Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16055 and RR42_RS16060 are separated by 502 nucleotides RR42_RS16060 and RR42_RS16065 are separated by 224 nucleotides RR42_RS16065 and RR42_RS16070 overlap by 50 nucleotides
RR42_RS16055: RR42_RS16055 - pantothenate synthetase, at 3,477,793 to 3,478,641
_RS16055
RR42_RS16060: RR42_RS16060 - membrane protein, at 3,479,144 to 3,480,319
_RS16060
RR42_RS16065: RR42_RS16065 - GNAT family acetyltransferase, at 3,480,544 to 3,481,125
_RS16065
RR42_RS16070: RR42_RS16070 - cobyrinic acid a,c-diamide synthase, at 3,481,076 to 3,481,726
_RS16070
Position (kb)
3479
3480
3481 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3478.683 kb on + strand at 3478.683 kb on + strand at 3478.683 kb on + strand at 3478.683 kb on + strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.851 kb on - strand at 3478.967 kb on + strand at 3479.033 kb on + strand at 3479.033 kb on + strand at 3479.257 kb on + strand at 3479.294 kb on - strand, within RR42_RS16060 at 3479.294 kb on - strand, within RR42_RS16060 at 3479.305 kb on - strand, within RR42_RS16060 at 3479.305 kb on - strand, within RR42_RS16060 at 3479.375 kb on - strand, within RR42_RS16060 at 3479.502 kb on + strand, within RR42_RS16060 at 3479.503 kb on - strand, within RR42_RS16060 at 3479.540 kb on + strand, within RR42_RS16060 at 3479.540 kb on + strand, within RR42_RS16060 at 3479.540 kb on + strand, within RR42_RS16060 at 3479.540 kb on + strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.719 kb on + strand, within RR42_RS16060 at 3479.719 kb on + strand, within RR42_RS16060 at 3479.720 kb on - strand, within RR42_RS16060 at 3479.880 kb on + strand, within RR42_RS16060 at 3480.028 kb on - strand, within RR42_RS16060 at 3480.038 kb on - strand, within RR42_RS16060 at 3480.087 kb on + strand, within RR42_RS16060 at 3480.087 kb on + strand, within RR42_RS16060 at 3480.152 kb on + strand, within RR42_RS16060 at 3480.153 kb on - strand, within RR42_RS16060 at 3480.153 kb on - strand, within RR42_RS16060 at 3480.274 kb on - strand at 3480.431 kb on + strand at 3480.431 kb on + strand at 3480.432 kb on - strand at 3480.631 kb on + strand, within RR42_RS16065 at 3480.631 kb on + strand, within RR42_RS16065 at 3480.631 kb on + strand, within RR42_RS16065 at 3480.632 kb on - strand, within RR42_RS16065 at 3480.632 kb on - strand, within RR42_RS16065 at 3480.805 kb on + strand, within RR42_RS16065 at 3480.806 kb on - strand, within RR42_RS16065 at 3480.806 kb on - strand, within RR42_RS16065 at 3480.814 kb on + strand, within RR42_RS16065 at 3480.814 kb on + strand, within RR42_RS16065 at 3480.815 kb on - strand, within RR42_RS16065 at 3480.815 kb on - strand, within RR42_RS16065 at 3480.931 kb on + strand, within RR42_RS16065 at 3480.931 kb on + strand, within RR42_RS16065 at 3480.932 kb on - strand, within RR42_RS16065 at 3480.932 kb on - strand, within RR42_RS16065 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.210 kb on - strand, within RR42_RS16070 at 3481.210 kb on - strand, within RR42_RS16070
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,478,683 + +0.8 3,478,683 + +0.2 3,478,683 + -0.0 3,478,683 + +0.5 3,478,684 - -0.9 3,478,684 - +0.1 3,478,684 - +0.1 3,478,684 - +0.6 3,478,684 - +1.0 3,478,684 - +0.9 3,478,684 - +0.0 3,478,684 - -0.8 3,478,851 - -1.2 3,478,967 + +0.2 3,479,033 + -0.0 3,479,033 + -0.3 3,479,257 + +0.6 3,479,294 - RR42_RS16060 0.13 -0.7 3,479,294 - RR42_RS16060 0.13 -0.6 3,479,305 - RR42_RS16060 0.14 +0.8 3,479,305 - RR42_RS16060 0.14 +0.8 3,479,375 - RR42_RS16060 0.20 +0.7 3,479,502 + RR42_RS16060 0.30 -0.5 3,479,503 - RR42_RS16060 0.31 +0.6 3,479,540 + RR42_RS16060 0.34 +0.4 3,479,540 + RR42_RS16060 0.34 +1.2 3,479,540 + RR42_RS16060 0.34 +1.8 3,479,540 + RR42_RS16060 0.34 -0.6 3,479,541 - RR42_RS16060 0.34 +0.1 3,479,541 - RR42_RS16060 0.34 -0.6 3,479,541 - RR42_RS16060 0.34 +1.2 3,479,541 - RR42_RS16060 0.34 +0.8 3,479,541 - RR42_RS16060 0.34 -0.0 3,479,719 + RR42_RS16060 0.49 -0.1 3,479,719 + RR42_RS16060 0.49 +0.3 3,479,720 - RR42_RS16060 0.49 +0.3 3,479,880 + RR42_RS16060 0.63 -0.9 3,480,028 - RR42_RS16060 0.75 -2.0 3,480,038 - RR42_RS16060 0.76 -0.8 3,480,087 + RR42_RS16060 0.80 +1.2 3,480,087 + RR42_RS16060 0.80 +0.6 3,480,152 + RR42_RS16060 0.86 +0.8 3,480,153 - RR42_RS16060 0.86 -1.3 3,480,153 - RR42_RS16060 0.86 -0.4 3,480,274 - +0.3 3,480,431 + -1.0 3,480,431 + -0.6 3,480,432 - -0.1 3,480,631 + RR42_RS16065 0.15 +1.8 3,480,631 + RR42_RS16065 0.15 +0.7 3,480,631 + RR42_RS16065 0.15 +1.5 3,480,632 - RR42_RS16065 0.15 +0.1 3,480,632 - RR42_RS16065 0.15 +0.7 3,480,805 + RR42_RS16065 0.45 -0.1 3,480,806 - RR42_RS16065 0.45 +0.5 3,480,806 - RR42_RS16065 0.45 +0.5 3,480,814 + RR42_RS16065 0.46 +0.2 3,480,814 + RR42_RS16065 0.46 +1.2 3,480,815 - RR42_RS16065 0.47 +0.5 3,480,815 - RR42_RS16065 0.47 +0.8 3,480,931 + RR42_RS16065 0.66 +0.9 3,480,931 + RR42_RS16065 0.66 +1.0 3,480,932 - RR42_RS16065 0.67 +0.1 3,480,932 - RR42_RS16065 0.67 -0.4 3,481,209 + RR42_RS16070 0.20 +0.0 3,481,209 + RR42_RS16070 0.20 +0.0 3,481,209 + RR42_RS16070 0.20 -0.9 3,481,209 + RR42_RS16070 0.20 +0.2 3,481,209 + RR42_RS16070 0.20 +0.9 3,481,210 - RR42_RS16070 0.21 +0.2 3,481,210 - RR42_RS16070 0.21 +0.0
Or see this region's nucleotide sequence