Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19865 and RR42_RS19870 are separated by 64 nucleotides RR42_RS19870 and RR42_RS19875 are separated by 101 nucleotides
RR42_RS19865: RR42_RS19865 - hydroxypyruvate reductase, at 4,288,624 to 4,289,937
_RS19865
RR42_RS19870: RR42_RS19870 - pyruvate kinase, at 4,290,002 to 4,291,480
_RS19870
RR42_RS19875: RR42_RS19875 - metalloprotease, at 4,291,582 to 4,292,475
_RS19875
Position (kb)
4290
4291
4292 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4289.108 kb on + strand, within RR42_RS19865 at 4289.109 kb on - strand, within RR42_RS19865 at 4289.109 kb on - strand, within RR42_RS19865 at 4289.320 kb on + strand, within RR42_RS19865 at 4289.320 kb on + strand, within RR42_RS19865 at 4289.321 kb on - strand, within RR42_RS19865 at 4289.935 kb on + strand at 4290.899 kb on + strand, within RR42_RS19870 at 4290.900 kb on - strand, within RR42_RS19870 at 4290.900 kb on - strand, within RR42_RS19870 at 4290.900 kb on - strand, within RR42_RS19870 at 4291.128 kb on - strand, within RR42_RS19870 at 4291.128 kb on - strand, within RR42_RS19870 at 4291.323 kb on - strand, within RR42_RS19870 at 4291.517 kb on + strand at 4291.517 kb on + strand at 4291.517 kb on + strand at 4291.517 kb on + strand at 4291.518 kb on - strand at 4291.518 kb on - strand at 4291.518 kb on - strand at 4291.518 kb on - strand at 4291.520 kb on - strand at 4291.567 kb on + strand at 4291.567 kb on + strand at 4291.568 kb on - strand at 4291.616 kb on + strand at 4291.616 kb on + strand at 4291.616 kb on + strand at 4291.616 kb on + strand at 4291.617 kb on - strand at 4291.717 kb on + strand, within RR42_RS19875 at 4291.887 kb on + strand, within RR42_RS19875 at 4291.887 kb on + strand, within RR42_RS19875 at 4291.887 kb on + strand, within RR42_RS19875 at 4291.888 kb on - strand, within RR42_RS19875 at 4291.915 kb on + strand, within RR42_RS19875 at 4291.996 kb on + strand, within RR42_RS19875 at 4292.020 kb on + strand, within RR42_RS19875 at 4292.020 kb on + strand, within RR42_RS19875 at 4292.020 kb on + strand, within RR42_RS19875 at 4292.021 kb on - strand, within RR42_RS19875 at 4292.021 kb on - strand, within RR42_RS19875 at 4292.089 kb on + strand, within RR42_RS19875 at 4292.089 kb on + strand, within RR42_RS19875 at 4292.089 kb on + strand, within RR42_RS19875 at 4292.089 kb on + strand, within RR42_RS19875 at 4292.089 kb on + strand, within RR42_RS19875 at 4292.090 kb on - strand, within RR42_RS19875 at 4292.090 kb on - strand, within RR42_RS19875 at 4292.090 kb on - strand, within RR42_RS19875 at 4292.356 kb on + strand, within RR42_RS19875 at 4292.356 kb on + strand, within RR42_RS19875 at 4292.357 kb on - strand, within RR42_RS19875
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,289,108 + RR42_RS19865 0.37 -1.1 4,289,109 - RR42_RS19865 0.37 -2.6 4,289,109 - RR42_RS19865 0.37 +1.0 4,289,320 + RR42_RS19865 0.53 -0.7 4,289,320 + RR42_RS19865 0.53 +0.0 4,289,321 - RR42_RS19865 0.53 -1.3 4,289,935 + +0.9 4,290,899 + RR42_RS19870 0.61 -0.5 4,290,900 - RR42_RS19870 0.61 -0.8 4,290,900 - RR42_RS19870 0.61 +0.1 4,290,900 - RR42_RS19870 0.61 +0.1 4,291,128 - RR42_RS19870 0.76 +1.0 4,291,128 - RR42_RS19870 0.76 -0.5 4,291,323 - RR42_RS19870 0.89 -0.2 4,291,517 + -0.1 4,291,517 + -0.7 4,291,517 + +0.4 4,291,517 + +1.7 4,291,518 - -0.8 4,291,518 - -0.7 4,291,518 - -0.8 4,291,518 - -0.4 4,291,520 - -0.9 4,291,567 + -2.3 4,291,567 + +0.5 4,291,568 - -0.0 4,291,616 + -1.1 4,291,616 + -1.0 4,291,616 + +0.3 4,291,616 + -0.3 4,291,617 - +1.2 4,291,717 + RR42_RS19875 0.15 +0.1 4,291,887 + RR42_RS19875 0.34 -0.3 4,291,887 + RR42_RS19875 0.34 -2.6 4,291,887 + RR42_RS19875 0.34 -1.6 4,291,888 - RR42_RS19875 0.34 -0.5 4,291,915 + RR42_RS19875 0.37 -1.6 4,291,996 + RR42_RS19875 0.46 -1.6 4,292,020 + RR42_RS19875 0.49 -3.2 4,292,020 + RR42_RS19875 0.49 -1.9 4,292,020 + RR42_RS19875 0.49 -0.8 4,292,021 - RR42_RS19875 0.49 +0.5 4,292,021 - RR42_RS19875 0.49 -0.5 4,292,089 + RR42_RS19875 0.57 -1.4 4,292,089 + RR42_RS19875 0.57 +0.7 4,292,089 + RR42_RS19875 0.57 -1.6 4,292,089 + RR42_RS19875 0.57 -0.7 4,292,089 + RR42_RS19875 0.57 +0.6 4,292,090 - RR42_RS19875 0.57 -0.0 4,292,090 - RR42_RS19875 0.57 +0.1 4,292,090 - RR42_RS19875 0.57 -0.1 4,292,356 + RR42_RS19875 0.87 -1.6 4,292,356 + RR42_RS19875 0.87 -1.2 4,292,357 - RR42_RS19875 0.87 -0.3
Or see this region's nucleotide sequence