Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14850 and RR42_RS14855 are separated by 107 nucleotides RR42_RS14855 and RR42_RS14860 are separated by 22 nucleotides RR42_RS14860 and RR42_RS14865 are separated by 209 nucleotides RR42_RS14865 and RR42_RS14870 are separated by 25 nucleotides
RR42_RS14850: RR42_RS14850 - 4-hydroxyphenylacetate permease, at 3,200,571 to 3,201,854
_RS14850
RR42_RS14855: RR42_RS14855 - monooxygenase, at 3,201,962 to 3,203,653
_RS14855
RR42_RS14860: RR42_RS14860 - hypothetical protein, at 3,203,676 to 3,203,870
_RS14860
RR42_RS14865: RR42_RS14865 - hypothetical protein, at 3,204,080 to 3,204,562
_RS14865
RR42_RS14870: RR42_RS14870 - hypothetical protein, at 3,204,588 to 3,205,784
_RS14870
Position (kb)
3201
3202
3203
3204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3201.052 kb on - strand, within RR42_RS14850 at 3201.306 kb on + strand, within RR42_RS14850 at 3201.389 kb on + strand, within RR42_RS14850 at 3201.424 kb on + strand, within RR42_RS14850 at 3201.424 kb on + strand, within RR42_RS14850 at 3201.424 kb on + strand, within RR42_RS14850 at 3201.425 kb on - strand, within RR42_RS14850 at 3201.425 kb on - strand, within RR42_RS14850 at 3201.425 kb on - strand, within RR42_RS14850 at 3201.541 kb on - strand, within RR42_RS14850 at 3201.597 kb on + strand, within RR42_RS14850 at 3201.597 kb on + strand, within RR42_RS14850 at 3201.597 kb on + strand, within RR42_RS14850 at 3201.756 kb on - strand at 3201.769 kb on - strand at 3201.998 kb on + strand at 3202.001 kb on + strand at 3202.008 kb on - strand at 3202.364 kb on + strand, within RR42_RS14855 at 3202.365 kb on - strand, within RR42_RS14855 at 3202.496 kb on + strand, within RR42_RS14855 at 3202.587 kb on - strand, within RR42_RS14855 at 3202.804 kb on - strand, within RR42_RS14855 at 3202.804 kb on - strand, within RR42_RS14855 at 3202.887 kb on - strand, within RR42_RS14855 at 3202.887 kb on - strand, within RR42_RS14855 at 3203.035 kb on - strand, within RR42_RS14855 at 3203.140 kb on - strand, within RR42_RS14855 at 3203.225 kb on + strand, within RR42_RS14855 at 3203.225 kb on + strand, within RR42_RS14855 at 3203.226 kb on - strand, within RR42_RS14855 at 3203.330 kb on + strand, within RR42_RS14855 at 3203.330 kb on + strand, within RR42_RS14855 at 3203.369 kb on + strand, within RR42_RS14855 at 3203.369 kb on + strand, within RR42_RS14855 at 3203.559 kb on - strand at 3203.698 kb on + strand, within RR42_RS14860 at 3203.725 kb on - strand, within RR42_RS14860 at 3203.725 kb on - strand, within RR42_RS14860 at 3203.858 kb on + strand at 3203.859 kb on - strand at 3203.868 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,201,052 - RR42_RS14850 0.37 -0.3 3,201,306 + RR42_RS14850 0.57 +1.3 3,201,389 + RR42_RS14850 0.64 +0.8 3,201,424 + RR42_RS14850 0.66 -0.4 3,201,424 + RR42_RS14850 0.66 -0.3 3,201,424 + RR42_RS14850 0.66 -0.0 3,201,425 - RR42_RS14850 0.67 +0.4 3,201,425 - RR42_RS14850 0.67 +0.1 3,201,425 - RR42_RS14850 0.67 -0.2 3,201,541 - RR42_RS14850 0.76 -0.5 3,201,597 + RR42_RS14850 0.80 +0.2 3,201,597 + RR42_RS14850 0.80 +2.4 3,201,597 + RR42_RS14850 0.80 -1.3 3,201,756 - +0.4 3,201,769 - +0.9 3,201,998 + -0.7 3,202,001 + -2.5 3,202,008 - +1.2 3,202,364 + RR42_RS14855 0.24 -1.0 3,202,365 - RR42_RS14855 0.24 +0.3 3,202,496 + RR42_RS14855 0.32 -2.8 3,202,587 - RR42_RS14855 0.37 -0.0 3,202,804 - RR42_RS14855 0.50 +0.1 3,202,804 - RR42_RS14855 0.50 +0.6 3,202,887 - RR42_RS14855 0.55 +0.1 3,202,887 - RR42_RS14855 0.55 -1.5 3,203,035 - RR42_RS14855 0.63 -0.9 3,203,140 - RR42_RS14855 0.70 +2.6 3,203,225 + RR42_RS14855 0.75 +0.1 3,203,225 + RR42_RS14855 0.75 -1.4 3,203,226 - RR42_RS14855 0.75 +0.3 3,203,330 + RR42_RS14855 0.81 -0.3 3,203,330 + RR42_RS14855 0.81 +0.3 3,203,369 + RR42_RS14855 0.83 +0.2 3,203,369 + RR42_RS14855 0.83 +0.9 3,203,559 - -1.9 3,203,698 + RR42_RS14860 0.11 -0.7 3,203,725 - RR42_RS14860 0.25 -0.4 3,203,725 - RR42_RS14860 0.25 +0.1 3,203,858 + -0.3 3,203,859 - -0.7 3,203,868 + -0.3
Or see this region's nucleotide sequence