Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13035 and RR42_RS13040 are separated by 110 nucleotides RR42_RS13040 and RR42_RS13045 are separated by 99 nucleotides RR42_RS13045 and RR42_RS13050 are separated by 174 nucleotides
RR42_RS13035: RR42_RS13035 - amino acid acetyltransferase, at 2,808,618 to 2,810,054
_RS13035
RR42_RS13040: RR42_RS13040 - iron transporter, at 2,810,165 to 2,810,440
_RS13040
RR42_RS13045: RR42_RS13045 - ribose-5-phosphate isomerase A, at 2,810,540 to 2,811,226
_RS13045
RR42_RS13050: RR42_RS13050 - transaldolase, at 2,811,401 to 2,812,339
_RS13050
Position (kb)
2810
2811 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2810.094 kb on + strand at 2810.094 kb on + strand at 2810.095 kb on - strand at 2810.095 kb on - strand at 2810.099 kb on + strand at 2810.137 kb on + strand at 2810.138 kb on - strand at 2810.381 kb on + strand, within RR42_RS13040 at 2810.381 kb on + strand, within RR42_RS13040 at 2810.382 kb on - strand, within RR42_RS13040 at 2810.417 kb on + strand at 2810.417 kb on + strand at 2810.418 kb on - strand at 2810.418 kb on - strand at 2810.418 kb on - strand at 2811.261 kb on - strand at 2811.308 kb on + strand at 2811.309 kb on + strand at 2811.309 kb on + strand at 2811.310 kb on - strand at 2811.310 kb on - strand at 2811.382 kb on + strand at 2811.382 kb on + strand at 2811.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,810,094 + -0.3 2,810,094 + -0.3 2,810,095 - -0.0 2,810,095 - -0.1 2,810,099 + -0.0 2,810,137 + -0.5 2,810,138 - +0.3 2,810,381 + RR42_RS13040 0.78 -2.2 2,810,381 + RR42_RS13040 0.78 -1.5 2,810,382 - RR42_RS13040 0.79 -1.8 2,810,417 + -0.6 2,810,417 + +2.0 2,810,418 - -0.4 2,810,418 - +1.4 2,810,418 - +0.7 2,811,261 - -0.3 2,811,308 + +2.9 2,811,309 + -0.0 2,811,309 + +2.9 2,811,310 - +1.5 2,811,310 - +0.2 2,811,382 + +0.9 2,811,382 + +0.4 2,811,383 - +1.5
Or see this region's nucleotide sequence