Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15465 and RR42_RS15470 are separated by 15 nucleotides RR42_RS15470 and RR42_RS15475 are separated by 42 nucleotides RR42_RS15475 and RR42_RS15480 overlap by 4 nucleotides RR42_RS15480 and RR42_RS15485 are separated by 385 nucleotides
RR42_RS15465: RR42_RS15465 - TonB-dependent receptor, at 3,341,582 to 3,342,736
_RS15465
RR42_RS15470: RR42_RS15470 - biopolymer transporter ExbD, at 3,342,752 to 3,343,189
_RS15470
RR42_RS15475: RR42_RS15475 - protein tolQ, at 3,343,232 to 3,343,915
_RS15475
RR42_RS15480: RR42_RS15480 - acyl-CoA thioesterase, at 3,343,912 to 3,344,316
_RS15480
RR42_RS15485: RR42_RS15485 - serine hydroxymethyltransferase, at 3,344,702 to 3,345,949
_RS15485
Position (kb)
3343
3344 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3342.512 kb on + strand, within RR42_RS15465 at 3342.634 kb on - strand at 3342.715 kb on - strand at 3343.122 kb on + strand, within RR42_RS15470 at 3343.122 kb on + strand, within RR42_RS15470 at 3343.464 kb on - strand, within RR42_RS15475 at 3343.486 kb on - strand, within RR42_RS15475 at 3343.486 kb on - strand, within RR42_RS15475 at 3343.684 kb on + strand, within RR42_RS15475 at 3343.786 kb on + strand, within RR42_RS15475 at 3343.795 kb on + strand, within RR42_RS15475 at 3343.795 kb on + strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.797 kb on + strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.974 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.124 kb on - strand, within RR42_RS15480 at 3344.124 kb on - strand, within RR42_RS15480 at 3344.241 kb on - strand, within RR42_RS15480 at 3344.257 kb on + strand, within RR42_RS15480 at 3344.258 kb on - strand, within RR42_RS15480 at 3344.286 kb on - strand at 3344.329 kb on - strand at 3344.371 kb on + strand at 3344.371 kb on + strand at 3344.371 kb on + strand at 3344.372 kb on - strand at 3344.380 kb on + strand at 3344.380 kb on + strand at 3344.381 kb on - strand at 3344.381 kb on - strand at 3344.512 kb on + strand at 3344.608 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,342,512 + RR42_RS15465 0.81 -2.7 3,342,634 - -2.6 3,342,715 - -1.3 3,343,122 + RR42_RS15470 0.84 -1.3 3,343,122 + RR42_RS15470 0.84 -3.7 3,343,464 - RR42_RS15475 0.34 -1.5 3,343,486 - RR42_RS15475 0.37 -2.3 3,343,486 - RR42_RS15475 0.37 -4.1 3,343,684 + RR42_RS15475 0.66 -1.9 3,343,786 + RR42_RS15475 0.81 -2.4 3,343,795 + RR42_RS15475 0.82 -0.7 3,343,795 + RR42_RS15475 0.82 -3.7 3,343,796 - RR42_RS15475 0.82 -2.5 3,343,796 - RR42_RS15475 0.82 -3.3 3,343,796 - RR42_RS15475 0.82 -0.6 3,343,796 - RR42_RS15475 0.82 -1.8 3,343,796 - RR42_RS15475 0.82 -3.4 3,343,796 - RR42_RS15475 0.82 -3.1 3,343,797 + RR42_RS15475 0.83 -2.1 3,343,798 - RR42_RS15475 0.83 +0.1 3,343,798 - RR42_RS15475 0.83 -1.3 3,343,798 - RR42_RS15475 0.83 -3.3 3,343,974 + RR42_RS15480 0.15 -4.3 3,344,123 + RR42_RS15480 0.52 -1.5 3,344,123 + RR42_RS15480 0.52 -0.9 3,344,123 + RR42_RS15480 0.52 -1.0 3,344,124 - RR42_RS15480 0.52 +0.3 3,344,124 - RR42_RS15480 0.52 +0.8 3,344,241 - RR42_RS15480 0.81 +0.1 3,344,257 + RR42_RS15480 0.85 -0.3 3,344,258 - RR42_RS15480 0.85 -2.8 3,344,286 - -2.0 3,344,329 - +2.3 3,344,371 + +0.3 3,344,371 + -0.1 3,344,371 + +0.8 3,344,372 - +1.7 3,344,380 + -0.0 3,344,380 + -0.6 3,344,381 - +0.8 3,344,381 - +0.3 3,344,512 + +0.5 3,344,608 + +0.3
Or see this region's nucleotide sequence