Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19240 and RR42_RS19245 are separated by 2 nucleotides RR42_RS19245 and RR42_RS19250 overlap by 4 nucleotides RR42_RS19250 and RR42_RS19255 are separated by 11 nucleotides RR42_RS19255 and RR42_RS19260 are separated by 236 nucleotides RR42_RS19260 and RR42_RS19265 are separated by 1 nucleotides
RR42_RS19240: RR42_RS19240 - 50S ribosomal protein L2, at 4,155,745 to 4,156,575
_RS19240
RR42_RS19245: RR42_RS19245 - 50S ribosomal protein L23, at 4,156,578 to 4,156,892
_RS19245
RR42_RS19250: RR42_RS19250 - 50S ribosomal protein L4, at 4,156,889 to 4,157,509
_RS19250
RR42_RS19255: RR42_RS19255 - 50S ribosomal protein L3, at 4,157,521 to 4,158,171
_RS19255
RR42_RS19260: RR42_RS19260 - nitrilotriacetate monooxygenase, at 4,158,408 to 4,158,899
_RS19260
RR42_RS19265: RR42_RS19265 - alanine-phosphoribitol ligase, at 4,158,901 to 4,160,142
_RS19265
Position (kb) 4157
4158
4159 Strain fitness (log2 ratio) -1
0
1 at 4158.358 kb on + strand at 4158.358 kb on + strand at 4158.358 kb on + strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.409 kb on + strand at 4158.565 kb on + strand, within RR42_RS19260 at 4158.565 kb on + strand, within RR42_RS19260 at 4158.566 kb on - strand, within RR42_RS19260 at 4158.566 kb on - strand, within RR42_RS19260 at 4159.118 kb on + strand, within RR42_RS19265 at 4159.118 kb on + strand, within RR42_RS19265 at 4159.119 kb on - strand, within RR42_RS19265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,158,358 + -0.9 4,158,358 + -1.2 4,158,358 + -0.2 4,158,359 - +0.0 4,158,359 - +1.2 4,158,359 - +0.1 4,158,359 - +0.8 4,158,409 + +0.9 4,158,565 + RR42_RS19260 0.32 +0.6 4,158,565 + RR42_RS19260 0.32 +1.2 4,158,566 - RR42_RS19260 0.32 -0.2 4,158,566 - RR42_RS19260 0.32 +0.3 4,159,118 + RR42_RS19265 0.17 -0.2 4,159,118 + RR42_RS19265 0.17 -0.3 4,159,119 - RR42_RS19265 0.18 -0.8
Or see this region's nucleotide sequence