Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20025 and RR42_RS20030 are separated by 207 nucleotides RR42_RS20030 and RR42_RS20035 are separated by 30 nucleotides
RR42_RS20025: RR42_RS20025 - coproporphyrinogen III oxidase, at 4,325,092 to 4,326,693
_RS20025
RR42_RS20030: RR42_RS20030 - ABC transporter permease, at 4,326,901 to 4,328,043
_RS20030
RR42_RS20035: RR42_RS20035 - histidine kinase, at 4,328,074 to 4,329,195
_RS20035
Position (kb)
4326
4327
4328
4329 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4326.270 kb on - strand, within RR42_RS20025 at 4326.324 kb on - strand, within RR42_RS20025 at 4326.356 kb on + strand, within RR42_RS20025 at 4326.356 kb on + strand, within RR42_RS20025 at 4326.356 kb on + strand, within RR42_RS20025 at 4326.357 kb on - strand, within RR42_RS20025 at 4326.357 kb on - strand, within RR42_RS20025 at 4326.357 kb on - strand, within RR42_RS20025 at 4326.357 kb on - strand, within RR42_RS20025 at 4326.491 kb on + strand, within RR42_RS20025 at 4326.508 kb on - strand, within RR42_RS20025 at 4326.729 kb on + strand at 4327.082 kb on - strand, within RR42_RS20030 at 4327.082 kb on - strand, within RR42_RS20030 at 4327.409 kb on - strand, within RR42_RS20030 at 4327.409 kb on - strand, within RR42_RS20030 at 4327.409 kb on - strand, within RR42_RS20030 at 4327.660 kb on + strand, within RR42_RS20030 at 4327.726 kb on + strand, within RR42_RS20030 at 4327.726 kb on + strand, within RR42_RS20030 at 4327.727 kb on - strand, within RR42_RS20030 at 4327.859 kb on - strand, within RR42_RS20030 at 4327.859 kb on - strand, within RR42_RS20030 at 4327.859 kb on - strand, within RR42_RS20030 at 4328.228 kb on + strand, within RR42_RS20035 at 4328.537 kb on + strand, within RR42_RS20035 at 4328.537 kb on + strand, within RR42_RS20035 at 4328.537 kb on + strand, within RR42_RS20035 at 4328.538 kb on - strand, within RR42_RS20035 at 4328.538 kb on - strand, within RR42_RS20035 at 4328.538 kb on - strand, within RR42_RS20035 at 4328.540 kb on + strand, within RR42_RS20035 at 4328.540 kb on + strand, within RR42_RS20035 at 4328.540 kb on + strand, within RR42_RS20035 at 4328.540 kb on + strand, within RR42_RS20035 at 4328.541 kb on - strand, within RR42_RS20035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,326,270 - RR42_RS20025 0.74 -0.7 4,326,324 - RR42_RS20025 0.77 +1.0 4,326,356 + RR42_RS20025 0.79 +0.1 4,326,356 + RR42_RS20025 0.79 +0.7 4,326,356 + RR42_RS20025 0.79 +2.0 4,326,357 - RR42_RS20025 0.79 -0.9 4,326,357 - RR42_RS20025 0.79 +0.3 4,326,357 - RR42_RS20025 0.79 -0.3 4,326,357 - RR42_RS20025 0.79 +0.1 4,326,491 + RR42_RS20025 0.87 +0.2 4,326,508 - RR42_RS20025 0.88 +0.2 4,326,729 + -0.0 4,327,082 - RR42_RS20030 0.16 -0.2 4,327,082 - RR42_RS20030 0.16 -0.3 4,327,409 - RR42_RS20030 0.44 +0.8 4,327,409 - RR42_RS20030 0.44 +0.5 4,327,409 - RR42_RS20030 0.44 -0.1 4,327,660 + RR42_RS20030 0.66 +0.2 4,327,726 + RR42_RS20030 0.72 -1.6 4,327,726 + RR42_RS20030 0.72 +1.1 4,327,727 - RR42_RS20030 0.72 -0.9 4,327,859 - RR42_RS20030 0.84 -0.8 4,327,859 - RR42_RS20030 0.84 -0.5 4,327,859 - RR42_RS20030 0.84 -0.6 4,328,228 + RR42_RS20035 0.14 -1.4 4,328,537 + RR42_RS20035 0.41 -2.3 4,328,537 + RR42_RS20035 0.41 -0.8 4,328,537 + RR42_RS20035 0.41 -0.2 4,328,538 - RR42_RS20035 0.41 +0.4 4,328,538 - RR42_RS20035 0.41 -0.6 4,328,538 - RR42_RS20035 0.41 +0.4 4,328,540 + RR42_RS20035 0.42 -0.2 4,328,540 + RR42_RS20035 0.42 +1.3 4,328,540 + RR42_RS20035 0.42 -0.3 4,328,540 + RR42_RS20035 0.42 +0.4 4,328,541 - RR42_RS20035 0.42 -1.0
Or see this region's nucleotide sequence