Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12585 and RR42_RS12590 are separated by 256 nucleotides RR42_RS12590 and RR42_RS12595 are separated by 31 nucleotides RR42_RS12595 and RR42_RS12600 are separated by 3 nucleotides RR42_RS12600 and RR42_RS12605 are separated by 74 nucleotides
RR42_RS12585: RR42_RS12585 - DNA helicase, at 2,704,554 to 2,705,945
_RS12585
RR42_RS12590: RR42_RS12590 - 50S ribosomal protein L9, at 2,706,202 to 2,706,654
_RS12590
RR42_RS12595: RR42_RS12595 - 30S ribosomal protein S18, at 2,706,686 to 2,706,964
_RS12595
RR42_RS12600: RR42_RS12600 - primosomal replication protein N, at 2,706,968 to 2,707,267
_RS12600
RR42_RS12605: RR42_RS12605 - 30S ribosomal protein S6, at 2,707,342 to 2,707,716
_RS12605
Position (kb)
2706
2707 Strain fitness (log2 ratio)
-2
-1
0
1 at 2705.958 kb on + strand at 2705.958 kb on + strand at 2705.958 kb on + strand at 2705.959 kb on - strand at 2706.049 kb on + strand at 2706.049 kb on + strand at 2706.050 kb on - strand at 2706.203 kb on + strand at 2706.203 kb on + strand at 2706.581 kb on + strand, within RR42_RS12590 at 2706.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,705,958 + -2.2 2,705,958 + -1.1 2,705,958 + +1.6 2,705,959 - -1.6 2,706,049 + +0.2 2,706,049 + +0.8 2,706,050 - +0.3 2,706,203 + +0.6 2,706,203 + -2.0 2,706,581 + RR42_RS12590 0.84 -1.6 2,706,708 + -1.4
Or see this region's nucleotide sequence