Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22470 and RR42_RS22475 are separated by 101 nucleotides RR42_RS22475 and RR42_RS22480 are separated by 35 nucleotides RR42_RS22480 and RR42_RS22485 are separated by 54 nucleotides
RR42_RS22470: RR42_RS22470 - MFS transporter, at 378,168 to 379,493
_RS22470
RR42_RS22475: RR42_RS22475 - hypothetical protein, at 379,595 to 379,810
_RS22475
RR42_RS22480: RR42_RS22480 - FAD-dependent oxidoreductase, at 379,846 to 381,579
_RS22480
RR42_RS22485: RR42_RS22485 - MBL fold metallo-hydrolase, at 381,634 to 382,593
_RS22485
Position (kb) 379
380
381
382 Strain fitness (log2 ratio) -1
0
1
2 at 378.934 kb on + strand, within RR42_RS22470 at 378.935 kb on - strand, within RR42_RS22470 at 379.073 kb on - strand, within RR42_RS22470 at 379.073 kb on - strand, within RR42_RS22470 at 379.073 kb on - strand, within RR42_RS22470 at 379.133 kb on - strand, within RR42_RS22470 at 379.133 kb on - strand, within RR42_RS22470 at 379.274 kb on - strand, within RR42_RS22470 at 379.274 kb on - strand, within RR42_RS22470 at 379.274 kb on - strand, within RR42_RS22470 at 379.274 kb on - strand, within RR42_RS22470 at 379.298 kb on - strand, within RR42_RS22470 at 379.376 kb on + strand at 379.377 kb on - strand at 379.381 kb on + strand at 379.381 kb on + strand at 379.382 kb on - strand at 379.382 kb on - strand at 379.382 kb on - strand at 379.998 kb on - strand at 380.022 kb on - strand, within RR42_RS22480 at 380.203 kb on + strand, within RR42_RS22480 at 380.204 kb on - strand, within RR42_RS22480 at 380.356 kb on - strand, within RR42_RS22480 at 380.356 kb on - strand, within RR42_RS22480 at 380.493 kb on - strand, within RR42_RS22480 at 380.510 kb on + strand, within RR42_RS22480 at 380.511 kb on - strand, within RR42_RS22480 at 380.511 kb on - strand, within RR42_RS22480 at 380.698 kb on - strand, within RR42_RS22480 at 380.698 kb on - strand, within RR42_RS22480 at 380.698 kb on - strand, within RR42_RS22480 at 380.699 kb on + strand, within RR42_RS22480 at 381.047 kb on + strand, within RR42_RS22480 at 381.048 kb on - strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.194 kb on + strand, within RR42_RS22480 at 381.195 kb on - strand, within RR42_RS22480 at 381.195 kb on - strand, within RR42_RS22480 at 381.195 kb on - strand, within RR42_RS22480 at 381.197 kb on + strand, within RR42_RS22480 at 381.197 kb on + strand, within RR42_RS22480 at 381.198 kb on - strand, within RR42_RS22480 at 381.260 kb on + strand, within RR42_RS22480 at 381.260 kb on + strand, within RR42_RS22480 at 381.261 kb on - strand, within RR42_RS22480 at 381.617 kb on + strand at 381.752 kb on + strand, within RR42_RS22485 at 381.752 kb on + strand, within RR42_RS22485 at 381.752 kb on + strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.753 kb on - strand, within RR42_RS22485 at 381.882 kb on - strand, within RR42_RS22485 at 382.028 kb on + strand, within RR42_RS22485 at 382.029 kb on - strand, within RR42_RS22485 at 382.029 kb on - strand, within RR42_RS22485 at 382.277 kb on + strand, within RR42_RS22485 at 382.278 kb on - strand, within RR42_RS22485 at 382.322 kb on + strand, within RR42_RS22485 at 382.323 kb on - strand, within RR42_RS22485 at 382.356 kb on - strand, within RR42_RS22485 at 382.373 kb on + strand, within RR42_RS22485 at 382.374 kb on - strand, within RR42_RS22485 at 382.512 kb on - strand at 382.517 kb on + strand at 382.517 kb on + strand at 382.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 378,934 + RR42_RS22470 0.58 +1.2 378,935 - RR42_RS22470 0.58 -1.0 379,073 - RR42_RS22470 0.68 +0.6 379,073 - RR42_RS22470 0.68 -1.3 379,073 - RR42_RS22470 0.68 +0.4 379,133 - RR42_RS22470 0.73 +0.4 379,133 - RR42_RS22470 0.73 +1.3 379,274 - RR42_RS22470 0.83 +0.9 379,274 - RR42_RS22470 0.83 +0.9 379,274 - RR42_RS22470 0.83 -1.2 379,274 - RR42_RS22470 0.83 -0.1 379,298 - RR42_RS22470 0.85 -1.1 379,376 + +2.3 379,377 - -0.8 379,381 + +0.1 379,381 + -0.6 379,382 - +0.7 379,382 - +0.8 379,382 - +0.3 379,998 - +0.3 380,022 - RR42_RS22480 0.10 -1.6 380,203 + RR42_RS22480 0.21 +0.6 380,204 - RR42_RS22480 0.21 +0.8 380,356 - RR42_RS22480 0.29 +1.3 380,356 - RR42_RS22480 0.29 -0.5 380,493 - RR42_RS22480 0.37 -0.4 380,510 + RR42_RS22480 0.38 -0.9 380,511 - RR42_RS22480 0.38 -1.0 380,511 - RR42_RS22480 0.38 -1.3 380,698 - RR42_RS22480 0.49 -1.3 380,698 - RR42_RS22480 0.49 -1.3 380,698 - RR42_RS22480 0.49 -0.2 380,699 + RR42_RS22480 0.49 +2.3 381,047 + RR42_RS22480 0.69 +1.3 381,048 - RR42_RS22480 0.69 -0.0 381,194 + RR42_RS22480 0.78 +0.5 381,194 + RR42_RS22480 0.78 -0.5 381,194 + RR42_RS22480 0.78 +1.3 381,194 + RR42_RS22480 0.78 -0.8 381,194 + RR42_RS22480 0.78 -0.3 381,194 + RR42_RS22480 0.78 -0.1 381,195 - RR42_RS22480 0.78 +0.3 381,195 - RR42_RS22480 0.78 -0.1 381,195 - RR42_RS22480 0.78 +0.1 381,197 + RR42_RS22480 0.78 -0.6 381,197 + RR42_RS22480 0.78 -1.7 381,198 - RR42_RS22480 0.78 -0.2 381,260 + RR42_RS22480 0.82 -1.6 381,260 + RR42_RS22480 0.82 -0.3 381,261 - RR42_RS22480 0.82 +0.4 381,617 + +1.0 381,752 + RR42_RS22485 0.12 +0.3 381,752 + RR42_RS22485 0.12 -0.6 381,752 + RR42_RS22485 0.12 -0.2 381,753 - RR42_RS22485 0.12 +0.1 381,753 - RR42_RS22485 0.12 +0.6 381,753 - RR42_RS22485 0.12 +1.3 381,753 - RR42_RS22485 0.12 +1.2 381,753 - RR42_RS22485 0.12 +0.1 381,882 - RR42_RS22485 0.26 +0.3 382,028 + RR42_RS22485 0.41 -0.7 382,029 - RR42_RS22485 0.41 -0.4 382,029 - RR42_RS22485 0.41 +0.5 382,277 + RR42_RS22485 0.67 +0.3 382,278 - RR42_RS22485 0.67 +0.4 382,322 + RR42_RS22485 0.72 +1.3 382,323 - RR42_RS22485 0.72 +0.3 382,356 - RR42_RS22485 0.75 +0.1 382,373 + RR42_RS22485 0.77 +0.3 382,374 - RR42_RS22485 0.77 +0.3 382,512 - +0.6 382,517 + +0.8 382,517 + -1.3 382,518 - +0.2
Or see this region's nucleotide sequence