Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20700 and RR42_RS20705 are separated by 21 nucleotides RR42_RS20705 and RR42_RS20710 are separated by 161 nucleotides RR42_RS20710 and RR42_RS20715 are separated by 273 nucleotides
RR42_RS20700: RR42_RS20700 - GMC family oxidoreductase, at 4,494,897 to 4,496,492
_RS20700
RR42_RS20705: RR42_RS20705 - hypothetical protein, at 4,496,514 to 4,497,098
_RS20705
RR42_RS20710: RR42_RS20710 - AraC family transcriptional regulator, at 4,497,260 to 4,498,363
_RS20710
RR42_RS20715: RR42_RS20715 - 4-hydroxyacetophenone monooxygenase, at 4,498,637 to 4,500,211
_RS20715
Position (kb) 4497
4498
4499 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4496.473 kb on + strand at 4496.473 kb on + strand at 4496.474 kb on - strand at 4496.474 kb on - strand at 4496.474 kb on - strand at 4496.474 kb on - strand at 4496.474 kb on - strand at 4496.505 kb on + strand at 4496.590 kb on + strand, within RR42_RS20705 at 4496.590 kb on + strand, within RR42_RS20705 at 4496.590 kb on + strand, within RR42_RS20705 at 4496.591 kb on - strand, within RR42_RS20705 at 4496.591 kb on - strand, within RR42_RS20705 at 4496.593 kb on + strand, within RR42_RS20705 at 4496.594 kb on - strand, within RR42_RS20705 at 4496.847 kb on + strand, within RR42_RS20705 at 4496.847 kb on + strand, within RR42_RS20705 at 4496.848 kb on - strand, within RR42_RS20705 at 4497.320 kb on + strand at 4497.320 kb on + strand at 4497.321 kb on - strand at 4497.321 kb on - strand at 4497.321 kb on - strand at 4497.321 kb on - strand at 4497.321 kb on - strand at 4497.590 kb on + strand, within RR42_RS20710 at 4497.644 kb on + strand, within RR42_RS20710 at 4497.866 kb on + strand, within RR42_RS20710 at 4498.061 kb on - strand, within RR42_RS20710 at 4498.169 kb on + strand, within RR42_RS20710 at 4498.169 kb on + strand, within RR42_RS20710 at 4498.170 kb on - strand, within RR42_RS20710 at 4498.361 kb on + strand at 4498.384 kb on + strand at 4498.384 kb on + strand at 4498.595 kb on - strand at 4498.636 kb on + strand at 4498.636 kb on + strand at 4498.636 kb on + strand at 4498.637 kb on - strand at 4498.637 kb on - strand at 4498.637 kb on - strand at 4498.669 kb on - strand at 4498.883 kb on + strand, within RR42_RS20715 at 4498.883 kb on + strand, within RR42_RS20715 at 4498.883 kb on + strand, within RR42_RS20715 at 4498.883 kb on + strand, within RR42_RS20715 at 4498.884 kb on - strand, within RR42_RS20715 at 4498.884 kb on - strand, within RR42_RS20715 at 4499.198 kb on + strand, within RR42_RS20715 at 4499.198 kb on + strand, within RR42_RS20715
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,496,473 + -0.4 4,496,473 + -0.4 4,496,474 - -0.8 4,496,474 - -0.3 4,496,474 - -0.7 4,496,474 - +0.5 4,496,474 - -0.4 4,496,505 + +0.5 4,496,590 + RR42_RS20705 0.13 -1.6 4,496,590 + RR42_RS20705 0.13 -1.9 4,496,590 + RR42_RS20705 0.13 +1.1 4,496,591 - RR42_RS20705 0.13 -0.5 4,496,591 - RR42_RS20705 0.13 -1.1 4,496,593 + RR42_RS20705 0.14 +0.3 4,496,594 - RR42_RS20705 0.14 -0.2 4,496,847 + RR42_RS20705 0.57 +0.7 4,496,847 + RR42_RS20705 0.57 +0.6 4,496,848 - RR42_RS20705 0.57 +1.4 4,497,320 + +0.2 4,497,320 + -1.2 4,497,321 - -0.3 4,497,321 - +0.2 4,497,321 - -0.3 4,497,321 - -0.6 4,497,321 - +0.1 4,497,590 + RR42_RS20710 0.30 -3.5 4,497,644 + RR42_RS20710 0.35 -0.1 4,497,866 + RR42_RS20710 0.55 +1.2 4,498,061 - RR42_RS20710 0.73 -1.3 4,498,169 + RR42_RS20710 0.82 -0.1 4,498,169 + RR42_RS20710 0.82 -0.3 4,498,170 - RR42_RS20710 0.82 -0.9 4,498,361 + +1.2 4,498,384 + +0.1 4,498,384 + +1.0 4,498,595 - +1.1 4,498,636 + +0.2 4,498,636 + -0.9 4,498,636 + -1.3 4,498,637 - +0.2 4,498,637 - +0.4 4,498,637 - -0.1 4,498,669 - -0.2 4,498,883 + RR42_RS20715 0.16 +1.1 4,498,883 + RR42_RS20715 0.16 +1.2 4,498,883 + RR42_RS20715 0.16 +1.0 4,498,883 + RR42_RS20715 0.16 +0.2 4,498,884 - RR42_RS20715 0.16 -0.8 4,498,884 - RR42_RS20715 0.16 +0.1 4,499,198 + RR42_RS20715 0.36 +0.6 4,499,198 + RR42_RS20715 0.36 -1.8
Or see this region's nucleotide sequence