Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21255 and RR42_RS21260 are separated by 118 nucleotides RR42_RS21260 and RR42_RS21265 are separated by 106 nucleotides
RR42_RS21255: RR42_RS21255 - LysR family transcriptional regulator, at 94,069 to 94,947
_RS21255
RR42_RS21260: RR42_RS21260 - isovaleryl-CoA dehydrogenase, at 95,066 to 96,862
_RS21260
RR42_RS21265: RR42_RS21265 - ABC transporter substrate-binding protein, at 96,969 to 97,949
_RS21265
Position (kb) 95
96
97 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 94.142 kb on + strand at 94.142 kb on + strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.301 kb on + strand, within RR42_RS21255 at 94.340 kb on + strand, within RR42_RS21255 at 94.340 kb on + strand, within RR42_RS21255 at 94.341 kb on - strand, within RR42_RS21255 at 94.344 kb on - strand, within RR42_RS21255 at 94.471 kb on + strand, within RR42_RS21255 at 94.640 kb on + strand, within RR42_RS21255 at 94.641 kb on - strand, within RR42_RS21255 at 94.842 kb on + strand, within RR42_RS21255 at 94.842 kb on + strand, within RR42_RS21255 at 94.925 kb on + strand at 94.962 kb on + strand at 95.010 kb on + strand at 95.013 kb on + strand at 95.013 kb on + strand at 95.013 kb on + strand at 95.014 kb on - strand at 95.027 kb on + strand at 95.060 kb on + strand at 95.061 kb on - strand at 95.061 kb on - strand at 95.198 kb on + strand at 95.198 kb on + strand at 95.199 kb on - strand at 95.287 kb on + strand, within RR42_RS21260 at 95.287 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.474 kb on + strand, within RR42_RS21260 at 95.475 kb on - strand, within RR42_RS21260 at 95.475 kb on - strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.630 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260 at 95.969 kb on + strand, within RR42_RS21260 at 95.969 kb on + strand, within RR42_RS21260 at 96.251 kb on + strand, within RR42_RS21260 at 96.353 kb on + strand, within RR42_RS21260 at 96.353 kb on + strand, within RR42_RS21260 at 96.354 kb on - strand, within RR42_RS21260 at 96.355 kb on + strand, within RR42_RS21260 at 96.355 kb on + strand, within RR42_RS21260 at 96.356 kb on - strand, within RR42_RS21260 at 96.644 kb on + strand, within RR42_RS21260 at 96.645 kb on - strand, within RR42_RS21260 at 96.645 kb on - strand, within RR42_RS21260 at 96.868 kb on + strand at 96.868 kb on + strand at 96.952 kb on + strand at 96.953 kb on - strand at 96.953 kb on - strand at 97.194 kb on - strand, within RR42_RS21265 at 97.233 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.300 kb on - strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.525 kb on - strand, within RR42_RS21265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 94,142 + -0.1 94,142 + +0.3 94,143 - -0.7 94,143 - -1.4 94,143 - -0.3 94,143 - +0.3 94,301 + RR42_RS21255 0.26 +0.7 94,340 + RR42_RS21255 0.31 +2.6 94,340 + RR42_RS21255 0.31 +0.5 94,341 - RR42_RS21255 0.31 -0.1 94,344 - RR42_RS21255 0.31 -0.3 94,471 + RR42_RS21255 0.46 -0.7 94,640 + RR42_RS21255 0.65 -0.7 94,641 - RR42_RS21255 0.65 +0.2 94,842 + RR42_RS21255 0.88 +0.4 94,842 + RR42_RS21255 0.88 +0.1 94,925 + +0.3 94,962 + -0.1 95,010 + -0.9 95,013 + -0.2 95,013 + +0.1 95,013 + -0.2 95,014 - +0.6 95,027 + -0.9 95,060 + +1.3 95,061 - -1.1 95,061 - -0.0 95,198 + -2.0 95,198 + -1.3 95,199 - +0.0 95,287 + RR42_RS21260 0.12 -0.4 95,287 + RR42_RS21260 0.12 -0.9 95,369 + RR42_RS21260 0.17 -0.3 95,369 + RR42_RS21260 0.17 -0.5 95,369 + RR42_RS21260 0.17 +0.1 95,474 + RR42_RS21260 0.23 +2.9 95,475 - RR42_RS21260 0.23 -1.0 95,475 - RR42_RS21260 0.23 -0.0 95,486 + RR42_RS21260 0.23 -1.0 95,486 + RR42_RS21260 0.23 +1.0 95,486 + RR42_RS21260 0.23 -0.7 95,486 + RR42_RS21260 0.23 +1.2 95,486 + RR42_RS21260 0.23 +0.1 95,630 + RR42_RS21260 0.31 -1.1 95,888 + RR42_RS21260 0.46 +0.2 95,888 + RR42_RS21260 0.46 -0.3 95,888 + RR42_RS21260 0.46 -1.4 95,969 + RR42_RS21260 0.50 +0.1 95,969 + RR42_RS21260 0.50 +0.1 96,251 + RR42_RS21260 0.66 -0.0 96,353 + RR42_RS21260 0.72 -0.8 96,353 + RR42_RS21260 0.72 -0.3 96,354 - RR42_RS21260 0.72 -0.7 96,355 + RR42_RS21260 0.72 -0.2 96,355 + RR42_RS21260 0.72 +0.3 96,356 - RR42_RS21260 0.72 +0.3 96,644 + RR42_RS21260 0.88 -0.4 96,645 - RR42_RS21260 0.88 +0.7 96,645 - RR42_RS21260 0.88 -1.0 96,868 + -0.5 96,868 + -0.9 96,952 + -0.0 96,953 - +0.2 96,953 - -0.3 97,194 - RR42_RS21265 0.23 -0.3 97,233 + RR42_RS21265 0.27 +0.4 97,299 + RR42_RS21265 0.34 +0.1 97,299 + RR42_RS21265 0.34 -0.3 97,299 + RR42_RS21265 0.34 +1.0 97,300 - RR42_RS21265 0.34 -2.5 97,437 + RR42_RS21265 0.48 +0.0 97,437 + RR42_RS21265 0.48 +1.9 97,437 + RR42_RS21265 0.48 +0.3 97,437 + RR42_RS21265 0.48 +0.2 97,437 + RR42_RS21265 0.48 +0.8 97,437 + RR42_RS21265 0.48 +2.3 97,437 + RR42_RS21265 0.48 -0.6 97,437 + RR42_RS21265 0.48 +0.1 97,437 + RR42_RS21265 0.48 -2.3 97,438 - RR42_RS21265 0.48 -0.6 97,438 - RR42_RS21265 0.48 +0.1 97,438 - RR42_RS21265 0.48 +1.3 97,438 - RR42_RS21265 0.48 +0.8 97,525 - RR42_RS21265 0.57 -0.2
Or see this region's nucleotide sequence