Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15000

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14995 and RR42_RS15000 are separated by 65 nucleotidesRR42_RS15000 and RR42_RS15005 are separated by 248 nucleotides RR42_RS14995: RR42_RS14995 - tRNA threonylcarbamoyladenosine modification protein TsaD, at 3,241,930 to 3,242,970 _RS14995 RR42_RS15000: RR42_RS15000 - GTP cyclohydrolase, at 3,243,036 to 3,243,839 _RS15000 RR42_RS15005: RR42_RS15005 - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,244,088 to 3,245,998 _RS15005 Position (kb) 3243 3244Strain fitness (log2 ratio) -1 0 1 2 3at 3242.990 kb on + strandat 3242.990 kb on + strandat 3243.037 kb on + strandat 3243.037 kb on + strandat 3243.071 kb on - strandat 3243.118 kb on + strand, within RR42_RS15000at 3243.119 kb on - strand, within RR42_RS15000at 3243.119 kb on - strand, within RR42_RS15000at 3243.119 kb on - strand, within RR42_RS15000at 3243.463 kb on + strand, within RR42_RS15000at 3243.463 kb on + strand, within RR42_RS15000at 3243.464 kb on - strand, within RR42_RS15000at 3243.858 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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3,242,990 + +0.2
3,242,990 + +0.9
3,243,037 + +0.7
3,243,037 + +1.3
3,243,071 - +1.1
3,243,118 + RR42_RS15000 0.10 +1.0
3,243,119 - RR42_RS15000 0.10 -0.3
3,243,119 - RR42_RS15000 0.10 +1.5
3,243,119 - RR42_RS15000 0.10 -0.7
3,243,463 + RR42_RS15000 0.53 +1.2
3,243,463 + RR42_RS15000 0.53 +0.4
3,243,464 - RR42_RS15000 0.53 +3.3
3,243,858 - -1.1

Or see this region's nucleotide sequence