Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14925 and RR42_RS14930 are separated by 52 nucleotides RR42_RS14930 and RR42_RS14935 are separated by 15 nucleotides RR42_RS14935 and RR42_RS14940 are separated by 67 nucleotides
RR42_RS14925: RR42_RS14925 - hypothetical protein, at 3,227,966 to 3,228,634
_RS14925
RR42_RS14930: RR42_RS14930 - biopolymer transporter ExbD, at 3,228,687 to 3,229,097
_RS14930
RR42_RS14935: RR42_RS14935 - biopolymer transporter ExbB, at 3,229,113 to 3,230,912
_RS14935
RR42_RS14940: RR42_RS14940 - membrane protein, at 3,230,980 to 3,232,566
_RS14940
Position (kb)
3229
3230
3231 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3228.220 kb on - strand, within RR42_RS14925 at 3228.220 kb on - strand, within RR42_RS14925 at 3228.220 kb on - strand, within RR42_RS14925 at 3228.228 kb on + strand, within RR42_RS14925 at 3228.476 kb on - strand, within RR42_RS14925 at 3228.769 kb on + strand, within RR42_RS14930 at 3228.769 kb on + strand, within RR42_RS14930 at 3228.770 kb on - strand, within RR42_RS14930 at 3229.090 kb on + strand at 3229.090 kb on + strand at 3229.244 kb on - strand at 3229.579 kb on + strand, within RR42_RS14935 at 3229.579 kb on + strand, within RR42_RS14935 at 3230.254 kb on + strand, within RR42_RS14935 at 3230.254 kb on + strand, within RR42_RS14935 at 3230.494 kb on + strand, within RR42_RS14935 at 3230.551 kb on + strand, within RR42_RS14935 at 3230.551 kb on + strand, within RR42_RS14935 at 3230.552 kb on - strand, within RR42_RS14935 at 3230.554 kb on + strand, within RR42_RS14935 at 3230.825 kb on - strand at 3230.930 kb on - strand at 3230.930 kb on - strand at 3230.949 kb on + strand at 3230.949 kb on + strand at 3230.950 kb on - strand at 3230.985 kb on - strand at 3230.985 kb on - strand at 3231.112 kb on + strand at 3231.191 kb on + strand, within RR42_RS14940 at 3231.192 kb on - strand, within RR42_RS14940 at 3231.192 kb on - strand, within RR42_RS14940 at 3231.269 kb on + strand, within RR42_RS14940 at 3231.270 kb on - strand, within RR42_RS14940 at 3231.411 kb on - strand, within RR42_RS14940 at 3231.462 kb on - strand, within RR42_RS14940 at 3231.462 kb on - strand, within RR42_RS14940 at 3231.545 kb on + strand, within RR42_RS14940 at 3231.545 kb on + strand, within RR42_RS14940 at 3231.546 kb on - strand, within RR42_RS14940 at 3231.546 kb on - strand, within RR42_RS14940 at 3231.600 kb on - strand, within RR42_RS14940 at 3231.600 kb on - strand, within RR42_RS14940 at 3231.615 kb on + strand, within RR42_RS14940 at 3231.713 kb on + strand, within RR42_RS14940 at 3231.752 kb on - strand, within RR42_RS14940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,228,220 - RR42_RS14925 0.38 -0.3 3,228,220 - RR42_RS14925 0.38 -0.3 3,228,220 - RR42_RS14925 0.38 -0.8 3,228,228 + RR42_RS14925 0.39 +2.3 3,228,476 - RR42_RS14925 0.76 -0.5 3,228,769 + RR42_RS14930 0.20 -0.1 3,228,769 + RR42_RS14930 0.20 -1.5 3,228,770 - RR42_RS14930 0.20 -0.2 3,229,090 + +0.3 3,229,090 + -0.3 3,229,244 - -0.5 3,229,579 + RR42_RS14935 0.26 +0.5 3,229,579 + RR42_RS14935 0.26 -1.5 3,230,254 + RR42_RS14935 0.63 +0.4 3,230,254 + RR42_RS14935 0.63 -0.7 3,230,494 + RR42_RS14935 0.77 +0.6 3,230,551 + RR42_RS14935 0.80 +0.1 3,230,551 + RR42_RS14935 0.80 +2.3 3,230,552 - RR42_RS14935 0.80 +0.1 3,230,554 + RR42_RS14935 0.80 +0.7 3,230,825 - +1.3 3,230,930 - -1.0 3,230,930 - -0.2 3,230,949 + -0.3 3,230,949 + -1.1 3,230,950 - +0.3 3,230,985 - +0.7 3,230,985 - -0.1 3,231,112 + -1.1 3,231,191 + RR42_RS14940 0.13 +1.3 3,231,192 - RR42_RS14940 0.13 +0.5 3,231,192 - RR42_RS14940 0.13 +1.3 3,231,269 + RR42_RS14940 0.18 -1.4 3,231,270 - RR42_RS14940 0.18 -2.2 3,231,411 - RR42_RS14940 0.27 +0.7 3,231,462 - RR42_RS14940 0.30 +0.4 3,231,462 - RR42_RS14940 0.30 +0.4 3,231,545 + RR42_RS14940 0.36 -0.2 3,231,545 + RR42_RS14940 0.36 +0.3 3,231,546 - RR42_RS14940 0.36 -0.4 3,231,546 - RR42_RS14940 0.36 -0.1 3,231,600 - RR42_RS14940 0.39 +2.3 3,231,600 - RR42_RS14940 0.39 -1.3 3,231,615 + RR42_RS14940 0.40 +0.9 3,231,713 + RR42_RS14940 0.46 -0.1 3,231,752 - RR42_RS14940 0.49 -0.5
Or see this region's nucleotide sequence