Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19725 and RR42_RS19730 are separated by 129 nucleotides RR42_RS19730 and RR42_RS19735 are separated by 98 nucleotides RR42_RS19735 and RR42_RS19740 are separated by 39 nucleotides
RR42_RS19725: RR42_RS19725 - hypothetical protein, at 4,259,690 to 4,260,478
_RS19725
RR42_RS19730: RR42_RS19730 - phosphoheptose isomerase, at 4,260,608 to 4,261,195
_RS19730
RR42_RS19735: RR42_RS19735 - hypothetical protein, at 4,261,294 to 4,261,662
_RS19735
RR42_RS19740: RR42_RS19740 - 16S rRNA methyltransferase, at 4,261,702 to 4,262,625
_RS19740
Position (kb) 4260
4261
4262 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4259.895 kb on + strand, within RR42_RS19725 at 4259.895 kb on + strand, within RR42_RS19725 at 4259.895 kb on + strand, within RR42_RS19725 at 4259.895 kb on + strand, within RR42_RS19725 at 4260.167 kb on + strand, within RR42_RS19725 at 4260.167 kb on + strand, within RR42_RS19725 at 4260.167 kb on + strand, within RR42_RS19725 at 4260.167 kb on + strand, within RR42_RS19725 at 4260.167 kb on + strand, within RR42_RS19725 at 4260.168 kb on - strand, within RR42_RS19725 at 4260.168 kb on - strand, within RR42_RS19725 at 4260.168 kb on - strand, within RR42_RS19725 at 4260.168 kb on - strand, within RR42_RS19725 at 4260.171 kb on + strand, within RR42_RS19725 at 4260.171 kb on + strand, within RR42_RS19725 at 4260.171 kb on + strand, within RR42_RS19725 at 4260.172 kb on - strand, within RR42_RS19725 at 4260.172 kb on - strand, within RR42_RS19725 at 4260.172 kb on - strand, within RR42_RS19725 at 4260.609 kb on + strand at 4260.916 kb on - strand, within RR42_RS19730 at 4261.189 kb on + strand at 4261.189 kb on + strand at 4261.189 kb on + strand at 4261.190 kb on - strand at 4261.190 kb on - strand at 4261.235 kb on - strand at 4261.263 kb on + strand at 4261.478 kb on + strand, within RR42_RS19735 at 4261.478 kb on + strand, within RR42_RS19735 at 4261.479 kb on - strand, within RR42_RS19735 at 4261.479 kb on - strand, within RR42_RS19735 at 4261.479 kb on - strand, within RR42_RS19735 at 4261.572 kb on - strand, within RR42_RS19735 at 4261.756 kb on + strand at 4261.756 kb on + strand at 4261.756 kb on + strand at 4261.756 kb on + strand at 4261.756 kb on + strand at 4261.757 kb on - strand at 4261.757 kb on - strand at 4261.757 kb on - strand at 4261.757 kb on - strand at 4261.815 kb on - strand, within RR42_RS19740 at 4261.917 kb on + strand, within RR42_RS19740 at 4261.979 kb on - strand, within RR42_RS19740 at 4261.979 kb on - strand, within RR42_RS19740 at 4261.979 kb on - strand, within RR42_RS19740 at 4261.979 kb on - strand, within RR42_RS19740 at 4261.979 kb on - strand, within RR42_RS19740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,259,895 + RR42_RS19725 0.26 +0.2 4,259,895 + RR42_RS19725 0.26 -0.3 4,259,895 + RR42_RS19725 0.26 -0.6 4,259,895 + RR42_RS19725 0.26 +0.2 4,260,167 + RR42_RS19725 0.60 -0.8 4,260,167 + RR42_RS19725 0.60 -0.1 4,260,167 + RR42_RS19725 0.60 -0.6 4,260,167 + RR42_RS19725 0.60 +0.7 4,260,167 + RR42_RS19725 0.60 +3.8 4,260,168 - RR42_RS19725 0.61 +2.2 4,260,168 - RR42_RS19725 0.61 -1.0 4,260,168 - RR42_RS19725 0.61 -1.0 4,260,168 - RR42_RS19725 0.61 +0.7 4,260,171 + RR42_RS19725 0.61 -0.4 4,260,171 + RR42_RS19725 0.61 -0.4 4,260,171 + RR42_RS19725 0.61 -0.1 4,260,172 - RR42_RS19725 0.61 -0.9 4,260,172 - RR42_RS19725 0.61 +1.4 4,260,172 - RR42_RS19725 0.61 +0.4 4,260,609 + +0.4 4,260,916 - RR42_RS19730 0.52 +0.5 4,261,189 + -0.4 4,261,189 + -0.0 4,261,189 + -0.7 4,261,190 - -1.6 4,261,190 - -1.9 4,261,235 - -0.4 4,261,263 + -0.4 4,261,478 + RR42_RS19735 0.50 +0.0 4,261,478 + RR42_RS19735 0.50 -0.4 4,261,479 - RR42_RS19735 0.50 +0.7 4,261,479 - RR42_RS19735 0.50 +1.0 4,261,479 - RR42_RS19735 0.50 -0.7 4,261,572 - RR42_RS19735 0.75 +0.2 4,261,756 + -0.6 4,261,756 + -0.2 4,261,756 + -0.9 4,261,756 + -1.3 4,261,756 + +1.4 4,261,757 - -1.1 4,261,757 - +1.0 4,261,757 - +0.9 4,261,757 - -0.1 4,261,815 - RR42_RS19740 0.12 +0.2 4,261,917 + RR42_RS19740 0.23 -1.3 4,261,979 - RR42_RS19740 0.30 -1.3 4,261,979 - RR42_RS19740 0.30 -1.6 4,261,979 - RR42_RS19740 0.30 +0.3 4,261,979 - RR42_RS19740 0.30 -0.3 4,261,979 - RR42_RS19740 0.30 -0.5
Or see this region's nucleotide sequence