Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14965 and RR42_RS14975 are separated by 486 nucleotides RR42_RS14975 and RR42_RS14980 are separated by 225 nucleotides RR42_RS14980 and RR42_RS14985 are separated by 130 nucleotides
RR42_RS14965: RR42_RS14965 - hypothetical protein, at 3,234,764 to 3,237,040
_RS14965
RR42_RS14975: RR42_RS14975 - DNA primase, at 3,237,527 to 3,239,341
_RS14975
RR42_RS14980: RR42_RS14980 - glutamyl-tRNA amidotransferase, at 3,239,567 to 3,240,016
_RS14980
RR42_RS14985: RR42_RS14985 - 30S ribosomal protein S21, at 3,240,147 to 3,240,359
_RS14985
Position (kb)
3237
3238
3239
3240 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3237.023 kb on - strand at 3237.026 kb on - strand at 3237.026 kb on - strand at 3237.026 kb on - strand at 3237.071 kb on - strand at 3237.071 kb on - strand at 3237.071 kb on - strand at 3237.071 kb on - strand at 3237.377 kb on + strand at 3237.377 kb on + strand at 3237.377 kb on + strand at 3237.378 kb on - strand at 3237.378 kb on - strand at 3237.383 kb on + strand at 3237.383 kb on + strand at 3237.384 kb on - strand at 3237.384 kb on - strand at 3237.437 kb on + strand at 3237.437 kb on + strand at 3237.469 kb on + strand at 3237.469 kb on + strand at 3237.470 kb on - strand at 3237.470 kb on - strand at 3237.470 kb on - strand at 3237.497 kb on + strand at 3239.357 kb on - strand at 3239.440 kb on - strand at 3239.446 kb on - strand at 3239.446 kb on - strand at 3239.453 kb on - strand at 3239.513 kb on + strand at 3239.551 kb on + strand at 3239.660 kb on - strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.751 kb on + strand, within RR42_RS14980 at 3239.752 kb on - strand, within RR42_RS14980 at 3240.022 kb on + strand at 3240.152 kb on - strand at 3240.152 kb on - strand at 3240.191 kb on - strand, within RR42_RS14985
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,237,023 - -0.9 3,237,026 - +0.2 3,237,026 - -0.7 3,237,026 - -1.1 3,237,071 - -0.1 3,237,071 - +0.5 3,237,071 - +0.2 3,237,071 - -1.1 3,237,377 + -1.4 3,237,377 + -1.7 3,237,377 + -0.3 3,237,378 - +0.3 3,237,378 - +0.8 3,237,383 + -0.7 3,237,383 + -2.0 3,237,384 - +1.7 3,237,384 - +1.0 3,237,437 + -0.3 3,237,437 + -2.8 3,237,469 + -0.9 3,237,469 + -2.6 3,237,470 - -1.0 3,237,470 - -1.2 3,237,470 - +1.6 3,237,497 + -1.2 3,239,357 - +0.4 3,239,440 - +0.7 3,239,446 - +0.8 3,239,446 - -1.9 3,239,453 - +0.0 3,239,513 + +0.3 3,239,551 + -0.6 3,239,660 - RR42_RS14980 0.21 +0.5 3,239,751 + RR42_RS14980 0.41 -2.4 3,239,751 + RR42_RS14980 0.41 -0.4 3,239,751 + RR42_RS14980 0.41 +0.3 3,239,752 - RR42_RS14980 0.41 +0.6 3,240,022 + -0.6 3,240,152 - +0.2 3,240,152 - -1.2 3,240,191 - RR42_RS14985 0.21 -2.3
Or see this region's nucleotide sequence