Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14780 and RR42_RS14785 are separated by 54 nucleotides RR42_RS14785 and RR42_RS14790 are separated by 66 nucleotides RR42_RS14790 and RR42_RS14795 are separated by 159 nucleotides
RR42_RS14780: RR42_RS14780 - GTPase RsgA, at 3,184,768 to 3,185,796
_RS14780
RR42_RS14785: RR42_RS14785 - hypothetical protein, at 3,185,851 to 3,186,159
_RS14785
RR42_RS14790: RR42_RS14790 - cobalamin biosynthesis protein CobD, at 3,186,226 to 3,187,218
_RS14790
RR42_RS14795: RR42_RS14795 - quercetin 2,3-dioxygenase, at 3,187,378 to 3,188,076
_RS14795
Position (kb)
3186
3187
3188 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3185.794 kb on + strand at 3185.858 kb on + strand at 3185.859 kb on - strand at 3185.864 kb on + strand at 3185.943 kb on + strand, within RR42_RS14785 at 3185.944 kb on - strand, within RR42_RS14785 at 3186.042 kb on - strand, within RR42_RS14785 at 3186.042 kb on - strand, within RR42_RS14785 at 3186.042 kb on - strand, within RR42_RS14785 at 3186.042 kb on - strand, within RR42_RS14785 at 3186.318 kb on + strand at 3186.319 kb on - strand at 3186.319 kb on - strand at 3186.392 kb on + strand, within RR42_RS14790 at 3186.392 kb on + strand, within RR42_RS14790 at 3186.392 kb on + strand, within RR42_RS14790 at 3186.393 kb on - strand, within RR42_RS14790 at 3186.393 kb on - strand, within RR42_RS14790 at 3186.551 kb on + strand, within RR42_RS14790 at 3186.551 kb on + strand, within RR42_RS14790 at 3186.552 kb on - strand, within RR42_RS14790 at 3186.626 kb on + strand, within RR42_RS14790 at 3186.627 kb on - strand, within RR42_RS14790 at 3186.702 kb on - strand, within RR42_RS14790 at 3186.702 kb on - strand, within RR42_RS14790 at 3186.716 kb on + strand, within RR42_RS14790 at 3186.717 kb on - strand, within RR42_RS14790 at 3186.717 kb on - strand, within RR42_RS14790 at 3186.717 kb on - strand, within RR42_RS14790 at 3186.963 kb on - strand, within RR42_RS14790 at 3186.963 kb on - strand, within RR42_RS14790 at 3187.019 kb on + strand, within RR42_RS14790 at 3187.020 kb on - strand, within RR42_RS14790 at 3187.020 kb on - strand, within RR42_RS14790 at 3187.145 kb on + strand at 3187.145 kb on + strand at 3187.146 kb on - strand at 3187.146 kb on - strand at 3187.207 kb on + strand at 3187.208 kb on - strand at 3187.208 kb on - strand at 3187.280 kb on + strand at 3187.281 kb on - strand at 3187.284 kb on + strand at 3187.285 kb on - strand at 3187.297 kb on + strand at 3187.297 kb on + strand at 3187.298 kb on - strand at 3187.298 kb on - strand at 3187.302 kb on + strand at 3187.303 kb on - strand at 3187.518 kb on - strand, within RR42_RS14795 at 3187.518 kb on - strand, within RR42_RS14795 at 3187.518 kb on - strand, within RR42_RS14795 at 3187.580 kb on + strand, within RR42_RS14795 at 3187.767 kb on - strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.878 kb on - strand, within RR42_RS14795 at 3187.878 kb on - strand, within RR42_RS14795 at 3187.878 kb on - strand, within RR42_RS14795 at 3187.988 kb on + strand, within RR42_RS14795 at 3187.988 kb on + strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.992 kb on - strand, within RR42_RS14795 at 3188.010 kb on + strand at 3188.010 kb on + strand at 3188.010 kb on + strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.015 kb on + strand at 3188.042 kb on + strand at 3188.043 kb on - strand at 3188.120 kb on + strand at 3188.121 kb on - strand at 3188.180 kb on - strand at 3188.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,185,794 + -0.2 3,185,858 + +0.5 3,185,859 - +1.0 3,185,864 + +2.9 3,185,943 + RR42_RS14785 0.30 -0.4 3,185,944 - RR42_RS14785 0.30 +1.1 3,186,042 - RR42_RS14785 0.62 -0.5 3,186,042 - RR42_RS14785 0.62 -1.2 3,186,042 - RR42_RS14785 0.62 -0.1 3,186,042 - RR42_RS14785 0.62 +0.5 3,186,318 + -0.2 3,186,319 - -1.0 3,186,319 - -1.3 3,186,392 + RR42_RS14790 0.17 -1.0 3,186,392 + RR42_RS14790 0.17 -1.1 3,186,392 + RR42_RS14790 0.17 -1.9 3,186,393 - RR42_RS14790 0.17 -0.3 3,186,393 - RR42_RS14790 0.17 -1.5 3,186,551 + RR42_RS14790 0.33 -3.7 3,186,551 + RR42_RS14790 0.33 -2.0 3,186,552 - RR42_RS14790 0.33 -2.2 3,186,626 + RR42_RS14790 0.40 +0.5 3,186,627 - RR42_RS14790 0.40 -1.5 3,186,702 - RR42_RS14790 0.48 -3.0 3,186,702 - RR42_RS14790 0.48 -1.1 3,186,716 + RR42_RS14790 0.49 -1.7 3,186,717 - RR42_RS14790 0.49 -1.0 3,186,717 - RR42_RS14790 0.49 -1.9 3,186,717 - RR42_RS14790 0.49 -0.7 3,186,963 - RR42_RS14790 0.74 -1.6 3,186,963 - RR42_RS14790 0.74 -1.6 3,187,019 + RR42_RS14790 0.80 -0.4 3,187,020 - RR42_RS14790 0.80 -1.5 3,187,020 - RR42_RS14790 0.80 -1.1 3,187,145 + +0.3 3,187,145 + -1.7 3,187,146 - -1.0 3,187,146 - -0.6 3,187,207 + +5.4 3,187,208 - -0.5 3,187,208 - -2.1 3,187,280 + -1.0 3,187,281 - -0.8 3,187,284 + -2.9 3,187,285 - -0.3 3,187,297 + -3.3 3,187,297 + +0.3 3,187,298 - +0.3 3,187,298 - -0.8 3,187,302 + -0.4 3,187,303 - +0.2 3,187,518 - RR42_RS14795 0.20 +1.3 3,187,518 - RR42_RS14795 0.20 +1.4 3,187,518 - RR42_RS14795 0.20 -0.1 3,187,580 + RR42_RS14795 0.29 -0.3 3,187,767 - RR42_RS14795 0.56 -0.4 3,187,877 + RR42_RS14795 0.71 -0.3 3,187,877 + RR42_RS14795 0.71 +0.9 3,187,877 + RR42_RS14795 0.71 -0.4 3,187,877 + RR42_RS14795 0.71 -2.2 3,187,877 + RR42_RS14795 0.71 -0.2 3,187,878 - RR42_RS14795 0.72 -0.6 3,187,878 - RR42_RS14795 0.72 -1.9 3,187,878 - RR42_RS14795 0.72 -0.3 3,187,988 + RR42_RS14795 0.87 +0.4 3,187,988 + RR42_RS14795 0.87 -0.0 3,187,989 - RR42_RS14795 0.87 +0.9 3,187,989 - RR42_RS14795 0.87 +0.8 3,187,989 - RR42_RS14795 0.87 +0.1 3,187,989 - RR42_RS14795 0.87 +0.5 3,187,989 - RR42_RS14795 0.87 +0.8 3,187,989 - RR42_RS14795 0.87 +0.4 3,187,989 - RR42_RS14795 0.87 -0.7 3,187,992 - RR42_RS14795 0.88 -0.6 3,188,010 + +1.0 3,188,010 + -1.7 3,188,010 + +0.4 3,188,011 - +0.1 3,188,011 - +0.0 3,188,011 - +0.5 3,188,011 - -0.4 3,188,011 - -0.0 3,188,011 - -0.4 3,188,015 + +1.0 3,188,042 + -1.3 3,188,043 - +0.1 3,188,120 + -0.1 3,188,121 - -0.3 3,188,180 - +0.1 3,188,200 - -0.2
Or see this region's nucleotide sequence