Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16930 and RR42_RS16935 overlap by 4 nucleotides RR42_RS16935 and RR42_RS16940 are separated by 13 nucleotides RR42_RS16940 and RR42_RS16945 are separated by 466 nucleotides
RR42_RS16930: RR42_RS16930 - sulfonate ABC transporter ATP-binding protein, at 3,664,590 to 3,665,369
_RS16930
RR42_RS16935: RR42_RS16935 - ABC transporter permease, at 3,665,366 to 3,666,238
_RS16935
RR42_RS16940: RR42_RS16940 - ABC transporter permease, at 3,666,252 to 3,667,223
_RS16940
RR42_RS16945: RR42_RS16945 - NAD-dependent dehydratase, at 3,667,690 to 3,668,670
_RS16945
Position (kb)
3666
3667
3668 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3665.506 kb on + strand, within RR42_RS16935 at 3665.506 kb on + strand, within RR42_RS16935 at 3665.506 kb on + strand, within RR42_RS16935 at 3665.506 kb on + strand, within RR42_RS16935 at 3665.507 kb on - strand, within RR42_RS16935 at 3665.507 kb on - strand, within RR42_RS16935 at 3665.553 kb on + strand, within RR42_RS16935 at 3665.553 kb on + strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.554 kb on - strand, within RR42_RS16935 at 3665.661 kb on + strand, within RR42_RS16935 at 3665.662 kb on - strand, within RR42_RS16935 at 3665.820 kb on + strand, within RR42_RS16935 at 3665.869 kb on - strand, within RR42_RS16935 at 3665.939 kb on + strand, within RR42_RS16935 at 3665.940 kb on - strand, within RR42_RS16935 at 3665.971 kb on - strand, within RR42_RS16935 at 3665.981 kb on + strand, within RR42_RS16935 at 3665.981 kb on + strand, within RR42_RS16935 at 3665.981 kb on + strand, within RR42_RS16935 at 3665.981 kb on + strand, within RR42_RS16935 at 3665.982 kb on - strand, within RR42_RS16935 at 3665.982 kb on - strand, within RR42_RS16935 at 3665.982 kb on - strand, within RR42_RS16935 at 3666.253 kb on + strand at 3666.253 kb on + strand at 3666.254 kb on - strand at 3666.275 kb on - strand at 3666.375 kb on - strand, within RR42_RS16940 at 3666.511 kb on + strand, within RR42_RS16940 at 3666.511 kb on + strand, within RR42_RS16940 at 3666.512 kb on - strand, within RR42_RS16940 at 3666.596 kb on - strand, within RR42_RS16940 at 3666.655 kb on + strand, within RR42_RS16940 at 3666.656 kb on - strand, within RR42_RS16940 at 3666.665 kb on - strand, within RR42_RS16940 at 3666.665 kb on - strand, within RR42_RS16940 at 3666.665 kb on - strand, within RR42_RS16940 at 3666.665 kb on - strand, within RR42_RS16940 at 3666.790 kb on + strand, within RR42_RS16940 at 3666.894 kb on - strand, within RR42_RS16940 at 3667.179 kb on + strand at 3667.192 kb on - strand at 3667.199 kb on + strand at 3667.200 kb on - strand at 3667.306 kb on - strand at 3667.337 kb on + strand at 3667.337 kb on + strand at 3667.338 kb on - strand at 3667.338 kb on - strand at 3667.338 kb on - strand at 3667.349 kb on - strand at 3667.349 kb on - strand at 3667.352 kb on + strand at 3667.410 kb on + strand at 3667.411 kb on - strand at 3667.411 kb on - strand at 3667.411 kb on - strand at 3667.461 kb on + strand at 3667.578 kb on - strand at 3667.688 kb on - strand at 3667.884 kb on - strand, within RR42_RS16945 at 3667.998 kb on + strand, within RR42_RS16945 at 3667.998 kb on + strand, within RR42_RS16945 at 3667.998 kb on + strand, within RR42_RS16945 at 3667.999 kb on - strand, within RR42_RS16945 at 3668.000 kb on + strand, within RR42_RS16945 at 3668.000 kb on + strand, within RR42_RS16945 at 3668.000 kb on + strand, within RR42_RS16945 at 3668.000 kb on + strand, within RR42_RS16945 at 3668.001 kb on - strand, within RR42_RS16945 at 3668.001 kb on - strand, within RR42_RS16945 at 3668.001 kb on - strand, within RR42_RS16945 at 3668.001 kb on - strand, within RR42_RS16945 at 3668.001 kb on - strand, within RR42_RS16945 at 3668.072 kb on + strand, within RR42_RS16945 at 3668.072 kb on + strand, within RR42_RS16945 at 3668.073 kb on - strand, within RR42_RS16945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,665,506 + RR42_RS16935 0.16 -0.3 3,665,506 + RR42_RS16935 0.16 -0.4 3,665,506 + RR42_RS16935 0.16 -0.8 3,665,506 + RR42_RS16935 0.16 +0.6 3,665,507 - RR42_RS16935 0.16 +0.7 3,665,507 - RR42_RS16935 0.16 +0.7 3,665,553 + RR42_RS16935 0.21 -0.4 3,665,553 + RR42_RS16935 0.21 -1.1 3,665,554 - RR42_RS16935 0.22 -1.5 3,665,554 - RR42_RS16935 0.22 +0.0 3,665,554 - RR42_RS16935 0.22 -0.1 3,665,554 - RR42_RS16935 0.22 +1.3 3,665,554 - RR42_RS16935 0.22 -0.2 3,665,554 - RR42_RS16935 0.22 -0.6 3,665,661 + RR42_RS16935 0.34 -0.5 3,665,662 - RR42_RS16935 0.34 +0.8 3,665,820 + RR42_RS16935 0.52 -0.1 3,665,869 - RR42_RS16935 0.58 +1.0 3,665,939 + RR42_RS16935 0.66 +0.4 3,665,940 - RR42_RS16935 0.66 +0.2 3,665,971 - RR42_RS16935 0.69 +0.3 3,665,981 + RR42_RS16935 0.70 -0.5 3,665,981 + RR42_RS16935 0.70 -0.9 3,665,981 + RR42_RS16935 0.70 -1.9 3,665,981 + RR42_RS16935 0.70 +0.5 3,665,982 - RR42_RS16935 0.71 -0.9 3,665,982 - RR42_RS16935 0.71 -0.3 3,665,982 - RR42_RS16935 0.71 -0.0 3,666,253 + -0.7 3,666,253 + -1.1 3,666,254 - -0.3 3,666,275 - +0.8 3,666,375 - RR42_RS16940 0.13 -0.1 3,666,511 + RR42_RS16940 0.27 +0.1 3,666,511 + RR42_RS16940 0.27 +1.5 3,666,512 - RR42_RS16940 0.27 -1.2 3,666,596 - RR42_RS16940 0.35 +1.2 3,666,655 + RR42_RS16940 0.41 +1.2 3,666,656 - RR42_RS16940 0.42 -0.6 3,666,665 - RR42_RS16940 0.42 +0.2 3,666,665 - RR42_RS16940 0.42 +1.4 3,666,665 - RR42_RS16940 0.42 +0.3 3,666,665 - RR42_RS16940 0.42 -0.1 3,666,790 + RR42_RS16940 0.55 +0.4 3,666,894 - RR42_RS16940 0.66 +1.2 3,667,179 + +1.2 3,667,192 - -0.5 3,667,199 + -0.5 3,667,200 - +0.2 3,667,306 - -0.2 3,667,337 + -1.2 3,667,337 + +0.3 3,667,338 - -0.4 3,667,338 - -1.6 3,667,338 - -0.4 3,667,349 - -0.4 3,667,349 - +2.1 3,667,352 + -0.7 3,667,410 + +0.5 3,667,411 - -0.8 3,667,411 - +0.3 3,667,411 - -0.6 3,667,461 + +0.1 3,667,578 - +1.2 3,667,688 - +1.2 3,667,884 - RR42_RS16945 0.20 -0.3 3,667,998 + RR42_RS16945 0.31 +1.7 3,667,998 + RR42_RS16945 0.31 -0.5 3,667,998 + RR42_RS16945 0.31 +0.1 3,667,999 - RR42_RS16945 0.31 -1.0 3,668,000 + RR42_RS16945 0.32 +0.2 3,668,000 + RR42_RS16945 0.32 +0.7 3,668,000 + RR42_RS16945 0.32 -1.1 3,668,000 + RR42_RS16945 0.32 +0.6 3,668,001 - RR42_RS16945 0.32 +1.2 3,668,001 - RR42_RS16945 0.32 +0.1 3,668,001 - RR42_RS16945 0.32 -0.7 3,668,001 - RR42_RS16945 0.32 +1.0 3,668,001 - RR42_RS16945 0.32 +0.6 3,668,072 + RR42_RS16945 0.39 +1.2 3,668,072 + RR42_RS16945 0.39 -0.1 3,668,073 - RR42_RS16945 0.39 +0.2
Or see this region's nucleotide sequence