1.7 416,158 + RR42_RS22650 0.57 -0.4 416,159 - RR42_RS22650 0.57 -0.2 416,277 - RR42_RS22650 0.69 +1.6 416,302 + RR42_RS22650 0.72 +0.9 416,386 - RR42_RS22650 0.80 +0.6 416,386 - RR42_RS22650 0.80 -3.0 416,531 + +0.2 416,579 - -0.8 416,725 + -0.2 416,854 + RR42_RS22655 0.17 +0.2 416,854 + RR42_RS22655 0.17 -0.1 417,109 + +1.3 417,109 + RR42_RS22655 0.39 -0.1 417,109 + RR42_RS22655 0.39 -0.5 417,109 + RR42_RS22655 0.39 -2.2 417,109 + RR42_RS22655 0.39 -1.3 417,110 - RR42_RS22655 0.39 -0.5 417,136 - RR42_RS22655 0.41 +0.3 417,136 - RR42_RS22655 0.41 +0.5 417,460 - RR42_RS22655 0.69 -0.3 417,856 + +0.6 417,856 + +1.0 417,893 - -0.5 418,137 + RR42_RS22660 0.27 -0.5 418,137 + RR42_RS22660 0.27 -0.9 418,137 + RR42_RS22660 0.27 -0.3 418,173 + RR42_RS22660 0.31 -1.0 418,185 + RR42_RS22660 0.32 -0.3 418,185 + RR42_RS22660 0.32 -0.6 418,186 - RR42_RS22660 0.32 -3.5 418,186 - RR42_RS22660 0.32 +1.3 418,251 + RR42_RS22660 0.39 -0.1 418,251 + RR42_RS22660 0.39 +0.9 418,252 - RR42_RS22660 0.39 -1.5 418,308 + RR42_RS22660 0.45 -0.5 418,309 - RR42_RS22660 0.45 +0.4 418,309 - RR42_RS22660 0.45 -1.2 418,339 - RR42_RS22660 0.48 +0.5 418,339 - RR42_RS22660 0.48 -3.7 418,416 + RR42_RS22660 0.56 +0.4 418,416 + RR42_RS22660 0.56 -0.4 418,416 + RR42_RS22660 0.56 +0.1 418,416 + RR42_RS22660 0.56 +0.3 418,417 - RR42_RS22660 0.56 -0.5 418,425 + RR42_RS22660 0.57 -0.2 418,426 - RR42_RS22660 0.57 -0.3 418,426 - RR42_RS22660 0.57 +0.3 418,510 - RR42_RS22660 0.65 +1.2 418,510 - RR42_RS22660 0.65 -0.9 418,510 - RR42_RS22660 0.65 -0.8 418,510 - RR42_RS22660 0.65 +0.3 418,583 + RR42_RS22660 0.73 +0.8
Or see this region's nucleotide sequence