Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15485 and RR42_RS15490 are separated by 146 nucleotides RR42_RS15490 and RR42_RS15495 are separated by 325 nucleotides RR42_RS15495 and RR42_RS15500 are separated by 232 nucleotides
RR42_RS15485: RR42_RS15485 - serine hydroxymethyltransferase, at 3,344,702 to 3,345,949
_RS15485
RR42_RS15490: RR42_RS15490 - NrdR family transcriptional regulator, at 3,346,096 to 3,346,563
_RS15490
RR42_RS15495: RR42_RS15495 - hypothetical protein, at 3,346,889 to 3,347,170
_RS15495
RR42_RS15500: RR42_RS15500 - hypothetical protein, at 3,347,403 to 3,347,846
_RS15500
Position (kb)
3346
3347 Strain fitness (log2 ratio)
-2
-1
0
1 at 3346.077 kb on + strand at 3346.276 kb on + strand, within RR42_RS15490 at 3346.278 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.466 kb on - strand, within RR42_RS15490 at 3346.466 kb on - strand, within RR42_RS15490 at 3346.487 kb on - strand, within RR42_RS15490 at 3346.604 kb on - strand at 3346.604 kb on - strand at 3346.650 kb on - strand at 3346.799 kb on + strand at 3346.799 kb on + strand at 3346.799 kb on + strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.857 kb on + strand at 3347.010 kb on + strand, within RR42_RS15495 at 3347.010 kb on + strand, within RR42_RS15495 at 3347.044 kb on + strand, within RR42_RS15495 at 3347.044 kb on + strand, within RR42_RS15495 at 3347.045 kb on - strand, within RR42_RS15495 at 3347.045 kb on - strand, within RR42_RS15495 at 3347.045 kb on - strand, within RR42_RS15495 at 3347.146 kb on + strand at 3347.146 kb on + strand at 3347.147 kb on - strand at 3347.147 kb on - strand at 3347.163 kb on + strand at 3347.164 kb on - strand at 3347.164 kb on - strand at 3347.212 kb on + strand at 3347.330 kb on - strand at 3347.518 kb on + strand, within RR42_RS15500 at 3347.519 kb on - strand, within RR42_RS15500 at 3347.519 kb on - strand, within RR42_RS15500 at 3347.519 kb on - strand, within RR42_RS15500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,346,077 + -1.1 3,346,276 + RR42_RS15490 0.38 -0.7 3,346,278 + RR42_RS15490 0.39 -1.4 3,346,465 + RR42_RS15490 0.79 -0.4 3,346,465 + RR42_RS15490 0.79 -1.4 3,346,465 + RR42_RS15490 0.79 -1.3 3,346,465 + RR42_RS15490 0.79 -0.1 3,346,465 + RR42_RS15490 0.79 -0.1 3,346,466 - RR42_RS15490 0.79 -2.3 3,346,466 - RR42_RS15490 0.79 +0.0 3,346,487 - RR42_RS15490 0.84 -2.3 3,346,604 - -1.4 3,346,604 - +1.6 3,346,650 - +0.2 3,346,799 + +0.9 3,346,799 + -0.3 3,346,799 + -0.2 3,346,800 - +0.1 3,346,800 - -0.5 3,346,800 - +1.1 3,346,800 - +0.1 3,346,800 - -0.3 3,346,800 - -0.2 3,346,800 - +0.7 3,346,800 - +1.2 3,346,800 - +0.2 3,346,800 - +0.8 3,346,857 + +0.2 3,347,010 + RR42_RS15495 0.43 -1.2 3,347,010 + RR42_RS15495 0.43 -0.2 3,347,044 + RR42_RS15495 0.55 +0.3 3,347,044 + RR42_RS15495 0.55 +1.0 3,347,045 - RR42_RS15495 0.55 -0.2 3,347,045 - RR42_RS15495 0.55 +0.1 3,347,045 - RR42_RS15495 0.55 -1.1 3,347,146 + +0.2 3,347,146 + -0.1 3,347,147 - -0.2 3,347,147 - -0.3 3,347,163 + -0.5 3,347,164 - +0.1 3,347,164 - -0.0 3,347,212 + -0.2 3,347,330 - -1.8 3,347,518 + RR42_RS15500 0.26 +1.1 3,347,519 - RR42_RS15500 0.26 -0.9 3,347,519 - RR42_RS15500 0.26 -0.1 3,347,519 - RR42_RS15500 0.26 +0.6
Or see this region's nucleotide sequence