Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22480 and RR42_RS22485 are separated by 54 nucleotides RR42_RS22485 and RR42_RS22490 are separated by 304 nucleotides
RR42_RS22480: RR42_RS22480 - FAD-dependent oxidoreductase, at 379,846 to 381,579
_RS22480
RR42_RS22485: RR42_RS22485 - MBL fold metallo-hydrolase, at 381,634 to 382,593
_RS22485
RR42_RS22490: RR42_RS22490 - IclR family transcriptional regulator, at 382,898 to 383,725
_RS22490
Position (kb) 381
382
383 Strain fitness (log2 ratio) -2
-1
0
1
2 at 380.698 kb on - strand, within RR42_RS22480 at 380.698 kb on - strand, within RR42_RS22480 at 380.698 kb on - strand, within RR42_RS22480 at 380.699 kb on + strand, within RR42_RS22480 at 381.047 kb on + strand, within RR42_RS22480 at 381.048 kb on - strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.195 kb on - strand, within RR42_RS22480 at 381.195 kb on - strand, within RR42_RS22480 at 381.195 kb on - strand, within RR42_RS22480 at 381.197 kb on + strand, within RR42_RS22480 at 381.197 kb on + strand, within RR42_RS22480 at 381.198 kb on - strand, within RR42_RS22480 at 381.260 kb on + strand, within RR42_RS22480 at 381.260 kb on + strand, within RR42_RS22480 at 381.261 kb on - strand, within RR42_RS22480 at 381.617 kb on + strand at 381.752 kb on + strand, within RR42_RS22485 at 381.752 kb on + strand, within RR42_RS22485 at 381.752 kb on + strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.882 kb on - strand, within RR42_RS22485 at 382.028 kb on + strand, within RR42_RS22485 at 382.029 kb on - strand, within RR42_RS22485 at 382.029 kb on - strand, within RR42_RS22485 at 382.277 kb on + strand, within RR42_RS22485 at 382.278 kb on - strand, within RR42_RS22485 at 382.322 kb on + strand, within RR42_RS22485 at 382.323 kb on - strand, within RR42_RS22485 at 382.356 kb on - strand, within RR42_RS22485 at 382.373 kb on + strand, within RR42_RS22485 at 382.374 kb on - strand, within RR42_RS22485 at 382.512 kb on - strand at 382.517 kb on + strand at 382.517 kb on + strand at 382.518 kb on - strand at 382.695 kb on + strand at 382.696 kb on - strand at 382.696 kb on - strand at 382.696 kb on - strand at 382.696 kb on - strand at 382.696 kb on - strand at 382.697 kb on + strand at 382.697 kb on + strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.728 kb on + strand at 382.798 kb on - strand at 382.869 kb on + strand at 382.869 kb on + strand at 382.869 kb on + strand at 382.870 kb on - strand at 382.870 kb on - strand at 382.870 kb on - strand at 382.872 kb on + strand at 382.872 kb on + strand at 382.872 kb on + strand at 382.873 kb on - strand at 382.873 kb on - strand at 382.873 kb on - strand at 382.873 kb on - strand at 383.093 kb on + strand, within RR42_RS22490 at 383.093 kb on + strand, within RR42_RS22490 at 383.093 kb on + strand, within RR42_RS22490 at 383.093 kb on + strand, within RR42_RS22490 at 383.093 kb on + strand, within RR42_RS22490 at 383.094 kb on - strand, within RR42_RS22490 at 383.189 kb on + strand, within RR42_RS22490 at 383.189 kb on + strand, within RR42_RS22490 at 383.190 kb on - strand, within RR42_RS22490 at 383.190 kb on - strand, within RR42_RS22490 at 383.310 kb on - strand, within RR42_RS22490
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 380,698 - RR42_RS22480 0.49 -1.3 380,698 - RR42_RS22480 0.49 -1.3 380,698 - RR42_RS22480 0.49 -0.2 380,699 + RR42_RS22480 0.49 +2.3 381,047 + RR42_RS22480 0.69 +1.3 381,048 - RR42_RS22480 0.69 -0.0 381,194 + RR42_RS22480 0.78 +0.5 381,194 + RR42_RS22480 0.78 -0.5 381,194 + RR42_RS22480 0.78 +1.3 381,194 + RR42_RS22480 0.78 -0.8 381,194 + RR42_RS22480 0.78 -0.3 381,194 + RR42_RS22480 0.78 -0.1 381,195 - RR42_RS22480 0.78 +0.3 381,195 - RR42_RS22480 0.78 -0.1 381,195 - RR42_RS22480 0.78 +0.1 381,197 + RR42_RS22480 0.78 -0.6 381,197 + RR42_RS22480 0.78 -1.7 381,198 - RR42_RS22480 0.78 -0.2 381,260 + RR42_RS22480 0.82 -1.6 381,260 + RR42_RS22480 0.82 -0.3 381,261 - RR42_RS22480 0.82 +0.4 381,617 + +1.0 381,752 + RR42_RS22485 0.12 +0.3 381,752 + RR42_RS22485 0.12 -0.6 381,752 + RR42_RS22485 0.12 -0.2 381,753 - RR42_RS22485 0.12 +0.1 381,753 - RR42_RS22485 0.12 +0.6 381,753 - RR42_RS22485 0.12 +1.3 381,753 - RR42_RS22485 0.12 +1.2 381,753 - RR42_RS22485 0.12 +0.1 381,882 - RR42_RS22485 0.26 +0.3 382,028 + RR42_RS22485 0.41 -0.7 382,029 - RR42_RS22485 0.41 -0.4 382,029 - RR42_RS22485 0.41 +0.5 382,277 + RR42_RS22485 0.67 +0.3 382,278 - RR42_RS22485 0.67 +0.4 382,322 + RR42_RS22485 0.72 +1.3 382,323 - RR42_RS22485 0.72 +0.3 382,356 - RR42_RS22485 0.75 +0.1 382,373 + RR42_RS22485 0.77 +0.3 382,374 - RR42_RS22485 0.77 +0.3 382,512 - +0.6 382,517 + +0.8 382,517 + -1.3 382,518 - +0.2 382,695 + -0.7 382,696 - -0.9 382,696 - -1.8 382,696 - +1.1 382,696 - +0.1 382,696 - -0.4 382,697 + +0.3 382,697 + +1.3 382,698 - +1.2 382,698 - -0.9 382,698 - +0.2 382,698 - -0.6 382,698 - +0.0 382,698 - +0.7 382,728 + -1.3 382,798 - +1.3 382,869 + +1.3 382,869 + -0.1 382,869 + -0.6 382,870 - -1.1 382,870 - +0.2 382,870 - -0.3 382,872 + +0.1 382,872 + -0.0 382,872 + +0.6 382,873 - +0.7 382,873 - -0.9 382,873 - -0.2 382,873 - +0.4 383,093 + RR42_RS22490 0.24 +0.3 383,093 + RR42_RS22490 0.24 -0.4 383,093 + RR42_RS22490 0.24 -1.3 383,093 + RR42_RS22490 0.24 -1.1 383,093 + RR42_RS22490 0.24 +0.4 383,094 - RR42_RS22490 0.24 +1.9 383,189 + RR42_RS22490 0.35 +0.0 383,189 + RR42_RS22490 0.35 +1.2 383,190 - RR42_RS22490 0.35 -0.5 383,190 - RR42_RS22490 0.35 -0.3 383,310 - RR42_RS22490 0.50 +0.0
Or see this region's nucleotide sequence