Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15515 and RR42_RS15520 are separated by 38 nucleotides RR42_RS15520 and RR42_RS15525 are separated by 186 nucleotides RR42_RS15525 and RR42_RS15530 overlap by 4 nucleotides
RR42_RS15515: RR42_RS15515 - hypothetical protein, at 3,349,352 to 3,354,169
_RS15515
RR42_RS15520: RR42_RS15520 - hypothetical protein, at 3,354,208 to 3,354,639
_RS15520
RR42_RS15525: RR42_RS15525 - hypothetical protein, at 3,354,826 to 3,355,266
_RS15525
RR42_RS15530: RR42_RS15530 - membrane protein, at 3,355,263 to 3,356,195
_RS15530
Position (kb)
3354
3355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3353.237 kb on + strand, within RR42_RS15515 at 3353.237 kb on + strand, within RR42_RS15515 at 3353.238 kb on - strand, within RR42_RS15515 at 3353.510 kb on + strand, within RR42_RS15515 at 3353.564 kb on - strand, within RR42_RS15515 at 3353.672 kb on + strand, within RR42_RS15515 at 3353.673 kb on - strand, within RR42_RS15515 at 3353.684 kb on + strand, within RR42_RS15515 at 3353.685 kb on - strand, within RR42_RS15515 at 3353.685 kb on - strand, within RR42_RS15515 at 3353.714 kb on + strand at 3353.715 kb on - strand at 3353.741 kb on + strand at 3353.742 kb on - strand at 3353.792 kb on + strand at 3353.792 kb on + strand at 3353.818 kb on + strand at 3353.818 kb on + strand at 3353.819 kb on - strand at 3353.819 kb on - strand at 3353.934 kb on + strand at 3353.935 kb on - strand at 3354.028 kb on + strand at 3354.028 kb on + strand at 3354.029 kb on - strand at 3354.180 kb on + strand at 3354.180 kb on + strand at 3354.181 kb on - strand at 3354.181 kb on - strand at 3354.201 kb on + strand at 3354.218 kb on + strand at 3354.219 kb on - strand at 3354.219 kb on - strand at 3354.219 kb on - strand at 3354.249 kb on + strand at 3354.325 kb on + strand, within RR42_RS15520 at 3354.325 kb on + strand, within RR42_RS15520 at 3354.326 kb on - strand, within RR42_RS15520 at 3354.326 kb on - strand, within RR42_RS15520 at 3354.397 kb on + strand, within RR42_RS15520 at 3354.415 kb on + strand, within RR42_RS15520 at 3354.415 kb on + strand, within RR42_RS15520 at 3354.481 kb on + strand, within RR42_RS15520 at 3354.482 kb on - strand, within RR42_RS15520 at 3354.526 kb on + strand, within RR42_RS15520 at 3354.526 kb on + strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.715 kb on + strand at 3354.716 kb on - strand at 3354.756 kb on - strand at 3354.790 kb on + strand at 3354.998 kb on - strand, within RR42_RS15525 at 3355.378 kb on - strand, within RR42_RS15530 at 3355.458 kb on - strand, within RR42_RS15530 at 3355.556 kb on + strand, within RR42_RS15530 at 3355.557 kb on - strand, within RR42_RS15530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,353,237 + RR42_RS15515 0.81 +0.9 3,353,237 + RR42_RS15515 0.81 -2.8 3,353,238 - RR42_RS15515 0.81 -0.1 3,353,510 + RR42_RS15515 0.86 -0.2 3,353,564 - RR42_RS15515 0.87 -0.3 3,353,672 + RR42_RS15515 0.90 -1.2 3,353,673 - RR42_RS15515 0.90 -0.7 3,353,684 + RR42_RS15515 0.90 +1.3 3,353,685 - RR42_RS15515 0.90 +1.0 3,353,685 - RR42_RS15515 0.90 +0.3 3,353,714 + +0.8 3,353,715 - -1.6 3,353,741 + -0.4 3,353,742 - +0.2 3,353,792 + +1.0 3,353,792 + +0.1 3,353,818 + +0.9 3,353,818 + +1.1 3,353,819 - +1.1 3,353,819 - -0.3 3,353,934 + +0.5 3,353,935 - -0.1 3,354,028 + +1.3 3,354,028 + -0.1 3,354,029 - +0.6 3,354,180 + +1.4 3,354,180 + +1.3 3,354,181 - +0.6 3,354,181 - +2.5 3,354,201 + -1.1 3,354,218 + +0.4 3,354,219 - +1.9 3,354,219 - +0.0 3,354,219 - +1.3 3,354,249 + +1.2 3,354,325 + RR42_RS15520 0.27 +0.3 3,354,325 + RR42_RS15520 0.27 +0.3 3,354,326 - RR42_RS15520 0.27 -0.4 3,354,326 - RR42_RS15520 0.27 +1.3 3,354,397 + RR42_RS15520 0.44 -1.2 3,354,415 + RR42_RS15520 0.48 -0.1 3,354,415 + RR42_RS15520 0.48 -0.2 3,354,481 + RR42_RS15520 0.63 -0.7 3,354,482 - RR42_RS15520 0.63 -0.1 3,354,526 + RR42_RS15520 0.74 -0.7 3,354,526 + RR42_RS15520 0.74 -0.4 3,354,527 - RR42_RS15520 0.74 +0.8 3,354,527 - RR42_RS15520 0.74 +0.2 3,354,527 - RR42_RS15520 0.74 +0.4 3,354,715 + -0.5 3,354,716 - -0.1 3,354,756 - +0.5 3,354,790 + +0.8 3,354,998 - RR42_RS15525 0.39 -2.2 3,355,378 - RR42_RS15530 0.12 +0.1 3,355,458 - RR42_RS15530 0.21 -1.1 3,355,556 + RR42_RS15530 0.31 +0.4 3,355,557 - RR42_RS15530 0.32 -0.8
Or see this region's nucleotide sequence