Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17615 and RR42_RS17620 are separated by 105 nucleotides RR42_RS17620 and RR42_RS17625 are separated by 38 nucleotides RR42_RS17625 and RR42_RS17630 are separated by 47 nucleotides
RR42_RS17615: RR42_RS17615 - thiamine monophosphate kinase, at 3,812,301 to 3,813,281
_RS17615
RR42_RS17620: RR42_RS17620 - phosphatidylglycerophosphatase, at 3,813,387 to 3,813,953
_RS17620
RR42_RS17625: RR42_RS17625 - damage-inducible protein CinA, at 3,813,992 to 3,814,489
_RS17625
RR42_RS17630: RR42_RS17630 - orotidine 5'-phosphate decarboxylase, at 3,814,537 to 3,815,355
_RS17630
Position (kb)
3813
3814 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3813.411 kb on - strand at 3813.411 kb on - strand at 3813.567 kb on + strand, within RR42_RS17620 at 3813.567 kb on + strand, within RR42_RS17620 at 3813.568 kb on - strand, within RR42_RS17620 at 3813.582 kb on + strand, within RR42_RS17620 at 3813.582 kb on + strand, within RR42_RS17620 at 3813.583 kb on - strand, within RR42_RS17620 at 3813.635 kb on - strand, within RR42_RS17620 at 3813.849 kb on - strand, within RR42_RS17620 at 3813.915 kb on + strand at 3813.916 kb on - strand at 3813.976 kb on + strand at 3813.976 kb on + strand at 3813.976 kb on + strand at 3813.977 kb on - strand at 3814.120 kb on - strand, within RR42_RS17625 at 3814.120 kb on - strand, within RR42_RS17625 at 3814.120 kb on - strand, within RR42_RS17625 at 3814.120 kb on - strand, within RR42_RS17625 at 3814.120 kb on - strand, within RR42_RS17625 at 3814.120 kb on - strand, within RR42_RS17625 at 3814.160 kb on + strand, within RR42_RS17625 at 3814.160 kb on + strand, within RR42_RS17625 at 3814.160 kb on + strand, within RR42_RS17625 at 3814.161 kb on - strand, within RR42_RS17625 at 3814.445 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,813,411 - -2.4 3,813,411 - -2.8 3,813,567 + RR42_RS17620 0.32 -1.5 3,813,567 + RR42_RS17620 0.32 -2.1 3,813,568 - RR42_RS17620 0.32 +0.2 3,813,582 + RR42_RS17620 0.34 -0.8 3,813,582 + RR42_RS17620 0.34 -0.8 3,813,583 - RR42_RS17620 0.35 -2.3 3,813,635 - RR42_RS17620 0.44 +0.0 3,813,849 - RR42_RS17620 0.81 -2.6 3,813,915 + -1.0 3,813,916 - -2.1 3,813,976 + +0.2 3,813,976 + +1.4 3,813,976 + +0.5 3,813,977 - +0.7 3,814,120 - RR42_RS17625 0.26 -0.8 3,814,120 - RR42_RS17625 0.26 +0.1 3,814,120 - RR42_RS17625 0.26 +0.3 3,814,120 - RR42_RS17625 0.26 -0.5 3,814,120 - RR42_RS17625 0.26 +0.8 3,814,120 - RR42_RS17625 0.26 +0.3 3,814,160 + RR42_RS17625 0.34 -0.4 3,814,160 + RR42_RS17625 0.34 -1.2 3,814,160 + RR42_RS17625 0.34 -0.6 3,814,161 - RR42_RS17625 0.34 -0.0 3,814,445 + -0.5
Or see this region's nucleotide sequence