Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19510 and RR42_RS19515 overlap by 1 nucleotides RR42_RS19515 and RR42_RS19520 are separated by 53 nucleotides RR42_RS19520 and RR42_RS19525 are separated by 8 nucleotides
RR42_RS19510: RR42_RS19510 - general secretion pathway protein GspM, at 4,212,168 to 4,212,794
_RS19510
RR42_RS19515: RR42_RS19515 - general secretion pathway protein GspN, at 4,212,794 to 4,213,633
_RS19515
RR42_RS19520: RR42_RS19520 - general secretion pathway protein GspD, at 4,213,687 to 4,216,116
_RS19520
RR42_RS19525: RR42_RS19525 - general secretion pathway protein GspE, at 4,216,125 to 4,217,672
_RS19525
Position (kb)
4213
4214
4215
4216
4217 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7
8
9
10 at 4212.717 kb on + strand, within RR42_RS19510 at 4212.718 kb on - strand, within RR42_RS19510 at 4212.718 kb on - strand, within RR42_RS19510 at 4212.718 kb on - strand, within RR42_RS19510 at 4213.246 kb on + strand, within RR42_RS19515 at 4213.369 kb on - strand, within RR42_RS19515 at 4213.383 kb on + strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.384 kb on - strand, within RR42_RS19515 at 4213.471 kb on + strand, within RR42_RS19515 at 4213.471 kb on + strand, within RR42_RS19515 at 4213.472 kb on - strand, within RR42_RS19515 at 4213.472 kb on - strand, within RR42_RS19515 at 4213.585 kb on - strand at 4213.585 kb on - strand at 4213.687 kb on - strand at 4213.748 kb on + strand at 4213.748 kb on + strand at 4214.038 kb on + strand, within RR42_RS19520 at 4214.039 kb on - strand, within RR42_RS19520 at 4214.039 kb on - strand, within RR42_RS19520 at 4214.039 kb on - strand, within RR42_RS19520 at 4214.072 kb on + strand, within RR42_RS19520 at 4214.072 kb on + strand, within RR42_RS19520 at 4214.072 kb on + strand, within RR42_RS19520 at 4214.073 kb on - strand, within RR42_RS19520 at 4214.073 kb on - strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.173 kb on + strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.174 kb on - strand, within RR42_RS19520 at 4214.275 kb on + strand, within RR42_RS19520 at 4214.275 kb on + strand, within RR42_RS19520 at 4214.276 kb on - strand, within RR42_RS19520 at 4214.276 kb on - strand, within RR42_RS19520 at 4214.276 kb on - strand, within RR42_RS19520 at 4214.276 kb on - strand, within RR42_RS19520 at 4214.572 kb on + strand, within RR42_RS19520 at 4214.573 kb on - strand, within RR42_RS19520 at 4214.589 kb on + strand, within RR42_RS19520 at 4214.589 kb on + strand, within RR42_RS19520 at 4214.590 kb on - strand, within RR42_RS19520 at 4214.590 kb on - strand, within RR42_RS19520 at 4214.590 kb on - strand, within RR42_RS19520 at 4214.760 kb on + strand, within RR42_RS19520 at 4214.770 kb on + strand, within RR42_RS19520 at 4214.771 kb on - strand, within RR42_RS19520 at 4214.771 kb on - strand, within RR42_RS19520 at 4214.771 kb on - strand, within RR42_RS19520 at 4214.936 kb on - strand, within RR42_RS19520 at 4214.989 kb on + strand, within RR42_RS19520 at 4214.989 kb on + strand, within RR42_RS19520 at 4214.989 kb on + strand, within RR42_RS19520 at 4214.989 kb on + strand, within RR42_RS19520 at 4214.990 kb on - strand, within RR42_RS19520 at 4214.990 kb on - strand, within RR42_RS19520 at 4214.990 kb on - strand, within RR42_RS19520 at 4215.386 kb on - strand, within RR42_RS19520 at 4215.647 kb on - strand, within RR42_RS19520 at 4215.647 kb on - strand, within RR42_RS19520 at 4215.763 kb on + strand, within RR42_RS19520 at 4215.764 kb on - strand, within RR42_RS19520 at 4215.764 kb on - strand, within RR42_RS19520 at 4215.812 kb on - strand, within RR42_RS19520 at 4215.818 kb on - strand, within RR42_RS19520 at 4215.819 kb on + strand, within RR42_RS19520 at 4215.819 kb on + strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.820 kb on - strand, within RR42_RS19520 at 4215.855 kb on + strand, within RR42_RS19520 at 4215.877 kb on - strand at 4216.216 kb on - strand at 4216.217 kb on - strand at 4216.392 kb on + strand, within RR42_RS19525 at 4216.392 kb on + strand, within RR42_RS19525 at 4216.978 kb on - strand, within RR42_RS19525 at 4217.043 kb on + strand, within RR42_RS19525
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,212,717 + RR42_RS19510 0.88 +7.2 4,212,718 - RR42_RS19510 0.88 +7.2 4,212,718 - RR42_RS19510 0.88 +7.0 4,212,718 - RR42_RS19510 0.88 +6.6 4,213,246 + RR42_RS19515 0.54 +6.9 4,213,369 - RR42_RS19515 0.68 +6.7 4,213,383 + RR42_RS19515 0.70 +6.2 4,213,384 - RR42_RS19515 0.70 +7.7 4,213,384 - RR42_RS19515 0.70 +7.3 4,213,384 - RR42_RS19515 0.70 +6.8 4,213,384 - RR42_RS19515 0.70 +7.3 4,213,384 - RR42_RS19515 0.70 +7.1 4,213,471 + RR42_RS19515 0.81 +5.8 4,213,471 + RR42_RS19515 0.81 +6.9 4,213,472 - RR42_RS19515 0.81 +7.0 4,213,472 - RR42_RS19515 0.81 +7.0 4,213,585 - +7.6 4,213,585 - +6.4 4,213,687 - +7.3 4,213,748 + +7.3 4,213,748 + +7.3 4,214,038 + RR42_RS19520 0.14 +4.0 4,214,039 - RR42_RS19520 0.14 +7.2 4,214,039 - RR42_RS19520 0.14 +7.0 4,214,039 - RR42_RS19520 0.14 +8.2 4,214,072 + RR42_RS19520 0.16 +7.9 4,214,072 + RR42_RS19520 0.16 +8.2 4,214,072 + RR42_RS19520 0.16 +7.7 4,214,073 - RR42_RS19520 0.16 +6.8 4,214,073 - RR42_RS19520 0.16 +10.0 4,214,173 + RR42_RS19520 0.20 +7.5 4,214,173 + RR42_RS19520 0.20 +6.8 4,214,173 + RR42_RS19520 0.20 +7.2 4,214,173 + RR42_RS19520 0.20 +7.6 4,214,173 + RR42_RS19520 0.20 +7.0 4,214,173 + RR42_RS19520 0.20 +7.5 4,214,173 + RR42_RS19520 0.20 +7.1 4,214,174 - RR42_RS19520 0.20 +7.5 4,214,174 - RR42_RS19520 0.20 +7.6 4,214,174 - RR42_RS19520 0.20 +6.8 4,214,174 - RR42_RS19520 0.20 +7.0 4,214,174 - RR42_RS19520 0.20 +7.2 4,214,275 + RR42_RS19520 0.24 +7.7 4,214,275 + RR42_RS19520 0.24 +6.9 4,214,276 - RR42_RS19520 0.24 +7.5 4,214,276 - RR42_RS19520 0.24 +7.0 4,214,276 - RR42_RS19520 0.24 +6.7 4,214,276 - RR42_RS19520 0.24 +7.4 4,214,572 + RR42_RS19520 0.36 +6.5 4,214,573 - RR42_RS19520 0.36 +6.7 4,214,589 + RR42_RS19520 0.37 +6.8 4,214,589 + RR42_RS19520 0.37 +7.1 4,214,590 - RR42_RS19520 0.37 +6.9 4,214,590 - RR42_RS19520 0.37 +7.4 4,214,590 - RR42_RS19520 0.37 +4.6 4,214,760 + RR42_RS19520 0.44 +7.2 4,214,770 + RR42_RS19520 0.45 +7.3 4,214,771 - RR42_RS19520 0.45 +7.5 4,214,771 - RR42_RS19520 0.45 +7.8 4,214,771 - RR42_RS19520 0.45 +7.5 4,214,936 - RR42_RS19520 0.51 +7.6 4,214,989 + RR42_RS19520 0.54 +7.0 4,214,989 + RR42_RS19520 0.54 +7.8 4,214,989 + RR42_RS19520 0.54 +8.0 4,214,989 + RR42_RS19520 0.54 +7.0 4,214,990 - RR42_RS19520 0.54 +7.5 4,214,990 - RR42_RS19520 0.54 +6.8 4,214,990 - RR42_RS19520 0.54 +7.4 4,215,386 - RR42_RS19520 0.70 +7.5 4,215,647 - RR42_RS19520 0.81 +7.2 4,215,647 - RR42_RS19520 0.81 +7.1 4,215,763 + RR42_RS19520 0.85 +6.8 4,215,764 - RR42_RS19520 0.85 +7.8 4,215,764 - RR42_RS19520 0.85 +8.0 4,215,812 - RR42_RS19520 0.87 +6.8 4,215,818 - RR42_RS19520 0.88 +7.7 4,215,819 + RR42_RS19520 0.88 +6.9 4,215,819 + RR42_RS19520 0.88 +7.1 4,215,820 - RR42_RS19520 0.88 +7.0 4,215,820 - RR42_RS19520 0.88 +7.3 4,215,820 - RR42_RS19520 0.88 +6.8 4,215,820 - RR42_RS19520 0.88 +7.7 4,215,820 - RR42_RS19520 0.88 +6.9 4,215,855 + RR42_RS19520 0.89 +7.1 4,215,877 - +6.9 4,216,216 - +5.5 4,216,217 - +6.9 4,216,392 + RR42_RS19525 0.17 +7.9 4,216,392 + RR42_RS19525 0.17 +6.8 4,216,978 - RR42_RS19525 0.55 +7.1 4,217,043 + RR42_RS19525 0.59 +7.2
Or see this region's nucleotide sequence