Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22245 and RR42_RS22250 are separated by 25 nucleotides RR42_RS22250 and RR42_RS22255 are separated by 23 nucleotides
RR42_RS22245: RR42_RS22245 - cytochrome C biogenesis protein, at 329,373 to 330,320
_RS22245
RR42_RS22250: RR42_RS22250 - hypothetical protein, at 330,346 to 330,642
_RS22250
RR42_RS22255: RR42_RS22255 - hypothetical protein, at 330,666 to 332,096
_RS22255
Position (kb) 330
331 Strain fitness (log2 ratio) -1
0
1
2 at 329.379 kb on + strand at 329.380 kb on - strand at 329.391 kb on + strand at 329.392 kb on - strand at 329.466 kb on + strand at 329.467 kb on - strand at 329.467 kb on - strand at 329.529 kb on + strand, within RR42_RS22245 at 329.530 kb on - strand, within RR42_RS22245 at 329.587 kb on - strand, within RR42_RS22245 at 329.640 kb on + strand, within RR42_RS22245 at 329.640 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.983 kb on - strand, within RR42_RS22245 at 330.284 kb on + strand at 330.284 kb on + strand at 330.341 kb on + strand at 330.342 kb on - strand at 330.609 kb on - strand, within RR42_RS22250 at 330.718 kb on + strand at 330.718 kb on + strand at 330.718 kb on + strand at 330.718 kb on + strand at 330.719 kb on - strand at 330.719 kb on - strand at 330.719 kb on - strand at 330.947 kb on - strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.333 kb on - strand, within RR42_RS22255 at 331.333 kb on - strand, within RR42_RS22255 at 331.544 kb on - strand, within RR42_RS22255 at 331.544 kb on - strand, within RR42_RS22255 at 331.564 kb on + strand, within RR42_RS22255 at 331.565 kb on - strand, within RR42_RS22255 at 331.565 kb on - strand, within RR42_RS22255 at 331.631 kb on - strand, within RR42_RS22255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 329,379 + -1.3 329,380 - +0.1 329,391 + -0.5 329,392 - +0.3 329,466 + +0.9 329,467 - +0.7 329,467 - +1.5 329,529 + RR42_RS22245 0.16 +0.9 329,530 - RR42_RS22245 0.17 -0.1 329,587 - RR42_RS22245 0.23 -1.4 329,640 + RR42_RS22245 0.28 +1.6 329,640 + RR42_RS22245 0.28 +1.6 329,834 + RR42_RS22245 0.49 +0.8 329,834 + RR42_RS22245 0.49 +0.5 329,983 - RR42_RS22245 0.64 +2.0 330,284 + +0.7 330,284 + +0.8 330,341 + +0.2 330,342 - +0.1 330,609 - RR42_RS22250 0.89 -0.6 330,718 + +0.4 330,718 + +0.0 330,718 + -1.7 330,718 + -0.1 330,719 - +0.3 330,719 - +0.4 330,719 - -0.8 330,947 - RR42_RS22255 0.20 -1.0 331,332 + RR42_RS22255 0.47 -1.7 331,332 + RR42_RS22255 0.47 -0.5 331,332 + RR42_RS22255 0.47 -0.7 331,333 - RR42_RS22255 0.47 -1.2 331,333 - RR42_RS22255 0.47 -0.6 331,544 - RR42_RS22255 0.61 -0.3 331,544 - RR42_RS22255 0.61 +0.6 331,564 + RR42_RS22255 0.63 +0.0 331,565 - RR42_RS22255 0.63 +0.7 331,565 - RR42_RS22255 0.63 +0.3 331,631 - RR42_RS22255 0.67 -1.1
Or see this region's nucleotide sequence