Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22455 and RR42_RS22460 are separated by 146 nucleotides RR42_RS22460 and RR42_RS22465 are separated by 67 nucleotides RR42_RS22465 and RR42_RS22470 are separated by 173 nucleotides
RR42_RS22455: RR42_RS22455 - 2-aminoethylphosphonate aminotransferase, at 373,963 to 375,027
_RS22455
RR42_RS22460: RR42_RS22460 - ABC transporter, at 375,174 to 375,872
_RS22460
RR42_RS22465: RR42_RS22465 - membrane protein, at 375,940 to 377,994
_RS22465
RR42_RS22470: RR42_RS22470 - MFS transporter, at 378,168 to 379,493
_RS22470
Position (kb)
375
376
377
378 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 374.970 kb on + strand at 375.026 kb on - strand at 375.050 kb on + strand at 375.101 kb on + strand at 375.101 kb on + strand at 375.102 kb on - strand at 375.102 kb on - strand at 375.102 kb on - strand at 375.102 kb on - strand at 375.102 kb on - strand at 375.107 kb on + strand at 375.107 kb on + strand at 375.108 kb on - strand at 375.108 kb on - strand at 375.260 kb on + strand, within RR42_RS22460 at 375.471 kb on + strand, within RR42_RS22460 at 375.472 kb on - strand, within RR42_RS22460 at 375.664 kb on + strand, within RR42_RS22460 at 375.664 kb on + strand, within RR42_RS22460 at 375.664 kb on + strand, within RR42_RS22460 at 375.665 kb on - strand, within RR42_RS22460 at 375.665 kb on - strand, within RR42_RS22460 at 375.761 kb on + strand, within RR42_RS22460 at 375.761 kb on + strand, within RR42_RS22460 at 375.762 kb on - strand, within RR42_RS22460 at 375.847 kb on - strand at 375.847 kb on - strand at 375.860 kb on + strand at 375.861 kb on - strand at 375.875 kb on + strand at 375.887 kb on + strand at 375.887 kb on + strand at 375.887 kb on + strand at 375.887 kb on + strand at 375.888 kb on - strand at 375.888 kb on - strand at 375.925 kb on + strand at 375.925 kb on + strand at 375.933 kb on + strand at 376.065 kb on + strand at 376.193 kb on + strand, within RR42_RS22465 at 376.194 kb on - strand, within RR42_RS22465 at 376.634 kb on + strand, within RR42_RS22465 at 376.635 kb on - strand, within RR42_RS22465 at 376.766 kb on + strand, within RR42_RS22465 at 376.766 kb on + strand, within RR42_RS22465 at 376.767 kb on - strand, within RR42_RS22465 at 376.823 kb on + strand, within RR42_RS22465 at 376.824 kb on - strand, within RR42_RS22465 at 377.060 kb on + strand, within RR42_RS22465 at 377.061 kb on - strand, within RR42_RS22465 at 377.061 kb on - strand, within RR42_RS22465 at 377.214 kb on - strand, within RR42_RS22465 at 377.427 kb on - strand, within RR42_RS22465 at 377.585 kb on + strand, within RR42_RS22465 at 377.585 kb on + strand, within RR42_RS22465 at 377.585 kb on + strand, within RR42_RS22465 at 377.586 kb on - strand, within RR42_RS22465 at 377.588 kb on + strand, within RR42_RS22465 at 377.588 kb on + strand, within RR42_RS22465 at 377.588 kb on + strand, within RR42_RS22465 at 377.589 kb on - strand, within RR42_RS22465 at 377.589 kb on - strand, within RR42_RS22465 at 377.627 kb on + strand, within RR42_RS22465 at 377.628 kb on - strand, within RR42_RS22465 at 377.927 kb on + strand at 377.928 kb on - strand at 377.978 kb on + strand at 377.979 kb on - strand at 377.983 kb on - strand at 378.244 kb on + strand at 378.244 kb on + strand at 378.245 kb on - strand at 378.245 kb on - strand at 378.245 kb on - strand at 378.335 kb on + strand, within RR42_RS22470 at 378.608 kb on - strand, within RR42_RS22470 at 378.707 kb on + strand, within RR42_RS22470 at 378.707 kb on + strand, within RR42_RS22470 at 378.708 kb on - strand, within RR42_RS22470 at 378.721 kb on + strand, within RR42_RS22470 at 378.722 kb on - strand, within RR42_RS22470 at 378.934 kb on + strand, within RR42_RS22470 at 378.935 kb on - strand, within RR42_RS22470
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 374,970 + -0.1 375,026 - +1.0 375,050 + +0.5 375,101 + -0.2 375,101 + -0.8 375,102 - +2.3 375,102 - -0.5 375,102 - +0.0 375,102 - +0.6 375,102 - -0.6 375,107 + +0.5 375,107 + -1.3 375,108 - -0.8 375,108 - +0.6 375,260 + RR42_RS22460 0.12 -0.5 375,471 + RR42_RS22460 0.42 +0.2 375,472 - RR42_RS22460 0.43 +1.8 375,664 + RR42_RS22460 0.70 +0.1 375,664 + RR42_RS22460 0.70 -1.5 375,664 + RR42_RS22460 0.70 -0.3 375,665 - RR42_RS22460 0.70 -0.8 375,665 - RR42_RS22460 0.70 +0.1 375,761 + RR42_RS22460 0.84 -0.0 375,761 + RR42_RS22460 0.84 -0.1 375,762 - RR42_RS22460 0.84 -0.3 375,847 - +0.4 375,847 - +0.7 375,860 + -0.7 375,861 - +0.1 375,875 + +0.4 375,887 + +0.4 375,887 + -0.5 375,887 + +0.3 375,887 + -1.3 375,888 - -0.8 375,888 - +1.1 375,925 + -0.1 375,925 + -0.4 375,933 + +2.0 376,065 + -1.2 376,193 + RR42_RS22465 0.12 -1.1 376,194 - RR42_RS22465 0.12 +1.0 376,634 + RR42_RS22465 0.34 -1.1 376,635 - RR42_RS22465 0.34 -1.3 376,766 + RR42_RS22465 0.40 -0.3 376,766 + RR42_RS22465 0.40 -0.3 376,767 - RR42_RS22465 0.40 -0.4 376,823 + RR42_RS22465 0.43 -0.1 376,824 - RR42_RS22465 0.43 -0.8 377,060 + RR42_RS22465 0.55 +1.3 377,061 - RR42_RS22465 0.55 -2.0 377,061 - RR42_RS22465 0.55 -0.8 377,214 - RR42_RS22465 0.62 -0.2 377,427 - RR42_RS22465 0.72 +0.9 377,585 + RR42_RS22465 0.80 -0.7 377,585 + RR42_RS22465 0.80 +1.5 377,585 + RR42_RS22465 0.80 +0.6 377,586 - RR42_RS22465 0.80 -0.2 377,588 + RR42_RS22465 0.80 +0.2 377,588 + RR42_RS22465 0.80 -1.2 377,588 + RR42_RS22465 0.80 +0.2 377,589 - RR42_RS22465 0.80 +1.9 377,589 - RR42_RS22465 0.80 +1.6 377,627 + RR42_RS22465 0.82 -0.5 377,628 - RR42_RS22465 0.82 +1.4 377,927 + -0.5 377,928 - +0.8 377,978 + +1.6 377,979 - -0.4 377,983 - -1.9 378,244 + +0.6 378,244 + +0.5 378,245 - -0.4 378,245 - -1.4 378,245 - -1.3 378,335 + RR42_RS22470 0.13 +0.4 378,608 - RR42_RS22470 0.33 -0.4 378,707 + RR42_RS22470 0.41 +0.1 378,707 + RR42_RS22470 0.41 +0.3 378,708 - RR42_RS22470 0.41 -0.5 378,721 + RR42_RS22470 0.42 +1.3 378,722 - RR42_RS22470 0.42 -0.6 378,934 + RR42_RS22470 0.58 +1.2 378,935 - RR42_RS22470 0.58 -1.0
Or see this region's nucleotide sequence