Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12005 and RR42_RS12010 are separated by 366 nucleotides RR42_RS12010 and RR42_RS12015 are separated by 74 nucleotides RR42_RS12015 and RR42_RS12020 are separated by 125 nucleotides
RR42_RS12005: RR42_RS12005 - transcriptional regulator, at 2,572,983 to 2,573,942
_RS12005
RR42_RS12010: RR42_RS12010 - ABC transporter, at 2,574,309 to 2,576,249
_RS12010
RR42_RS12015: RR42_RS12015 - dihydrolipoamide acyltransferase, at 2,576,324 to 2,576,809
_RS12015
RR42_RS12020: RR42_RS12020 - GCN5 family acetyltransferase, at 2,576,935 to 2,577,471
_RS12020
Position (kb)
2574
2575
2576
2577 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2573.365 kb on + strand, within RR42_RS12005 at 2573.365 kb on + strand, within RR42_RS12005 at 2573.365 kb on + strand, within RR42_RS12005 at 2573.366 kb on - strand, within RR42_RS12005 at 2573.366 kb on - strand, within RR42_RS12005 at 2573.366 kb on - strand, within RR42_RS12005 at 2573.371 kb on + strand, within RR42_RS12005 at 2573.372 kb on - strand, within RR42_RS12005 at 2573.486 kb on + strand, within RR42_RS12005 at 2573.487 kb on - strand, within RR42_RS12005 at 2573.878 kb on + strand at 2573.879 kb on - strand at 2573.987 kb on + strand at 2573.988 kb on - strand at 2573.988 kb on - strand at 2574.073 kb on - strand at 2574.076 kb on + strand at 2574.142 kb on + strand at 2574.142 kb on + strand at 2574.143 kb on - strand at 2574.197 kb on + strand at 2574.198 kb on - strand at 2574.208 kb on + strand at 2574.208 kb on + strand at 2574.208 kb on + strand at 2574.208 kb on + strand at 2574.208 kb on + strand at 2574.208 kb on - strand at 2574.209 kb on - strand at 2574.209 kb on - strand at 2574.246 kb on + strand at 2574.258 kb on + strand at 2574.259 kb on - strand at 2574.328 kb on + strand at 2574.329 kb on - strand at 2574.329 kb on - strand at 2574.329 kb on - strand at 2574.329 kb on - strand at 2574.329 kb on - strand at 2574.503 kb on + strand, within RR42_RS12010 at 2574.504 kb on - strand, within RR42_RS12010 at 2574.535 kb on + strand, within RR42_RS12010 at 2574.999 kb on + strand, within RR42_RS12010 at 2574.999 kb on + strand, within RR42_RS12010 at 2575.000 kb on - strand, within RR42_RS12010 at 2575.075 kb on + strand, within RR42_RS12010 at 2575.076 kb on - strand, within RR42_RS12010 at 2575.465 kb on - strand, within RR42_RS12010 at 2576.124 kb on + strand at 2576.124 kb on + strand at 2576.124 kb on + strand at 2576.125 kb on - strand at 2576.125 kb on - strand at 2576.331 kb on + strand at 2576.332 kb on - strand at 2576.460 kb on + strand, within RR42_RS12015 at 2576.460 kb on + strand, within RR42_RS12015 at 2576.461 kb on - strand, within RR42_RS12015 at 2576.818 kb on - strand at 2576.856 kb on - strand at 2576.856 kb on - strand at 2576.867 kb on + strand at 2576.867 kb on + strand at 2576.867 kb on + strand at 2576.868 kb on - strand at 2576.868 kb on - strand at 2577.158 kb on + strand, within RR42_RS12020 at 2577.158 kb on + strand, within RR42_RS12020 at 2577.158 kb on + strand, within RR42_RS12020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,573,365 + RR42_RS12005 0.40 +0.1 2,573,365 + RR42_RS12005 0.40 -0.3 2,573,365 + RR42_RS12005 0.40 +1.0 2,573,366 - RR42_RS12005 0.40 -2.1 2,573,366 - RR42_RS12005 0.40 -1.7 2,573,366 - RR42_RS12005 0.40 -2.1 2,573,371 + RR42_RS12005 0.40 -0.4 2,573,372 - RR42_RS12005 0.41 +0.3 2,573,486 + RR42_RS12005 0.52 +0.4 2,573,487 - RR42_RS12005 0.53 +2.8 2,573,878 + +1.6 2,573,879 - -2.3 2,573,987 + +0.0 2,573,988 - +0.1 2,573,988 - +0.1 2,574,073 - +0.1 2,574,076 + +0.5 2,574,142 + -1.1 2,574,142 + +0.7 2,574,143 - -0.3 2,574,197 + +1.3 2,574,198 - +0.0 2,574,208 + +0.4 2,574,208 + +0.0 2,574,208 + -0.5 2,574,208 + -0.0 2,574,208 + -0.2 2,574,208 - -0.2 2,574,209 - +0.3 2,574,209 - -0.3 2,574,246 + -0.9 2,574,258 + -0.5 2,574,259 - -1.5 2,574,328 + -0.3 2,574,329 - +0.2 2,574,329 - -0.2 2,574,329 - +0.8 2,574,329 - +1.6 2,574,329 - -0.7 2,574,503 + RR42_RS12010 0.10 -1.1 2,574,504 - RR42_RS12010 0.10 -0.6 2,574,535 + RR42_RS12010 0.12 +0.0 2,574,999 + RR42_RS12010 0.36 -0.4 2,574,999 + RR42_RS12010 0.36 -0.3 2,575,000 - RR42_RS12010 0.36 +0.1 2,575,075 + RR42_RS12010 0.39 +0.5 2,575,076 - RR42_RS12010 0.40 -0.7 2,575,465 - RR42_RS12010 0.60 -0.2 2,576,124 + +0.8 2,576,124 + +0.3 2,576,124 + -0.3 2,576,125 - -1.7 2,576,125 - -0.3 2,576,331 + -1.7 2,576,332 - +2.3 2,576,460 + RR42_RS12015 0.28 -0.1 2,576,460 + RR42_RS12015 0.28 -2.9 2,576,461 - RR42_RS12015 0.28 -2.1 2,576,818 - -1.5 2,576,856 - +0.9 2,576,856 - +0.8 2,576,867 + -1.9 2,576,867 + -0.3 2,576,867 + -0.4 2,576,868 - +0.9 2,576,868 - -0.1 2,577,158 + RR42_RS12020 0.42 -0.1 2,577,158 + RR42_RS12020 0.42 +1.0 2,577,158 + RR42_RS12020 0.42 +1.0
Or see this region's nucleotide sequence