Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22645 and RR42_RS22650 are separated by 230 nucleotides RR42_RS22650 and RR42_RS22655 are separated by 75 nucleotides
RR42_RS22645: RR42_RS22645 - GntR family transcriptional regulator, at 414,308 to 415,360
_RS22645
RR42_RS22650: RR42_RS22650 - ABC transporter substrate-binding protein, at 415,591 to 416,580
_RS22650
RR42_RS22655: RR42_RS22655 - mandelate racemase, at 416,656 to 417,813
_RS22655
Position (kb) 415
416
417 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 414.782 kb on - strand, within RR42_RS22645 at 414.782 kb on - strand, within RR42_RS22645 at 414.782 kb on - strand, within RR42_RS22645 at 414.869 kb on + strand, within RR42_RS22645 at 415.388 kb on + strand at 415.527 kb on + strand at 415.527 kb on + strand at 415.687 kb on + strand at 415.688 kb on - strand at 415.870 kb on + strand, within RR42_RS22650 at 415.871 kb on - strand, within RR42_RS22650 at 415.912 kb on + strand, within RR42_RS22650 at 415.913 kb on - strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.159 kb on - strand, within RR42_RS22650 at 416.277 kb on - strand, within RR42_RS22650 at 416.302 kb on + strand, within RR42_RS22650 at 416.386 kb on - strand, within RR42_RS22650 at 416.386 kb on - strand, within RR42_RS22650 at 416.531 kb on + strand at 416.579 kb on - strand at 416.725 kb on + strand at 416.854 kb on + strand, within RR42_RS22655 at 416.854 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.110 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.460 kb on - strand, within RR42_RS22655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 414,782 - RR42_RS22645 0.45 +1.2 414,782 - RR42_RS22645 0.45 +1.9 414,782 - RR42_RS22645 0.45 -2.0 414,869 + RR42_RS22645 0.53 -0.4 415,388 + +0.1 415,527 + +0.9 415,527 + +0.7 415,687 + -0.1 415,688 - -1.5 415,870 + RR42_RS22650 0.28 -1.3 415,871 - RR42_RS22650 0.28 -2.5 415,912 + RR42_RS22650 0.32 +0.1 415,913 - RR42_RS22650 0.33 -0.0 415,921 + RR42_RS22650 0.33 +0.4 415,921 + RR42_RS22650 0.33 -0.6 415,922 - RR42_RS22650 0.33 +0.6 415,922 - RR42_RS22650 0.33 +0.4 415,922 - RR42_RS22650 0.33 -1.5 415,922 - RR42_RS22650 0.33 +0.1 416,065 + RR42_RS22650 0.48 -0.6 416,065 + RR42_RS22650 0.48 -0.2 416,158 + RR42_RS22650 0.57 +1.7 416,158 + RR42_RS22650 0.57 -0.4 416,159 - RR42_RS22650 0.57 -0.2 416,277 - RR42_RS22650 0.69 +1.6 416,302 + RR42_RS22650 0.72 +0.9 416,386 - RR42_RS22650 0.80 +0.6 416,386 - RR42_RS22650 0.80 -3.0 416,531 + +0.2 416,579 - -0.8 416,725 + -0.2 416,854 + RR42_RS22655 0.17 +0.2 416,854 + RR42_RS22655 0.17 -0.1 417,109 + +1.3 417,109 + RR42_RS22655 0.39 -0.1 417,109 + RR42_RS22655 0.39 -0.5 417,109 + RR42_RS22655 0.39 -2.2 417,109 + RR42_RS22655 0.39 -1.3 417,110 - RR42_RS22655 0.39 -0.5 417,136 - RR42_RS22655 0.41 +0.3 417,136 - RR42_RS22655 0.41 +0.5 417,460 - RR42_RS22655 0.69 -0.3
Or see this region's nucleotide sequence