Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19255 and RR42_RS19260 are separated by 236 nucleotides RR42_RS19260 and RR42_RS19265 are separated by 1 nucleotides RR42_RS19265 and RR42_RS19270 are separated by 71 nucleotides RR42_RS19270 and RR42_RS19275 are separated by 112 nucleotides
RR42_RS19255: RR42_RS19255 - 50S ribosomal protein L3, at 4,157,521 to 4,158,171
_RS19255
RR42_RS19260: RR42_RS19260 - nitrilotriacetate monooxygenase, at 4,158,408 to 4,158,899
_RS19260
RR42_RS19265: RR42_RS19265 - alanine-phosphoribitol ligase, at 4,158,901 to 4,160,142
_RS19265
RR42_RS19270: RR42_RS19270 - short-chain dehydrogenase, at 4,160,214 to 4,161,002
_RS19270
RR42_RS19275: RR42_RS19275 - carboxymethylenebutenolidase, at 4,161,115 to 4,162,344
_RS19275
Position (kb) 4158
4159
4160
4161 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4158.358 kb on + strand at 4158.358 kb on + strand at 4158.358 kb on + strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.409 kb on + strand at 4158.565 kb on + strand, within RR42_RS19260 at 4158.565 kb on + strand, within RR42_RS19260 at 4158.566 kb on - strand, within RR42_RS19260 at 4158.566 kb on - strand, within RR42_RS19260 at 4159.118 kb on + strand, within RR42_RS19265 at 4159.118 kb on + strand, within RR42_RS19265 at 4159.119 kb on - strand, within RR42_RS19265 at 4159.249 kb on + strand, within RR42_RS19265 at 4159.250 kb on - strand, within RR42_RS19265 at 4159.250 kb on - strand, within RR42_RS19265 at 4159.402 kb on - strand, within RR42_RS19265 at 4159.402 kb on - strand, within RR42_RS19265 at 4159.402 kb on - strand, within RR42_RS19265 at 4159.402 kb on - strand, within RR42_RS19265 at 4159.444 kb on - strand, within RR42_RS19265 at 4159.541 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.924 kb on + strand, within RR42_RS19265 at 4159.924 kb on + strand, within RR42_RS19265 at 4159.924 kb on + strand, within RR42_RS19265 at 4159.925 kb on - strand, within RR42_RS19265 at 4159.925 kb on - strand, within RR42_RS19265 at 4159.938 kb on + strand, within RR42_RS19265 at 4159.938 kb on + strand, within RR42_RS19265 at 4159.939 kb on - strand, within RR42_RS19265 at 4159.939 kb on - strand, within RR42_RS19265 at 4159.939 kb on - strand, within RR42_RS19265 at 4159.939 kb on - strand, within RR42_RS19265 at 4159.939 kb on - strand, within RR42_RS19265 at 4159.939 kb on - strand, within RR42_RS19265 at 4159.939 kb on - strand, within RR42_RS19265 at 4159.939 kb on - strand, within RR42_RS19265 at 4160.066 kb on + strand at 4160.067 kb on - strand at 4160.067 kb on - strand at 4160.153 kb on + strand at 4160.153 kb on + strand at 4160.153 kb on + strand at 4160.154 kb on - strand at 4160.154 kb on - strand at 4160.154 kb on - strand at 4160.154 kb on - strand at 4160.156 kb on + strand at 4160.156 kb on + strand at 4160.156 kb on + strand at 4160.156 kb on + strand at 4160.156 kb on + strand at 4160.156 kb on + strand at 4160.157 kb on - strand at 4160.157 kb on - strand at 4160.157 kb on - strand at 4160.157 kb on - strand at 4160.157 kb on - strand at 4160.157 kb on - strand at 4160.159 kb on + strand at 4160.159 kb on + strand at 4160.159 kb on + strand at 4160.159 kb on + strand at 4160.159 kb on + strand at 4160.159 kb on + strand at 4160.160 kb on - strand at 4160.160 kb on - strand at 4160.160 kb on - strand at 4160.160 kb on - strand at 4160.160 kb on - strand at 4160.160 kb on - strand at 4160.160 kb on - strand at 4160.270 kb on - strand at 4160.287 kb on + strand at 4160.288 kb on - strand at 4160.288 kb on - strand at 4160.288 kb on - strand at 4160.288 kb on - strand at 4160.519 kb on + strand, within RR42_RS19270 at 4160.520 kb on - strand, within RR42_RS19270 at 4160.520 kb on - strand, within RR42_RS19270 at 4160.551 kb on + strand, within RR42_RS19270 at 4160.588 kb on + strand, within RR42_RS19270 at 4160.588 kb on + strand, within RR42_RS19270 at 4160.588 kb on + strand, within RR42_RS19270 at 4160.588 kb on + strand, within RR42_RS19270 at 4160.588 kb on + strand, within RR42_RS19270 at 4160.589 kb on - strand, within RR42_RS19270 at 4160.589 kb on - strand, within RR42_RS19270 at 4160.589 kb on - strand, within RR42_RS19270 at 4160.731 kb on + strand, within RR42_RS19270 at 4160.731 kb on + strand, within RR42_RS19270 at 4160.731 kb on + strand, within RR42_RS19270 at 4160.731 kb on + strand, within RR42_RS19270 at 4160.731 kb on + strand, within RR42_RS19270 at 4160.732 kb on - strand, within RR42_RS19270 at 4160.755 kb on + strand, within RR42_RS19270 at 4160.755 kb on + strand, within RR42_RS19270 at 4160.756 kb on - strand, within RR42_RS19270 at 4160.756 kb on - strand, within RR42_RS19270 at 4160.756 kb on - strand, within RR42_RS19270 at 4160.828 kb on + strand, within RR42_RS19270 at 4161.086 kb on + strand at 4161.086 kb on + strand at 4161.086 kb on + strand at 4161.087 kb on - strand at 4161.087 kb on - strand at 4161.087 kb on - strand at 4161.096 kb on + strand at 4161.096 kb on + strand at 4161.096 kb on + strand at 4161.097 kb on - strand at 4161.097 kb on - strand at 4161.116 kb on + strand at 4161.116 kb on + strand at 4161.116 kb on + strand at 4161.116 kb on + strand at 4161.116 kb on + strand at 4161.116 kb on + strand at 4161.116 kb on + strand at 4161.117 kb on - strand at 4161.117 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,158,358 + -0.9 4,158,358 + -1.2 4,158,358 + -0.2 4,158,359 - +0.0 4,158,359 - +1.2 4,158,359 - +0.1 4,158,359 - +0.8 4,158,409 + +0.9 4,158,565 + RR42_RS19260 0.32 +0.6 4,158,565 + RR42_RS19260 0.32 +1.2 4,158,566 - RR42_RS19260 0.32 -0.2 4,158,566 - RR42_RS19260 0.32 +0.3 4,159,118 + RR42_RS19265 0.17 -0.2 4,159,118 + RR42_RS19265 0.17 -0.3 4,159,119 - RR42_RS19265 0.18 -0.8 4,159,249 + RR42_RS19265 0.28 -1.1 4,159,250 - RR42_RS19265 0.28 +0.9 4,159,250 - RR42_RS19265 0.28 +0.1 4,159,402 - RR42_RS19265 0.40 -0.1 4,159,402 - RR42_RS19265 0.40 -1.2 4,159,402 - RR42_RS19265 0.40 -0.2 4,159,402 - RR42_RS19265 0.40 -0.3 4,159,444 - RR42_RS19265 0.44 -1.1 4,159,541 + RR42_RS19265 0.52 +0.2 4,159,613 + RR42_RS19265 0.57 +0.3 4,159,613 + RR42_RS19265 0.57 +1.8 4,159,613 + RR42_RS19265 0.57 -0.3 4,159,613 + RR42_RS19265 0.57 -0.9 4,159,613 + RR42_RS19265 0.57 -0.9 4,159,613 + RR42_RS19265 0.57 -0.1 4,159,613 + RR42_RS19265 0.57 +0.6 4,159,614 - RR42_RS19265 0.57 +1.5 4,159,614 - RR42_RS19265 0.57 +0.9 4,159,614 - RR42_RS19265 0.57 -0.4 4,159,614 - RR42_RS19265 0.57 -0.0 4,159,614 - RR42_RS19265 0.57 +0.5 4,159,614 - RR42_RS19265 0.57 -0.2 4,159,614 - RR42_RS19265 0.57 -2.5 4,159,924 + RR42_RS19265 0.82 -1.4 4,159,924 + RR42_RS19265 0.82 -0.3 4,159,924 + RR42_RS19265 0.82 -0.2 4,159,925 - RR42_RS19265 0.82 -0.4 4,159,925 - RR42_RS19265 0.82 +0.2 4,159,938 + RR42_RS19265 0.83 -0.6 4,159,938 + RR42_RS19265 0.83 -0.8 4,159,939 - RR42_RS19265 0.84 +0.7 4,159,939 - RR42_RS19265 0.84 +0.2 4,159,939 - RR42_RS19265 0.84 -1.2 4,159,939 - RR42_RS19265 0.84 -0.4 4,159,939 - RR42_RS19265 0.84 +1.2 4,159,939 - RR42_RS19265 0.84 -0.8 4,159,939 - RR42_RS19265 0.84 -1.1 4,159,939 - RR42_RS19265 0.84 -1.9 4,160,066 + -0.2 4,160,067 - -0.2 4,160,067 - +1.2 4,160,153 + +0.0 4,160,153 + +0.4 4,160,153 + +0.6 4,160,154 - +0.4 4,160,154 - -2.1 4,160,154 - -1.2 4,160,154 - -0.0 4,160,156 + +2.5 4,160,156 + +0.1 4,160,156 + -0.1 4,160,156 + -0.7 4,160,156 + -0.7 4,160,156 + +1.2 4,160,157 - +0.4 4,160,157 - -0.1 4,160,157 - +0.5 4,160,157 - +0.0 4,160,157 - -0.6 4,160,157 - -0.2 4,160,159 + +0.4 4,160,159 + -0.6 4,160,159 + -1.0 4,160,159 + -0.4 4,160,159 + -0.6 4,160,159 + +1.1 4,160,160 - -0.4 4,160,160 - +1.1 4,160,160 - -0.9 4,160,160 - +0.1 4,160,160 - +0.4 4,160,160 - -1.7 4,160,160 - +0.2 4,160,270 - -0.2 4,160,287 + +0.1 4,160,288 - -0.3 4,160,288 - -2.1 4,160,288 - -0.3 4,160,288 - +0.8 4,160,519 + RR42_RS19270 0.39 +1.8 4,160,520 - RR42_RS19270 0.39 -0.1 4,160,520 - RR42_RS19270 0.39 +0.9 4,160,551 + RR42_RS19270 0.43 -1.4 4,160,588 + RR42_RS19270 0.47 +1.0 4,160,588 + RR42_RS19270 0.47 +1.2 4,160,588 + RR42_RS19270 0.47 +0.3 4,160,588 + RR42_RS19270 0.47 +0.6 4,160,588 + RR42_RS19270 0.47 +0.2 4,160,589 - RR42_RS19270 0.48 -0.8 4,160,589 - RR42_RS19270 0.48 +0.1 4,160,589 - RR42_RS19270 0.48 +0.5 4,160,731 + RR42_RS19270 0.66 +1.2 4,160,731 + RR42_RS19270 0.66 -0.2 4,160,731 + RR42_RS19270 0.66 +0.6 4,160,731 + RR42_RS19270 0.66 +2.0 4,160,731 + RR42_RS19270 0.66 -0.1 4,160,732 - RR42_RS19270 0.66 +0.8 4,160,755 + RR42_RS19270 0.69 +0.6 4,160,755 + RR42_RS19270 0.69 +0.1 4,160,756 - RR42_RS19270 0.69 +0.0 4,160,756 - RR42_RS19270 0.69 -0.7 4,160,756 - RR42_RS19270 0.69 +1.1 4,160,828 + RR42_RS19270 0.78 +0.1 4,161,086 + -0.8 4,161,086 + +0.2 4,161,086 + -0.4 4,161,087 - +0.8 4,161,087 - -1.1 4,161,087 - +0.2 4,161,096 + +0.1 4,161,096 + +0.2 4,161,096 + -2.9 4,161,097 - +0.4 4,161,097 - +0.0 4,161,116 + +0.1 4,161,116 + -2.2 4,161,116 + -0.7 4,161,116 + -0.3 4,161,116 + -0.6 4,161,116 + +0.8 4,161,116 + +0.3 4,161,117 - +2.2 4,161,117 - +0.5
Or see this region's nucleotide sequence