Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12445 and RR42_RS12450 are separated by 109 nucleotides RR42_RS12450 and RR42_RS12455 are separated by 209 nucleotides RR42_RS12455 and RR42_RS12460 are separated by 196 nucleotides
RR42_RS12445: RR42_RS12445 - hypothetical protein, at 2,674,723 to 2,675,238
_RS12445
RR42_RS12450: RR42_RS12450 - hypothetical protein, at 2,675,348 to 2,675,716
_RS12450
RR42_RS12455: RR42_RS12455 - protein disaggregation chaperone, at 2,675,926 to 2,678,514
_RS12455
RR42_RS12460: RR42_RS12460 - oxidoreductase, at 2,678,711 to 2,679,640
_RS12460
Position (kb)
2675
2676
2677
2678
2679 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2675.210 kb on - strand at 2675.310 kb on + strand at 2675.310 kb on + strand at 2675.311 kb on - strand at 2675.321 kb on + strand at 2675.321 kb on + strand at 2675.322 kb on - strand at 2675.366 kb on + strand at 2675.492 kb on + strand, within RR42_RS12450 at 2675.537 kb on + strand, within RR42_RS12450 at 2675.558 kb on + strand, within RR42_RS12450 at 2675.576 kb on + strand, within RR42_RS12450 at 2675.642 kb on + strand, within RR42_RS12450 at 2675.681 kb on + strand at 2675.682 kb on - strand at 2675.682 kb on - strand at 2675.682 kb on - strand at 2675.682 kb on - strand at 2675.687 kb on + strand at 2675.687 kb on + strand at 2675.687 kb on + strand at 2675.688 kb on - strand at 2675.688 kb on - strand at 2675.699 kb on + strand at 2675.836 kb on - strand at 2675.846 kb on - strand at 2675.846 kb on - strand at 2675.951 kb on + strand at 2675.952 kb on - strand at 2675.952 kb on - strand at 2675.952 kb on - strand at 2675.967 kb on - strand at 2676.086 kb on + strand at 2676.198 kb on + strand, within RR42_RS12455 at 2676.287 kb on - strand, within RR42_RS12455 at 2676.495 kb on - strand, within RR42_RS12455 at 2676.548 kb on + strand, within RR42_RS12455 at 2676.794 kb on - strand, within RR42_RS12455 at 2676.812 kb on + strand, within RR42_RS12455 at 2677.154 kb on + strand, within RR42_RS12455 at 2677.155 kb on - strand, within RR42_RS12455 at 2677.378 kb on - strand, within RR42_RS12455 at 2677.379 kb on + strand, within RR42_RS12455 at 2677.379 kb on + strand, within RR42_RS12455 at 2677.379 kb on + strand, within RR42_RS12455 at 2677.380 kb on - strand, within RR42_RS12455 at 2677.448 kb on + strand, within RR42_RS12455 at 2677.449 kb on - strand, within RR42_RS12455 at 2677.449 kb on - strand, within RR42_RS12455 at 2677.553 kb on + strand, within RR42_RS12455 at 2677.690 kb on - strand, within RR42_RS12455 at 2678.028 kb on - strand, within RR42_RS12455 at 2678.028 kb on - strand, within RR42_RS12455 at 2678.433 kb on - strand at 2678.517 kb on - strand at 2678.626 kb on - strand at 2678.685 kb on + strand at 2678.968 kb on + strand, within RR42_RS12460 at 2678.969 kb on - strand, within RR42_RS12460 at 2679.039 kb on + strand, within RR42_RS12460 at 2679.039 kb on + strand, within RR42_RS12460 at 2679.039 kb on + strand, within RR42_RS12460 at 2679.040 kb on - strand, within RR42_RS12460 at 2679.040 kb on - strand, within RR42_RS12460 at 2679.040 kb on - strand, within RR42_RS12460 at 2679.040 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.073 kb on - strand, within RR42_RS12460 at 2679.139 kb on - strand, within RR42_RS12460 at 2679.145 kb on + strand, within RR42_RS12460 at 2679.146 kb on - strand, within RR42_RS12460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,675,210 - +1.3 2,675,310 + +1.1 2,675,310 + +0.9 2,675,311 - +1.1 2,675,321 + +0.2 2,675,321 + -1.1 2,675,322 - +1.3 2,675,366 + +1.2 2,675,492 + RR42_RS12450 0.39 -0.2 2,675,537 + RR42_RS12450 0.51 -0.4 2,675,558 + RR42_RS12450 0.57 -0.1 2,675,576 + RR42_RS12450 0.62 +0.7 2,675,642 + RR42_RS12450 0.80 -0.4 2,675,681 + -0.6 2,675,682 - -0.4 2,675,682 - -0.4 2,675,682 - -1.0 2,675,682 - -0.3 2,675,687 + +0.3 2,675,687 + -0.1 2,675,687 + -1.5 2,675,688 - +0.4 2,675,688 - +2.3 2,675,699 + +0.7 2,675,836 - +0.5 2,675,846 - +0.1 2,675,846 - +1.3 2,675,951 + +0.4 2,675,952 - +1.5 2,675,952 - +0.0 2,675,952 - +0.2 2,675,967 - -0.7 2,676,086 + -0.5 2,676,198 + RR42_RS12455 0.11 +1.1 2,676,287 - RR42_RS12455 0.14 -0.2 2,676,495 - RR42_RS12455 0.22 -0.0 2,676,548 + RR42_RS12455 0.24 +0.3 2,676,794 - RR42_RS12455 0.34 -1.2 2,676,812 + RR42_RS12455 0.34 -0.0 2,677,154 + RR42_RS12455 0.47 -0.0 2,677,155 - RR42_RS12455 0.47 +0.4 2,677,378 - RR42_RS12455 0.56 +0.2 2,677,379 + RR42_RS12455 0.56 +0.2 2,677,379 + RR42_RS12455 0.56 +1.9 2,677,379 + RR42_RS12455 0.56 +1.4 2,677,380 - RR42_RS12455 0.56 +0.3 2,677,448 + RR42_RS12455 0.59 +0.6 2,677,449 - RR42_RS12455 0.59 -0.0 2,677,449 - RR42_RS12455 0.59 +0.0 2,677,553 + RR42_RS12455 0.63 +0.3 2,677,690 - RR42_RS12455 0.68 +0.9 2,678,028 - RR42_RS12455 0.81 +0.9 2,678,028 - RR42_RS12455 0.81 +0.6 2,678,433 - +0.6 2,678,517 - +1.0 2,678,626 - +0.5 2,678,685 + -0.6 2,678,968 + RR42_RS12460 0.28 +0.6 2,678,969 - RR42_RS12460 0.28 +0.3 2,679,039 + RR42_RS12460 0.35 +1.3 2,679,039 + RR42_RS12460 0.35 +0.6 2,679,039 + RR42_RS12460 0.35 +1.5 2,679,040 - RR42_RS12460 0.35 +0.7 2,679,040 - RR42_RS12460 0.35 +1.4 2,679,040 - RR42_RS12460 0.35 -0.5 2,679,040 - RR42_RS12460 0.35 +0.4 2,679,073 - RR42_RS12460 0.39 +0.5 2,679,073 - RR42_RS12460 0.39 +0.1 2,679,073 - RR42_RS12460 0.39 +1.4 2,679,073 - RR42_RS12460 0.39 -0.5 2,679,073 - RR42_RS12460 0.39 +0.5 2,679,139 - RR42_RS12460 0.46 -2.3 2,679,145 + RR42_RS12460 0.47 +0.9 2,679,146 - RR42_RS12460 0.47 -0.3
Or see this region's nucleotide sequence