Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14400 and RR42_RS14405 are separated by 295 nucleotides RR42_RS14405 and RR42_RS14410 overlap by 1 nucleotides RR42_RS14410 and RR42_RS14415 overlap by 4 nucleotides
RR42_RS14400: RR42_RS14400 - isopropylmalate isomerase, at 3,096,533 to 3,097,942
_RS14400
RR42_RS14405: RR42_RS14405 - leucine/isoleucine/valine transporter ATP-binding subunit, at 3,098,238 to 3,098,939
_RS14405
RR42_RS14410: RR42_RS14410 - leucine/isoleucine/valine transporter ATP-binding subunit, at 3,098,939 to 3,099,718
_RS14410
RR42_RS14415: RR42_RS14415 - leucine/isoleucine/valine transporter permease subunit, at 3,099,715 to 3,100,989
_RS14415
Position (kb)
3098
3099
3100 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3098.133 kb on + strand at 3098.134 kb on - strand at 3098.251 kb on + strand at 3098.252 kb on - strand at 3098.330 kb on - strand, within RR42_RS14405 at 3098.590 kb on + strand, within RR42_RS14405 at 3098.591 kb on - strand, within RR42_RS14405 at 3098.591 kb on - strand, within RR42_RS14405 at 3098.591 kb on - strand, within RR42_RS14405 at 3098.915 kb on + strand at 3098.955 kb on + strand at 3098.956 kb on - strand at 3099.240 kb on + strand, within RR42_RS14410 at 3099.241 kb on - strand, within RR42_RS14410 at 3099.543 kb on + strand, within RR42_RS14410 at 3099.543 kb on + strand, within RR42_RS14410 at 3099.544 kb on - strand, within RR42_RS14410 at 3100.176 kb on - strand, within RR42_RS14415 at 3100.206 kb on - strand, within RR42_RS14415 at 3100.392 kb on - strand, within RR42_RS14415 at 3100.511 kb on + strand, within RR42_RS14415 at 3100.511 kb on + strand, within RR42_RS14415 at 3100.511 kb on + strand, within RR42_RS14415 at 3100.512 kb on - strand, within RR42_RS14415 at 3100.518 kb on - strand, within RR42_RS14415 at 3100.518 kb on - strand, within RR42_RS14415 at 3100.532 kb on + strand, within RR42_RS14415 at 3100.532 kb on + strand, within RR42_RS14415 at 3100.533 kb on - strand, within RR42_RS14415 at 3100.533 kb on - strand, within RR42_RS14415 at 3100.545 kb on - strand, within RR42_RS14415 at 3100.545 kb on - strand, within RR42_RS14415 at 3100.569 kb on - strand, within RR42_RS14415 at 3100.607 kb on + strand, within RR42_RS14415 at 3100.607 kb on + strand, within RR42_RS14415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,098,133 + +0.3 3,098,134 - +0.4 3,098,251 + -0.4 3,098,252 - +0.5 3,098,330 - RR42_RS14405 0.13 +2.5 3,098,590 + RR42_RS14405 0.50 -0.6 3,098,591 - RR42_RS14405 0.50 -1.3 3,098,591 - RR42_RS14405 0.50 +0.6 3,098,591 - RR42_RS14405 0.50 +0.5 3,098,915 + +1.1 3,098,955 + +1.0 3,098,956 - +0.3 3,099,240 + RR42_RS14410 0.39 +1.5 3,099,241 - RR42_RS14410 0.39 -0.7 3,099,543 + RR42_RS14410 0.77 +0.5 3,099,543 + RR42_RS14410 0.77 +0.5 3,099,544 - RR42_RS14410 0.78 -0.2 3,100,176 - RR42_RS14415 0.36 +0.7 3,100,206 - RR42_RS14415 0.39 -0.9 3,100,392 - RR42_RS14415 0.53 +0.5 3,100,511 + RR42_RS14415 0.62 -0.0 3,100,511 + RR42_RS14415 0.62 +1.0 3,100,511 + RR42_RS14415 0.62 -0.8 3,100,512 - RR42_RS14415 0.63 +1.4 3,100,518 - RR42_RS14415 0.63 -0.3 3,100,518 - RR42_RS14415 0.63 +0.7 3,100,532 + RR42_RS14415 0.64 -0.1 3,100,532 + RR42_RS14415 0.64 -0.5 3,100,533 - RR42_RS14415 0.64 -1.1 3,100,533 - RR42_RS14415 0.64 +0.2 3,100,545 - RR42_RS14415 0.65 -0.0 3,100,545 - RR42_RS14415 0.65 +0.5 3,100,569 - RR42_RS14415 0.67 +0.5 3,100,607 + RR42_RS14415 0.70 +3.0 3,100,607 + RR42_RS14415 0.70 +0.1
Or see this region's nucleotide sequence