Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21625 and RR42_RS21630 are separated by 381 nucleotides RR42_RS21630 and RR42_RS21635 are separated by 28 nucleotides RR42_RS21635 and RR42_RS21640 are separated by 29 nucleotides
RR42_RS21625: RR42_RS21625 - 6-hydroxynicotinate 3-monooxygenase, at 181,169 to 182,323
_RS21625
RR42_RS21630: RR42_RS21630 - Asp/Glu racemase, at 182,705 to 183,457
_RS21630
RR42_RS21635: RR42_RS21635 - alpha/beta hydrolase, at 183,486 to 184,328
_RS21635
RR42_RS21640: RR42_RS21640 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 184,358 to 185,458
_RS21640
Position (kb) 182
183
184 Strain fitness (log2 ratio) -1
0
1
2 at 182.044 kb on - strand, within RR42_RS21625 at 182.226 kb on + strand at 182.227 kb on - strand at 182.500 kb on + strand at 182.500 kb on + strand at 182.500 kb on + strand at 182.501 kb on - strand at 182.501 kb on - strand at 182.501 kb on - strand at 182.975 kb on + strand, within RR42_RS21630 at 183.114 kb on - strand, within RR42_RS21630 at 183.116 kb on - strand, within RR42_RS21630 at 183.383 kb on + strand at 183.383 kb on + strand at 183.452 kb on + strand at 183.665 kb on + strand, within RR42_RS21635 at 183.666 kb on - strand, within RR42_RS21635 at 183.666 kb on - strand, within RR42_RS21635 at 183.669 kb on + strand, within RR42_RS21635 at 183.669 kb on + strand, within RR42_RS21635 at 183.669 kb on + strand, within RR42_RS21635 at 183.671 kb on + strand, within RR42_RS21635 at 183.910 kb on - strand, within RR42_RS21635 at 183.931 kb on - strand, within RR42_RS21635 at 184.365 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 182,044 - RR42_RS21625 0.76 -1.4 182,226 + +1.1 182,227 - -0.7 182,500 + -0.1 182,500 + -1.0 182,500 + -0.7 182,501 - +0.8 182,501 - -0.8 182,501 - +1.2 182,975 + RR42_RS21630 0.36 +0.2 183,114 - RR42_RS21630 0.54 -0.9 183,116 - RR42_RS21630 0.55 -1.7 183,383 + +2.3 183,383 + -0.1 183,452 + +1.3 183,665 + RR42_RS21635 0.21 +1.0 183,666 - RR42_RS21635 0.21 +1.3 183,666 - RR42_RS21635 0.21 -0.7 183,669 + RR42_RS21635 0.22 -1.7 183,669 + RR42_RS21635 0.22 +0.3 183,669 + RR42_RS21635 0.22 +0.2 183,671 + RR42_RS21635 0.22 -1.2 183,910 - RR42_RS21635 0.50 -0.1 183,931 - RR42_RS21635 0.53 +0.7 184,365 + +0.7
Or see this region's nucleotide sequence