Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20960 and RR42_RS20965 are separated by 44 nucleotides RR42_RS20965 and RR42_RS20970 are separated by 63 nucleotides
RR42_RS20960: RR42_RS20960 - SAM-dependent methyltransferase, at 30,079 to 30,891
_RS20960
RR42_RS20965: RR42_RS20965 - transferase, at 30,936 to 32,123
_RS20965
RR42_RS20970: RR42_RS20970 - hypothetical protein, at 32,187 to 33,467
_RS20970
Position (kb) 30
31
32
33 Strain fitness (log2 ratio) -2
-1
0
1
2 at 29.957 kb on + strand at 30.126 kb on + strand at 30.307 kb on + strand, within RR42_RS20960 at 30.307 kb on + strand, within RR42_RS20960 at 30.307 kb on + strand, within RR42_RS20960 at 30.307 kb on + strand, within RR42_RS20960 at 30.308 kb on - strand, within RR42_RS20960 at 30.308 kb on - strand, within RR42_RS20960 at 30.308 kb on - strand, within RR42_RS20960 at 30.354 kb on - strand, within RR42_RS20960 at 30.451 kb on + strand, within RR42_RS20960 at 30.452 kb on - strand, within RR42_RS20960 at 30.701 kb on - strand, within RR42_RS20960 at 30.751 kb on - strand, within RR42_RS20960 at 30.865 kb on + strand at 31.180 kb on - strand, within RR42_RS20965 at 31.438 kb on - strand, within RR42_RS20965 at 31.881 kb on - strand, within RR42_RS20965 at 31.881 kb on - strand, within RR42_RS20965 at 32.122 kb on - strand at 32.212 kb on - strand at 32.292 kb on + strand at 32.293 kb on - strand at 32.556 kb on + strand, within RR42_RS20970 at 32.676 kb on + strand, within RR42_RS20970 at 32.776 kb on + strand, within RR42_RS20970 at 32.826 kb on + strand, within RR42_RS20970 at 32.826 kb on + strand, within RR42_RS20970 at 32.827 kb on - strand, within RR42_RS20970 at 32.827 kb on - strand, within RR42_RS20970 at 32.827 kb on - strand, within RR42_RS20970 at 32.871 kb on + strand, within RR42_RS20970 at 32.872 kb on - strand, within RR42_RS20970 at 33.000 kb on + strand, within RR42_RS20970 at 33.122 kb on - strand, within RR42_RS20970 at 33.122 kb on - strand, within RR42_RS20970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 29,957 + -0.7 30,126 + +0.5 30,307 + RR42_RS20960 0.28 +2.3 30,307 + RR42_RS20960 0.28 -1.6 30,307 + RR42_RS20960 0.28 -1.0 30,307 + RR42_RS20960 0.28 -2.0 30,308 - RR42_RS20960 0.28 -0.5 30,308 - RR42_RS20960 0.28 +0.9 30,308 - RR42_RS20960 0.28 +0.1 30,354 - RR42_RS20960 0.34 -0.5 30,451 + RR42_RS20960 0.46 -0.6 30,452 - RR42_RS20960 0.46 -0.6 30,701 - RR42_RS20960 0.77 -0.6 30,751 - RR42_RS20960 0.83 +0.3 30,865 + -0.2 31,180 - RR42_RS20965 0.21 -0.6 31,438 - RR42_RS20965 0.42 +0.1 31,881 - RR42_RS20965 0.80 -0.8 31,881 - RR42_RS20965 0.80 -0.1 32,122 - +0.3 32,212 - -0.5 32,292 + -0.8 32,293 - -0.2 32,556 + RR42_RS20970 0.29 +0.5 32,676 + RR42_RS20970 0.38 +0.2 32,776 + RR42_RS20970 0.46 +0.9 32,826 + RR42_RS20970 0.50 +0.2 32,826 + RR42_RS20970 0.50 +0.1 32,827 - RR42_RS20970 0.50 -2.0 32,827 - RR42_RS20970 0.50 -0.6 32,827 - RR42_RS20970 0.50 -1.0 32,871 + RR42_RS20970 0.53 -0.4 32,872 - RR42_RS20970 0.53 +0.3 33,000 + RR42_RS20970 0.63 -1.5 33,122 - RR42_RS20970 0.73 +0.3 33,122 - RR42_RS20970 0.73 -1.9
Or see this region's nucleotide sequence