Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19000 and RR42_RS19005 are separated by 74 nucleotides RR42_RS19005 and RR42_RS19010 overlap by 14 nucleotides RR42_RS19010 and RR42_RS19015 are separated by 246 nucleotides
RR42_RS19000: RR42_RS19000 - deoxyguanosinetriphosphate triphosphohydrolase, at 4,115,753 to 4,116,889
_RS19000
RR42_RS19005: RR42_RS19005 - 3-dehydroquinate synthase, at 4,116,964 to 4,118,070
_RS19005
RR42_RS19010: RR42_RS19010 - shikimate kinase, at 4,118,057 to 4,118,671
_RS19010
RR42_RS19015: RR42_RS19015 - secretin, at 4,118,918 to 4,121,092
_RS19015
Position (kb)
4116
4117
4118
4119 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4115.964 kb on + strand, within RR42_RS19000 at 4115.965 kb on - strand, within RR42_RS19000 at 4115.974 kb on - strand, within RR42_RS19000 at 4116.070 kb on + strand, within RR42_RS19000 at 4116.149 kb on + strand, within RR42_RS19000 at 4116.297 kb on + strand, within RR42_RS19000 at 4116.298 kb on - strand, within RR42_RS19000 at 4116.298 kb on - strand, within RR42_RS19000 at 4116.492 kb on - strand, within RR42_RS19000 at 4116.492 kb on - strand, within RR42_RS19000 at 4116.735 kb on + strand, within RR42_RS19000 at 4116.735 kb on + strand, within RR42_RS19000 at 4116.735 kb on + strand, within RR42_RS19000 at 4116.736 kb on - strand, within RR42_RS19000 at 4116.736 kb on - strand, within RR42_RS19000 at 4116.736 kb on - strand, within RR42_RS19000 at 4116.858 kb on + strand at 4116.859 kb on - strand at 4117.015 kb on - strand at 4117.341 kb on + strand, within RR42_RS19005 at 4117.341 kb on + strand, within RR42_RS19005 at 4117.342 kb on - strand, within RR42_RS19005 at 4117.718 kb on + strand, within RR42_RS19005 at 4117.718 kb on + strand, within RR42_RS19005 at 4117.718 kb on + strand, within RR42_RS19005 at 4117.719 kb on - strand, within RR42_RS19005 at 4117.926 kb on - strand, within RR42_RS19005 at 4118.033 kb on + strand at 4118.179 kb on + strand, within RR42_RS19010 at 4118.179 kb on + strand, within RR42_RS19010 at 4118.179 kb on + strand, within RR42_RS19010 at 4118.179 kb on + strand, within RR42_RS19010 at 4118.179 kb on + strand, within RR42_RS19010 at 4118.180 kb on - strand, within RR42_RS19010 at 4118.182 kb on + strand, within RR42_RS19010 at 4118.182 kb on + strand, within RR42_RS19010 at 4118.183 kb on - strand, within RR42_RS19010 at 4118.183 kb on - strand, within RR42_RS19010 at 4118.183 kb on - strand, within RR42_RS19010 at 4118.262 kb on + strand, within RR42_RS19010 at 4118.262 kb on + strand, within RR42_RS19010 at 4118.262 kb on + strand, within RR42_RS19010 at 4118.263 kb on - strand, within RR42_RS19010 at 4118.263 kb on - strand, within RR42_RS19010 at 4118.365 kb on - strand, within RR42_RS19010 at 4118.531 kb on - strand, within RR42_RS19010 at 4118.580 kb on + strand, within RR42_RS19010 at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.736 kb on + strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.737 kb on - strand at 4118.857 kb on + strand at 4118.857 kb on + strand at 4118.894 kb on + strand at 4118.895 kb on - strand at 4118.895 kb on - strand at 4118.895 kb on - strand at 4118.895 kb on - strand at 4118.920 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 4,115,964 + RR42_RS19000 0.19 +0.2 4,115,965 - RR42_RS19000 0.19 -0.7 4,115,974 - RR42_RS19000 0.19 -0.2 4,116,070 + RR42_RS19000 0.28 -0.3 4,116,149 + RR42_RS19000 0.35 -0.8 4,116,297 + RR42_RS19000 0.48 -1.1 4,116,298 - RR42_RS19000 0.48 -0.4 4,116,298 - RR42_RS19000 0.48 -0.2 4,116,492 - RR42_RS19000 0.65 +0.4 4,116,492 - RR42_RS19000 0.65 -0.8 4,116,735 + RR42_RS19000 0.86 +1.4 4,116,735 + RR42_RS19000 0.86 -0.4 4,116,735 + RR42_RS19000 0.86 +0.3 4,116,736 - RR42_RS19000 0.86 +0.2 4,116,736 - RR42_RS19000 0.86 -0.8 4,116,736 - RR42_RS19000 0.86 +0.3 4,116,858 + -2.3 4,116,859 - +0.6 4,117,015 - +0.4 4,117,341 + RR42_RS19005 0.34 -1.1 4,117,341 + RR42_RS19005 0.34 -1.7 4,117,342 - RR42_RS19005 0.34 -0.1 4,117,718 + RR42_RS19005 0.68 -0.8 4,117,718 + RR42_RS19005 0.68 +1.2 4,117,718 + RR42_RS19005 0.68 -1.7 4,117,719 - RR42_RS19005 0.68 -0.5 4,117,926 - RR42_RS19005 0.87 -0.8 4,118,033 + +1.2 4,118,179 + RR42_RS19010 0.20 -0.5 4,118,179 + RR42_RS19010 0.20 +0.9 4,118,179 + RR42_RS19010 0.20 -0.1 4,118,179 + RR42_RS19010 0.20 +1.2 4,118,179 + RR42_RS19010 0.20 -1.1 4,118,180 - RR42_RS19010 0.20 -1.3 4,118,182 + RR42_RS19010 0.20 +0.3 4,118,182 + RR42_RS19010 0.20 -0.3 4,118,183 - RR42_RS19010 0.20 -0.0 4,118,183 - RR42_RS19010 0.20 -0.5 4,118,183 - RR42_RS19010 0.20 -0.8 4,118,262 + RR42_RS19010 0.33 -1.0 4,118,262 + RR42_RS19010 0.33 +0.6 4,118,262 + RR42_RS19010 0.33 -1.1 4,118,263 - RR42_RS19010 0.33 +0.2 4,118,263 - RR42_RS19010 0.33 -0.8 4,118,365 - RR42_RS19010 0.50 -1.0 4,118,531 - RR42_RS19010 0.77 -0.7 4,118,580 + RR42_RS19010 0.85 +0.1 4,118,736 + -0.7 4,118,736 + -0.1 4,118,736 + +0.0 4,118,736 + -0.0 4,118,736 + -0.3 4,118,736 + -0.2 4,118,736 + -3.7 4,118,736 + -0.6 4,118,736 + -0.8 4,118,737 - +0.5 4,118,737 - +0.7 4,118,737 - -0.2 4,118,737 - -0.1 4,118,737 - -1.0 4,118,737 - -2.1 4,118,737 - -1.2 4,118,737 - +0.4 4,118,737 - +0.6 4,118,737 - -0.1 4,118,857 + +1.4 4,118,857 + -1.8 4,118,894 + +0.1 4,118,895 - -0.3 4,118,895 - -0.8 4,118,895 - +0.5 4,118,895 - +1.4 4,118,920 - +0.4
Or see this region's nucleotide sequence