Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12015 and RR42_RS12020 are separated by 125 nucleotides RR42_RS12020 and RR42_RS12025 are separated by 76 nucleotides RR42_RS12025 and RR42_RS12030 are separated by 82 nucleotides
RR42_RS12015: RR42_RS12015 - dihydrolipoamide acyltransferase, at 2,576,324 to 2,576,809
_RS12015
RR42_RS12020: RR42_RS12020 - GCN5 family acetyltransferase, at 2,576,935 to 2,577,471
_RS12020
RR42_RS12025: RR42_RS12025 - hypothetical protein, at 2,577,548 to 2,578,123
_RS12025
RR42_RS12030: RR42_RS12030 - DNA repair protein RadA, at 2,578,206 to 2,579,567
_RS12030
Position (kb)
2577
2578
2579 Strain fitness (log2 ratio)
-2
-1
0
1 at 2576.818 kb on - strand at 2576.856 kb on - strand at 2576.856 kb on - strand at 2576.867 kb on + strand at 2576.867 kb on + strand at 2576.867 kb on + strand at 2576.868 kb on - strand at 2576.868 kb on - strand at 2577.158 kb on + strand, within RR42_RS12020 at 2577.158 kb on + strand, within RR42_RS12020 at 2577.158 kb on + strand, within RR42_RS12020 at 2577.588 kb on + strand at 2577.589 kb on - strand at 2577.841 kb on + strand, within RR42_RS12025 at 2577.851 kb on + strand, within RR42_RS12025 at 2577.851 kb on + strand, within RR42_RS12025 at 2577.852 kb on - strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.961 kb on - strand, within RR42_RS12025 at 2577.961 kb on - strand, within RR42_RS12025 at 2577.987 kb on + strand, within RR42_RS12025 at 2577.988 kb on - strand, within RR42_RS12025 at 2577.988 kb on - strand, within RR42_RS12025 at 2578.162 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,576,818 - -1.5 2,576,856 - +0.9 2,576,856 - +0.8 2,576,867 + -1.9 2,576,867 + -0.3 2,576,867 + -0.4 2,576,868 - +0.9 2,576,868 - -0.1 2,577,158 + RR42_RS12020 0.42 -0.1 2,577,158 + RR42_RS12020 0.42 +1.0 2,577,158 + RR42_RS12020 0.42 +1.0 2,577,588 + +0.8 2,577,589 - -0.5 2,577,841 + RR42_RS12025 0.51 -0.3 2,577,851 + RR42_RS12025 0.53 -0.8 2,577,851 + RR42_RS12025 0.53 -1.0 2,577,852 - RR42_RS12025 0.53 -0.7 2,577,903 + RR42_RS12025 0.62 -1.0 2,577,903 + RR42_RS12025 0.62 +1.4 2,577,903 + RR42_RS12025 0.62 -0.0 2,577,903 + RR42_RS12025 0.62 -0.6 2,577,903 + RR42_RS12025 0.62 -0.1 2,577,903 + RR42_RS12025 0.62 -0.4 2,577,903 + RR42_RS12025 0.62 +0.8 2,577,904 - RR42_RS12025 0.62 -0.3 2,577,904 - RR42_RS12025 0.62 +0.9 2,577,904 - RR42_RS12025 0.62 +0.9 2,577,904 - RR42_RS12025 0.62 +1.1 2,577,961 - RR42_RS12025 0.72 +0.9 2,577,961 - RR42_RS12025 0.72 +0.1 2,577,987 + RR42_RS12025 0.76 +1.3 2,577,988 - RR42_RS12025 0.76 -1.0 2,577,988 - RR42_RS12025 0.76 +0.1 2,578,162 - +0.3
Or see this region's nucleotide sequence