Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15725 and RR42_RS15730 are separated by 48 nucleotides RR42_RS15730 and RR42_RS15735 overlap by 1 nucleotides RR42_RS15735 and RR42_RS15740 are separated by 214 nucleotides RR42_RS15740 and RR42_RS15745 are separated by 60 nucleotides
RR42_RS15725: RR42_RS15725 - hypothetical protein, at 3,399,837 to 3,400,478
_RS15725
RR42_RS15730: RR42_RS15730 - sugar transferase, at 3,400,527 to 3,401,123
_RS15730
RR42_RS15735: RR42_RS15735 - pyridoxal phosphate-dependent aminotransferase, at 3,401,123 to 3,402,277
_RS15735
RR42_RS15740: RR42_RS15740 - hypothetical protein, at 3,402,492 to 3,402,968
_RS15740
RR42_RS15745: RR42_RS15745 - group1 glycosyl transferase, at 3,403,029 to 3,404,264
_RS15745
Position (kb)
3401
3402
3403 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3400.225 kb on + strand, within RR42_RS15725 at 3400.226 kb on - strand, within RR42_RS15725 at 3400.559 kb on + strand at 3400.598 kb on - strand, within RR42_RS15730 at 3400.598 kb on - strand, within RR42_RS15730 at 3400.598 kb on - strand, within RR42_RS15730 at 3400.610 kb on - strand, within RR42_RS15730 at 3400.641 kb on - strand, within RR42_RS15730 at 3400.642 kb on + strand, within RR42_RS15730 at 3400.643 kb on - strand, within RR42_RS15730 at 3400.643 kb on - strand, within RR42_RS15730 at 3400.762 kb on + strand, within RR42_RS15730 at 3400.774 kb on + strand, within RR42_RS15730 at 3400.774 kb on + strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.775 kb on - strand, within RR42_RS15730 at 3400.858 kb on + strand, within RR42_RS15730 at 3400.858 kb on + strand, within RR42_RS15730 at 3400.890 kb on - strand, within RR42_RS15730 at 3400.958 kb on + strand, within RR42_RS15730 at 3400.958 kb on + strand, within RR42_RS15730 at 3400.959 kb on - strand, within RR42_RS15730 at 3401.059 kb on - strand, within RR42_RS15730 at 3401.129 kb on - strand at 3401.179 kb on + strand at 3401.187 kb on + strand at 3401.200 kb on + strand at 3401.200 kb on + strand at 3401.214 kb on + strand at 3401.271 kb on + strand, within RR42_RS15735 at 3401.296 kb on - strand, within RR42_RS15735 at 3401.296 kb on - strand, within RR42_RS15735 at 3401.306 kb on - strand, within RR42_RS15735 at 3401.385 kb on + strand, within RR42_RS15735 at 3401.385 kb on + strand, within RR42_RS15735 at 3401.405 kb on - strand, within RR42_RS15735 at 3401.556 kb on + strand, within RR42_RS15735 at 3401.557 kb on - strand, within RR42_RS15735 at 3401.557 kb on - strand, within RR42_RS15735 at 3401.584 kb on - strand, within RR42_RS15735 at 3401.586 kb on + strand, within RR42_RS15735 at 3401.587 kb on - strand, within RR42_RS15735 at 3401.587 kb on - strand, within RR42_RS15735 at 3401.785 kb on + strand, within RR42_RS15735 at 3401.786 kb on - strand, within RR42_RS15735 at 3401.979 kb on - strand, within RR42_RS15735 at 3402.040 kb on + strand, within RR42_RS15735 at 3402.040 kb on + strand, within RR42_RS15735 at 3402.041 kb on - strand, within RR42_RS15735 at 3402.126 kb on - strand, within RR42_RS15735 at 3402.126 kb on - strand, within RR42_RS15735 at 3402.269 kb on - strand at 3402.269 kb on - strand at 3402.280 kb on + strand at 3402.281 kb on - strand at 3402.281 kb on - strand at 3402.299 kb on - strand at 3402.312 kb on - strand at 3402.478 kb on + strand at 3402.586 kb on - strand, within RR42_RS15740 at 3402.630 kb on - strand, within RR42_RS15740 at 3402.714 kb on + strand, within RR42_RS15740 at 3402.929 kb on - strand at 3403.004 kb on - strand at 3403.004 kb on - strand at 3403.011 kb on - strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.019 kb on + strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.020 kb on - strand at 3403.121 kb on + strand at 3403.192 kb on + strand, within RR42_RS15745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,400,225 + RR42_RS15725 0.60 -0.8 3,400,226 - RR42_RS15725 0.61 -0.1 3,400,559 + -0.7 3,400,598 - RR42_RS15730 0.12 -0.2 3,400,598 - RR42_RS15730 0.12 -0.7 3,400,598 - RR42_RS15730 0.12 -3.0 3,400,610 - RR42_RS15730 0.14 -0.0 3,400,641 - RR42_RS15730 0.19 -1.1 3,400,642 + RR42_RS15730 0.19 -1.2 3,400,643 - RR42_RS15730 0.19 -0.1 3,400,643 - RR42_RS15730 0.19 -1.5 3,400,762 + RR42_RS15730 0.39 -1.6 3,400,774 + RR42_RS15730 0.41 -1.9 3,400,774 + RR42_RS15730 0.41 -1.6 3,400,775 - RR42_RS15730 0.42 -1.2 3,400,775 - RR42_RS15730 0.42 -1.5 3,400,775 - RR42_RS15730 0.42 +0.4 3,400,775 - RR42_RS15730 0.42 -1.9 3,400,858 + RR42_RS15730 0.55 -1.8 3,400,858 + RR42_RS15730 0.55 -0.3 3,400,890 - RR42_RS15730 0.61 -1.6 3,400,958 + RR42_RS15730 0.72 -0.7 3,400,958 + RR42_RS15730 0.72 -1.2 3,400,959 - RR42_RS15730 0.72 -0.2 3,401,059 - RR42_RS15730 0.89 -1.5 3,401,129 - -1.7 3,401,179 + -2.1 3,401,187 + -1.1 3,401,200 + -1.5 3,401,200 + +0.8 3,401,214 + -0.9 3,401,271 + RR42_RS15735 0.13 -0.7 3,401,296 - RR42_RS15735 0.15 -1.5 3,401,296 - RR42_RS15735 0.15 -1.5 3,401,306 - RR42_RS15735 0.16 -0.9 3,401,385 + RR42_RS15735 0.23 -1.5 3,401,385 + RR42_RS15735 0.23 -1.1 3,401,405 - RR42_RS15735 0.24 +1.3 3,401,556 + RR42_RS15735 0.37 -2.1 3,401,557 - RR42_RS15735 0.38 -1.7 3,401,557 - RR42_RS15735 0.38 -1.2 3,401,584 - RR42_RS15735 0.40 -0.4 3,401,586 + RR42_RS15735 0.40 -0.2 3,401,587 - RR42_RS15735 0.40 -0.5 3,401,587 - RR42_RS15735 0.40 -1.6 3,401,785 + RR42_RS15735 0.57 -1.4 3,401,786 - RR42_RS15735 0.57 +0.0 3,401,979 - RR42_RS15735 0.74 -2.5 3,402,040 + RR42_RS15735 0.79 -0.2 3,402,040 + RR42_RS15735 0.79 -1.3 3,402,041 - RR42_RS15735 0.79 -1.0 3,402,126 - RR42_RS15735 0.87 -0.7 3,402,126 - RR42_RS15735 0.87 -0.6 3,402,269 - -0.8 3,402,269 - -1.1 3,402,280 + -0.7 3,402,281 - +0.0 3,402,281 - +0.0 3,402,299 - +0.0 3,402,312 - -2.8 3,402,478 + -1.3 3,402,586 - RR42_RS15740 0.20 -0.1 3,402,630 - RR42_RS15740 0.29 -3.9 3,402,714 + RR42_RS15740 0.47 -2.8 3,402,929 - -0.1 3,403,004 - -1.2 3,403,004 - -1.9 3,403,011 - -2.2 3,403,019 + -1.2 3,403,019 + -1.2 3,403,019 + -0.3 3,403,019 + -3.3 3,403,020 - +0.6 3,403,020 - +0.8 3,403,020 - +0.4 3,403,020 - +3.3 3,403,121 + -2.3 3,403,192 + RR42_RS15745 0.13 -1.8
Or see this region's nucleotide sequence