Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21585 and RR42_RS21590 are separated by 457 nucleotides RR42_RS21590 and RR42_RS21595 overlap by 4 nucleotides RR42_RS21595 and RR42_RS21600 are separated by 144 nucleotides
RR42_RS21585: RR42_RS21585 - membrane protein, at 168,250 to 169,857
_RS21585
RR42_RS21590: RR42_RS21590 - hypothetical protein, at 170,315 to 173,065
_RS21590
RR42_RS21595: RR42_RS21595 - IclR family transcriptional regulator, at 173,062 to 173,826
_RS21595
RR42_RS21600: RR42_RS21600 - inorganic pyrophosphatase/exopolyphosphatase, at 173,971 to 174,999
_RS21600
Position (kb) 170
171
172
173
174 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.454 kb on + strand, within RR42_RS21585 at 169.455 kb on - strand, within RR42_RS21585 at 169.535 kb on + strand, within RR42_RS21585 at 169.863 kb on + strand at 169.864 kb on - strand at 169.984 kb on + strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 169.985 kb on - strand at 170.141 kb on + strand at 170.142 kb on - strand at 170.236 kb on + strand at 170.237 kb on - strand at 170.237 kb on - strand at 170.238 kb on + strand at 170.238 kb on + strand at 170.238 kb on + strand at 170.238 kb on + strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.239 kb on - strand at 170.464 kb on + strand at 170.464 kb on + strand at 170.579 kb on + strand at 170.606 kb on + strand, within RR42_RS21590 at 170.690 kb on + strand, within RR42_RS21590 at 170.742 kb on - strand, within RR42_RS21590 at 170.829 kb on - strand, within RR42_RS21590 at 170.870 kb on + strand, within RR42_RS21590 at 170.870 kb on + strand, within RR42_RS21590 at 170.870 kb on + strand, within RR42_RS21590 at 170.870 kb on + strand, within RR42_RS21590 at 170.871 kb on - strand, within RR42_RS21590 at 170.871 kb on - strand, within RR42_RS21590 at 170.894 kb on + strand, within RR42_RS21590 at 171.090 kb on - strand, within RR42_RS21590 at 171.422 kb on + strand, within RR42_RS21590 at 171.423 kb on - strand, within RR42_RS21590 at 171.423 kb on - strand, within RR42_RS21590 at 171.423 kb on - strand, within RR42_RS21590 at 171.423 kb on - strand, within RR42_RS21590 at 171.506 kb on + strand, within RR42_RS21590 at 171.506 kb on + strand, within RR42_RS21590 at 171.689 kb on + strand, within RR42_RS21590 at 171.794 kb on + strand, within RR42_RS21590 at 171.794 kb on + strand, within RR42_RS21590 at 171.950 kb on + strand, within RR42_RS21590 at 171.951 kb on - strand, within RR42_RS21590 at 171.951 kb on - strand, within RR42_RS21590 at 171.951 kb on - strand, within RR42_RS21590 at 171.953 kb on + strand, within RR42_RS21590 at 171.953 kb on + strand, within RR42_RS21590 at 171.953 kb on + strand, within RR42_RS21590 at 171.953 kb on + strand, within RR42_RS21590 at 171.953 kb on + strand, within RR42_RS21590 at 171.954 kb on - strand, within RR42_RS21590 at 171.954 kb on - strand, within RR42_RS21590 at 171.954 kb on - strand, within RR42_RS21590 at 172.128 kb on + strand, within RR42_RS21590 at 172.129 kb on - strand, within RR42_RS21590 at 172.155 kb on - strand, within RR42_RS21590 at 172.448 kb on + strand, within RR42_RS21590 at 172.451 kb on + strand, within RR42_RS21590 at 172.451 kb on + strand, within RR42_RS21590 at 172.505 kb on + strand, within RR42_RS21590 at 172.505 kb on + strand, within RR42_RS21590 at 172.505 kb on + strand, within RR42_RS21590 at 172.544 kb on + strand, within RR42_RS21590 at 172.545 kb on - strand, within RR42_RS21590 at 172.613 kb on + strand, within RR42_RS21590 at 172.613 kb on + strand, within RR42_RS21590 at 172.901 kb on + strand at 172.976 kb on + strand at 172.977 kb on - strand at 172.977 kb on - strand at 173.646 kb on + strand, within RR42_RS21595 at 173.646 kb on - strand, within RR42_RS21595 at 173.784 kb on - strand at 173.826 kb on + strand at 173.826 kb on + strand at 173.826 kb on + strand at 173.869 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 169,454 + RR42_RS21585 0.75 -1.4 169,454 + RR42_RS21585 0.75 -0.5 169,454 + RR42_RS21585 0.75 +1.3 169,454 + RR42_RS21585 0.75 -0.4 169,454 + RR42_RS21585 0.75 +0.1 169,454 + RR42_RS21585 0.75 -1.0 169,455 - RR42_RS21585 0.75 +0.7 169,535 + RR42_RS21585 0.80 +0.2 169,863 + -0.6 169,864 - -0.3 169,984 + +1.0 169,985 - -2.7 169,985 - -1.2 169,985 - -0.7 169,985 - -0.6 169,985 - -0.3 169,985 - -1.2 169,985 - +0.3 169,985 - +0.2 169,985 - -1.1 169,985 - -0.9 169,985 - -0.1 170,141 + -0.6 170,142 - -0.5 170,236 + -0.7 170,237 - +0.4 170,237 - -0.8 170,238 + -1.0 170,238 + +1.0 170,238 + +0.2 170,238 + +0.7 170,239 - -0.1 170,239 - -0.0 170,239 - -1.5 170,239 - +0.5 170,239 - -1.9 170,239 - +0.7 170,239 - +0.7 170,239 - -0.6 170,239 - +0.9 170,239 - +0.3 170,239 - +0.6 170,239 - +0.5 170,464 + +1.3 170,464 + -0.1 170,579 + -0.4 170,606 + RR42_RS21590 0.11 -0.3 170,690 + RR42_RS21590 0.14 +0.2 170,742 - RR42_RS21590 0.16 +0.8 170,829 - RR42_RS21590 0.19 -0.4 170,870 + RR42_RS21590 0.20 -0.1 170,870 + RR42_RS21590 0.20 +0.1 170,870 + RR42_RS21590 0.20 -0.1 170,870 + RR42_RS21590 0.20 -1.6 170,871 - RR42_RS21590 0.20 +1.3 170,871 - RR42_RS21590 0.20 -0.7 170,894 + RR42_RS21590 0.21 +0.1 171,090 - RR42_RS21590 0.28 +0.6 171,422 + RR42_RS21590 0.40 -0.1 171,423 - RR42_RS21590 0.40 -2.2 171,423 - RR42_RS21590 0.40 +0.1 171,423 - RR42_RS21590 0.40 -1.6 171,423 - RR42_RS21590 0.40 -0.6 171,506 + RR42_RS21590 0.43 +0.3 171,506 + RR42_RS21590 0.43 -0.0 171,689 + RR42_RS21590 0.50 +1.3 171,794 + RR42_RS21590 0.54 +0.7 171,794 + RR42_RS21590 0.54 -0.5 171,950 + RR42_RS21590 0.59 -0.1 171,951 - RR42_RS21590 0.59 -0.1 171,951 - RR42_RS21590 0.59 +0.3 171,951 - RR42_RS21590 0.59 -0.0 171,953 + RR42_RS21590 0.60 -0.7 171,953 + RR42_RS21590 0.60 +1.0 171,953 + RR42_RS21590 0.60 -0.2 171,953 + RR42_RS21590 0.60 +0.7 171,953 + RR42_RS21590 0.60 +0.2 171,954 - RR42_RS21590 0.60 -0.0 171,954 - RR42_RS21590 0.60 -0.8 171,954 - RR42_RS21590 0.60 -1.1 172,128 + RR42_RS21590 0.66 -0.8 172,129 - RR42_RS21590 0.66 -0.4 172,155 - RR42_RS21590 0.67 -0.6 172,448 + RR42_RS21590 0.78 -0.5 172,451 + RR42_RS21590 0.78 +0.2 172,451 + RR42_RS21590 0.78 -1.5 172,505 + RR42_RS21590 0.80 +0.7 172,505 + RR42_RS21590 0.80 -0.4 172,505 + RR42_RS21590 0.80 -0.7 172,544 + RR42_RS21590 0.81 -0.8 172,545 - RR42_RS21590 0.81 -0.1 172,613 + RR42_RS21590 0.84 -0.4 172,613 + RR42_RS21590 0.84 -0.9 172,901 + +1.0 172,976 + -0.3 172,977 - +0.3 172,977 - +0.5 173,646 + RR42_RS21595 0.76 -0.4 173,646 - RR42_RS21595 0.76 -1.8 173,784 - -0.0 173,826 + +0.4 173,826 + -2.9 173,826 + -0.7 173,869 + -0.2
Or see this region's nucleotide sequence