Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22290 and RR42_RS22295 are separated by 50 nucleotides RR42_RS22295 and RR42_RS22300 are separated by 226 nucleotides RR42_RS22300 and RR42_RS22305 are separated by 20 nucleotides
RR42_RS22290: RR42_RS22290 - cytochrome C biogenesis protein, at 341,415 to 343,223
_RS22290
RR42_RS22295: RR42_RS22295 - methionine sulfoxide reductase B, at 343,274 to 343,813
_RS22295
RR42_RS22300: RR42_RS22300 - chemotaxis protein CheY, at 344,040 to 344,762
_RS22300
RR42_RS22305: RR42_RS22305 - histidine kinase, at 344,783 to 346,276
_RS22305
Position (kb) 343
344 Strain fitness (log2 ratio) -2
-1
0
1
2 at 342.286 kb on + strand, within RR42_RS22290 at 342.287 kb on - strand, within RR42_RS22290 at 342.500 kb on + strand, within RR42_RS22290 at 343.202 kb on - strand at 343.211 kb on + strand at 343.211 kb on + strand at 343.436 kb on + strand, within RR42_RS22295 at 343.659 kb on - strand, within RR42_RS22295 at 343.835 kb on + strand at 343.835 kb on + strand at 343.893 kb on + strand at 343.894 kb on - strand at 343.894 kb on - strand at 343.934 kb on + strand at 343.972 kb on - strand at 344.095 kb on + strand at 344.095 kb on + strand at 344.096 kb on - strand at 344.096 kb on - strand at 344.124 kb on + strand, within RR42_RS22300 at 344.125 kb on - strand, within RR42_RS22300 at 344.265 kb on + strand, within RR42_RS22300 at 344.266 kb on - strand, within RR42_RS22300 at 344.343 kb on + strand, within RR42_RS22300 at 344.343 kb on + strand, within RR42_RS22300 at 344.344 kb on - strand, within RR42_RS22300 at 344.544 kb on + strand, within RR42_RS22300 at 344.633 kb on + strand, within RR42_RS22300 at 344.633 kb on + strand, within RR42_RS22300 at 344.634 kb on - strand, within RR42_RS22300 at 344.634 kb on - strand, within RR42_RS22300 at 344.648 kb on + strand, within RR42_RS22300 at 344.648 kb on + strand, within RR42_RS22300 at 344.648 kb on + strand, within RR42_RS22300 at 344.724 kb on + strand at 344.724 kb on + strand at 344.725 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 342,286 + RR42_RS22290 0.48 -0.8 342,287 - RR42_RS22290 0.48 +0.6 342,500 + RR42_RS22290 0.60 +0.6 343,202 - -0.9 343,211 + -0.5 343,211 + -0.1 343,436 + RR42_RS22295 0.30 -0.7 343,659 - RR42_RS22295 0.71 -0.4 343,835 + -0.7 343,835 + +0.6 343,893 + -0.1 343,894 - +1.3 343,894 - -0.8 343,934 + -1.2 343,972 - +0.0 344,095 + -0.6 344,095 + -0.3 344,096 - +0.2 344,096 - +0.2 344,124 + RR42_RS22300 0.12 +0.6 344,125 - RR42_RS22300 0.12 -0.1 344,265 + RR42_RS22300 0.31 +0.6 344,266 - RR42_RS22300 0.31 -0.4 344,343 + RR42_RS22300 0.42 -0.9 344,343 + RR42_RS22300 0.42 +0.2 344,344 - RR42_RS22300 0.42 +1.9 344,544 + RR42_RS22300 0.70 +1.3 344,633 + RR42_RS22300 0.82 +0.6 344,633 + RR42_RS22300 0.82 +0.5 344,634 - RR42_RS22300 0.82 -1.9 344,634 - RR42_RS22300 0.82 +0.6 344,648 + RR42_RS22300 0.84 +0.5 344,648 + RR42_RS22300 0.84 +1.6 344,648 + RR42_RS22300 0.84 -0.1 344,724 + +0.1 344,724 + +1.6 344,725 - +0.3
Or see this region's nucleotide sequence