Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21420 and RR42_RS21425 are separated by 149 nucleotides RR42_RS21425 and RR42_RS21430 are separated by 12 nucleotides
RR42_RS21420: RR42_RS21420 - acyl-CoA dehydrogenase, at 134,799 to 135,956
_RS21420
RR42_RS21425: RR42_RS21425 - hypothetical protein, at 136,106 to 136,963
_RS21425
RR42_RS21430: RR42_RS21430 - alpha-ketoglutarate permease, at 136,976 to 138,286
_RS21430
Position (kb)
136
137 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 135.123 kb on + strand, within RR42_RS21420 at 135.123 kb on + strand, within RR42_RS21420 at 135.237 kb on + strand, within RR42_RS21420 at 135.237 kb on + strand, within RR42_RS21420 at 135.238 kb on - strand, within RR42_RS21420 at 135.238 kb on - strand, within RR42_RS21420 at 135.293 kb on + strand, within RR42_RS21420 at 135.530 kb on + strand, within RR42_RS21420 at 135.531 kb on - strand, within RR42_RS21420 at 135.570 kb on - strand, within RR42_RS21420 at 135.585 kb on + strand, within RR42_RS21420 at 135.586 kb on - strand, within RR42_RS21420 at 135.586 kb on - strand, within RR42_RS21420 at 135.656 kb on + strand, within RR42_RS21420 at 135.672 kb on + strand, within RR42_RS21420 at 135.840 kb on + strand, within RR42_RS21420 at 135.840 kb on + strand, within RR42_RS21420 at 135.841 kb on - strand at 135.842 kb on + strand at 135.842 kb on + strand at 135.843 kb on - strand at 135.853 kb on - strand at 135.881 kb on - strand at 135.881 kb on - strand at 135.898 kb on - strand at 136.020 kb on + strand at 136.139 kb on + strand at 136.289 kb on + strand, within RR42_RS21425 at 136.289 kb on + strand, within RR42_RS21425 at 136.290 kb on - strand, within RR42_RS21425 at 136.404 kb on - strand, within RR42_RS21425 at 136.436 kb on + strand, within RR42_RS21425 at 136.481 kb on + strand, within RR42_RS21425 at 136.481 kb on + strand, within RR42_RS21425 at 136.481 kb on + strand, within RR42_RS21425 at 136.481 kb on + strand, within RR42_RS21425 at 136.523 kb on + strand, within RR42_RS21425 at 136.523 kb on + strand, within RR42_RS21425 at 136.589 kb on + strand, within RR42_RS21425 at 136.590 kb on - strand, within RR42_RS21425 at 136.590 kb on - strand, within RR42_RS21425 at 136.590 kb on - strand, within RR42_RS21425 at 136.730 kb on + strand, within RR42_RS21425 at 136.730 kb on + strand, within RR42_RS21425 at 136.731 kb on - strand, within RR42_RS21425 at 136.731 kb on - strand, within RR42_RS21425 at 136.732 kb on + strand, within RR42_RS21425 at 136.732 kb on + strand, within RR42_RS21425 at 136.733 kb on - strand, within RR42_RS21425 at 136.995 kb on + strand at 136.996 kb on - strand at 137.059 kb on - strand at 137.064 kb on + strand at 137.065 kb on - strand at 137.065 kb on - strand at 137.065 kb on - strand at 137.065 kb on - strand at 137.110 kb on - strand, within RR42_RS21430 at 137.148 kb on + strand, within RR42_RS21430 at 137.148 kb on + strand, within RR42_RS21430 at 137.148 kb on + strand, within RR42_RS21430 at 137.149 kb on - strand, within RR42_RS21430 at 137.223 kb on + strand, within RR42_RS21430 at 137.224 kb on - strand, within RR42_RS21430 at 137.224 kb on - strand, within RR42_RS21430 at 137.347 kb on + strand, within RR42_RS21430 at 137.347 kb on + strand, within RR42_RS21430 at 137.348 kb on - strand, within RR42_RS21430 at 137.348 kb on - strand, within RR42_RS21430 at 137.348 kb on - strand, within RR42_RS21430 at 137.406 kb on + strand, within RR42_RS21430 at 137.406 kb on + strand, within RR42_RS21430 at 137.406 kb on + strand, within RR42_RS21430 at 137.406 kb on + strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.433 kb on + strand, within RR42_RS21430 at 137.433 kb on + strand, within RR42_RS21430 at 137.433 kb on + strand, within RR42_RS21430 at 137.434 kb on - strand, within RR42_RS21430 at 137.434 kb on - strand, within RR42_RS21430 at 137.485 kb on + strand, within RR42_RS21430 at 137.485 kb on + strand, within RR42_RS21430 at 137.486 kb on - strand, within RR42_RS21430 at 137.486 kb on - strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.500 kb on + strand, within RR42_RS21430 at 137.502 kb on + strand, within RR42_RS21430 at 137.503 kb on - strand, within RR42_RS21430 at 137.503 kb on - strand, within RR42_RS21430 at 137.527 kb on + strand, within RR42_RS21430 at 137.527 kb on + strand, within RR42_RS21430 at 137.530 kb on - strand, within RR42_RS21430 at 137.557 kb on + strand, within RR42_RS21430 at 137.557 kb on + strand, within RR42_RS21430 at 137.557 kb on + strand, within RR42_RS21430 at 137.558 kb on - strand, within RR42_RS21430 at 137.558 kb on - strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.860 kb on - strand, within RR42_RS21430 at 137.937 kb on + strand, within RR42_RS21430 at 137.937 kb on + strand, within RR42_RS21430 at 137.938 kb on - strand, within RR42_RS21430 at 137.938 kb on - strand, within RR42_RS21430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 135,123 + RR42_RS21420 0.28 +3.5 135,123 + RR42_RS21420 0.28 -0.0 135,237 + RR42_RS21420 0.38 -1.3 135,237 + RR42_RS21420 0.38 +0.2 135,238 - RR42_RS21420 0.38 -0.4 135,238 - RR42_RS21420 0.38 -1.5 135,293 + RR42_RS21420 0.43 +0.6 135,530 + RR42_RS21420 0.63 +1.1 135,531 - RR42_RS21420 0.63 -0.8 135,570 - RR42_RS21420 0.67 +0.3 135,585 + RR42_RS21420 0.68 +0.7 135,586 - RR42_RS21420 0.68 +1.2 135,586 - RR42_RS21420 0.68 +0.0 135,656 + RR42_RS21420 0.74 -1.2 135,672 + RR42_RS21420 0.75 +0.4 135,840 + RR42_RS21420 0.90 +0.0 135,840 + RR42_RS21420 0.90 +0.3 135,841 - +0.1 135,842 + -0.0 135,842 + -0.1 135,843 - +0.4 135,853 - -1.1 135,881 - -0.2 135,881 - +0.6 135,898 - +0.4 136,020 + -0.0 136,139 + +0.5 136,289 + RR42_RS21425 0.21 +0.8 136,289 + RR42_RS21425 0.21 +0.5 136,290 - RR42_RS21425 0.21 -0.5 136,404 - RR42_RS21425 0.35 -0.1 136,436 + RR42_RS21425 0.38 +0.3 136,481 + RR42_RS21425 0.44 +0.9 136,481 + RR42_RS21425 0.44 +0.5 136,481 + RR42_RS21425 0.44 +0.1 136,481 + RR42_RS21425 0.44 +0.6 136,523 + RR42_RS21425 0.49 +0.5 136,523 + RR42_RS21425 0.49 +0.8 136,589 + RR42_RS21425 0.56 +1.8 136,590 - RR42_RS21425 0.56 +1.5 136,590 - RR42_RS21425 0.56 +0.6 136,590 - RR42_RS21425 0.56 -1.2 136,730 + RR42_RS21425 0.73 -0.6 136,730 + RR42_RS21425 0.73 -0.0 136,731 - RR42_RS21425 0.73 -0.4 136,731 - RR42_RS21425 0.73 +3.2 136,732 + RR42_RS21425 0.73 -2.0 136,732 + RR42_RS21425 0.73 +0.3 136,733 - RR42_RS21425 0.73 -0.4 136,995 + +0.7 136,996 - +0.2 137,059 - -1.0 137,064 + -0.3 137,065 - -2.3 137,065 - -0.8 137,065 - -0.1 137,065 - -2.3 137,110 - RR42_RS21430 0.10 +0.1 137,148 + RR42_RS21430 0.13 -0.9 137,148 + RR42_RS21430 0.13 -2.9 137,148 + RR42_RS21430 0.13 +1.0 137,149 - RR42_RS21430 0.13 +0.4 137,223 + RR42_RS21430 0.19 +0.9 137,224 - RR42_RS21430 0.19 +0.3 137,224 - RR42_RS21430 0.19 -0.1 137,347 + RR42_RS21430 0.28 +0.0 137,347 + RR42_RS21430 0.28 +0.8 137,348 - RR42_RS21430 0.28 -0.1 137,348 - RR42_RS21430 0.28 +0.1 137,348 - RR42_RS21430 0.28 +0.8 137,406 + RR42_RS21430 0.33 +0.3 137,406 + RR42_RS21430 0.33 +0.2 137,406 + RR42_RS21430 0.33 -1.2 137,406 + RR42_RS21430 0.33 +0.2 137,407 - RR42_RS21430 0.33 -1.7 137,407 - RR42_RS21430 0.33 +0.6 137,407 - RR42_RS21430 0.33 +1.5 137,407 - RR42_RS21430 0.33 -1.4 137,407 - RR42_RS21430 0.33 -0.7 137,407 - RR42_RS21430 0.33 -0.9 137,407 - RR42_RS21430 0.33 -0.2 137,433 + RR42_RS21430 0.35 -0.3 137,433 + RR42_RS21430 0.35 +0.3 137,433 + RR42_RS21430 0.35 -0.6 137,434 - RR42_RS21430 0.35 -1.8 137,434 - RR42_RS21430 0.35 +0.4 137,485 + RR42_RS21430 0.39 +0.2 137,485 + RR42_RS21430 0.39 +0.2 137,486 - RR42_RS21430 0.39 +0.3 137,486 - RR42_RS21430 0.39 -1.2 137,487 + RR42_RS21430 0.39 +0.7 137,487 + RR42_RS21430 0.39 +0.5 137,487 + RR42_RS21430 0.39 -0.1 137,487 + RR42_RS21430 0.39 +0.4 137,487 + RR42_RS21430 0.39 +0.4 137,487 + RR42_RS21430 0.39 -1.2 137,487 + RR42_RS21430 0.39 +0.1 137,487 + RR42_RS21430 0.39 -1.2 137,488 - RR42_RS21430 0.39 -0.9 137,488 - RR42_RS21430 0.39 +0.3 137,488 - RR42_RS21430 0.39 +0.6 137,488 - RR42_RS21430 0.39 +1.7 137,488 - RR42_RS21430 0.39 -0.5 137,500 + RR42_RS21430 0.40 -0.9 137,502 + RR42_RS21430 0.40 -1.4 137,503 - RR42_RS21430 0.40 -0.0 137,503 - RR42_RS21430 0.40 -0.0 137,527 + RR42_RS21430 0.42 +0.6 137,527 + RR42_RS21430 0.42 -1.4 137,530 - RR42_RS21430 0.42 +0.5 137,557 + RR42_RS21430 0.44 +0.5 137,557 + RR42_RS21430 0.44 -1.9 137,557 + RR42_RS21430 0.44 +1.0 137,558 - RR42_RS21430 0.44 -0.6 137,558 - RR42_RS21430 0.44 +0.2 137,839 + RR42_RS21430 0.66 +0.2 137,839 + RR42_RS21430 0.66 -0.5 137,839 + RR42_RS21430 0.66 -0.2 137,839 + RR42_RS21430 0.66 +1.3 137,839 + RR42_RS21430 0.66 -1.4 137,839 + RR42_RS21430 0.66 -0.3 137,840 - RR42_RS21430 0.66 -1.4 137,840 - RR42_RS21430 0.66 -1.3 137,840 - RR42_RS21430 0.66 +0.6 137,840 - RR42_RS21430 0.66 -0.4 137,841 + RR42_RS21430 0.66 -0.7 137,841 + RR42_RS21430 0.66 -1.9 137,841 + RR42_RS21430 0.66 -1.0 137,841 + RR42_RS21430 0.66 -0.3 137,841 + RR42_RS21430 0.66 +0.3 137,841 + RR42_RS21430 0.66 +1.3 137,842 - RR42_RS21430 0.66 -0.4 137,842 - RR42_RS21430 0.66 +0.4 137,842 - RR42_RS21430 0.66 -0.2 137,842 - RR42_RS21430 0.66 +0.1 137,842 - RR42_RS21430 0.66 -0.4 137,860 - RR42_RS21430 0.67 +1.8 137,937 + RR42_RS21430 0.73 -0.5 137,937 + RR42_RS21430 0.73 +0.0 137,938 - RR42_RS21430 0.73 -2.2 137,938 - RR42_RS21430 0.73 +0.4
Or see this region's nucleotide sequence