Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21750 and RR42_RS21755 are separated by 370 nucleotides RR42_RS21755 and RR42_RS21760 are separated by 105 nucleotides
RR42_RS21750: RR42_RS21750 - carnitine dehydratase, at 207,952 to 209,109
_RS21750
RR42_RS21755: RR42_RS21755 - shikimate dehydrogenase, at 209,480 to 210,316
_RS21755
RR42_RS21760: RR42_RS21760 - succinate-semialdehyde dehydrogenase, at 210,422 to 211,870
_RS21760
Position (kb) 209
210
211 Strain fitness (log2 ratio) -2
-1
0
1
2 at 208.651 kb on + strand, within RR42_RS21750 at 208.660 kb on + strand, within RR42_RS21750 at 208.661 kb on - strand, within RR42_RS21750 at 208.661 kb on - strand, within RR42_RS21750 at 208.661 kb on - strand, within RR42_RS21750 at 208.661 kb on - strand, within RR42_RS21750 at 208.682 kb on - strand, within RR42_RS21750 at 208.682 kb on - strand, within RR42_RS21750 at 209.111 kb on - strand at 209.111 kb on - strand at 209.111 kb on - strand at 209.225 kb on + strand at 209.226 kb on - strand at 209.226 kb on - strand at 209.226 kb on - strand at 209.226 kb on - strand at 209.291 kb on + strand at 209.396 kb on - strand at 209.461 kb on + strand at 209.675 kb on + strand, within RR42_RS21755 at 209.675 kb on + strand, within RR42_RS21755 at 209.848 kb on - strand, within RR42_RS21755 at 210.445 kb on - strand at 210.445 kb on - strand at 210.459 kb on + strand at 210.459 kb on + strand at 210.460 kb on - strand at 210.460 kb on - strand at 210.571 kb on - strand, within RR42_RS21760 at 210.571 kb on - strand, within RR42_RS21760 at 210.764 kb on - strand, within RR42_RS21760 at 210.844 kb on + strand, within RR42_RS21760 at 210.987 kb on + strand, within RR42_RS21760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 208,651 + RR42_RS21750 0.60 +0.8 208,660 + RR42_RS21750 0.61 -0.8 208,661 - RR42_RS21750 0.61 -0.5 208,661 - RR42_RS21750 0.61 +0.0 208,661 - RR42_RS21750 0.61 -0.2 208,661 - RR42_RS21750 0.61 -0.3 208,682 - RR42_RS21750 0.63 -0.8 208,682 - RR42_RS21750 0.63 +1.1 209,111 - -0.3 209,111 - +1.3 209,111 - -0.4 209,225 + -1.7 209,226 - +0.5 209,226 - -0.1 209,226 - -2.2 209,226 - +0.2 209,291 + +1.0 209,396 - +0.4 209,461 + +0.1 209,675 + RR42_RS21755 0.23 -1.1 209,675 + RR42_RS21755 0.23 +2.6 209,848 - RR42_RS21755 0.44 +0.4 210,445 - +0.6 210,445 - +0.3 210,459 + +1.7 210,459 + -0.6 210,460 - +0.1 210,460 - -0.5 210,571 - RR42_RS21760 0.10 -0.6 210,571 - RR42_RS21760 0.10 -0.5 210,764 - RR42_RS21760 0.24 +0.4 210,844 + RR42_RS21760 0.29 +0.5 210,987 + RR42_RS21760 0.39 -0.6
Or see this region's nucleotide sequence