Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11175 and RR42_RS11180 are separated by 4 nucleotides RR42_RS11180 and RR42_RS11185 are separated by 62 nucleotides RR42_RS11185 and RR42_RS11190 are separated by 144 nucleotides
RR42_RS11175: RR42_RS11175 - BolA family transcriptional regulator, at 2,387,476 to 2,387,790
_RS11175
RR42_RS11180: RR42_RS11180 - peptidylprolyl isomerase, at 2,387,795 to 2,388,589
_RS11180
RR42_RS11185: RR42_RS11185 - TetR family transcriptional regulator, at 2,388,652 to 2,389,299
_RS11185
RR42_RS11190: RR42_RS11190 - acyl-CoA dehydrogenase, at 2,389,444 to 2,391,066
_RS11190
Position (kb)
2388
2389
2390 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2387.668 kb on + strand, within RR42_RS11175 at 2387.668 kb on + strand, within RR42_RS11175 at 2387.668 kb on + strand, within RR42_RS11175 at 2387.668 kb on + strand, within RR42_RS11175 at 2387.669 kb on - strand, within RR42_RS11175 at 2388.101 kb on + strand, within RR42_RS11180 at 2388.101 kb on + strand, within RR42_RS11180 at 2388.102 kb on - strand, within RR42_RS11180 at 2388.147 kb on - strand, within RR42_RS11180 at 2388.186 kb on - strand, within RR42_RS11180 at 2388.347 kb on + strand, within RR42_RS11180 at 2388.347 kb on + strand, within RR42_RS11180 at 2388.347 kb on + strand, within RR42_RS11180 at 2388.587 kb on + strand at 2388.587 kb on + strand at 2388.588 kb on - strand at 2388.588 kb on - strand at 2388.689 kb on + strand at 2388.847 kb on + strand, within RR42_RS11185 at 2388.884 kb on + strand, within RR42_RS11185 at 2388.885 kb on - strand, within RR42_RS11185 at 2388.885 kb on - strand, within RR42_RS11185 at 2388.914 kb on + strand, within RR42_RS11185 at 2388.915 kb on - strand, within RR42_RS11185 at 2388.915 kb on - strand, within RR42_RS11185 at 2388.933 kb on - strand, within RR42_RS11185 at 2388.973 kb on + strand, within RR42_RS11185 at 2388.973 kb on + strand, within RR42_RS11185 at 2388.974 kb on - strand, within RR42_RS11185 at 2389.084 kb on + strand, within RR42_RS11185 at 2389.115 kb on + strand, within RR42_RS11185 at 2389.115 kb on + strand, within RR42_RS11185 at 2389.116 kb on - strand, within RR42_RS11185 at 2389.121 kb on + strand, within RR42_RS11185 at 2389.122 kb on - strand, within RR42_RS11185 at 2389.122 kb on - strand, within RR42_RS11185 at 2389.182 kb on - strand, within RR42_RS11185 at 2389.336 kb on + strand at 2389.337 kb on - strand at 2389.474 kb on + strand at 2389.474 kb on + strand at 2389.475 kb on - strand at 2389.475 kb on - strand at 2389.475 kb on - strand at 2389.483 kb on + strand at 2389.483 kb on + strand at 2389.484 kb on - strand at 2389.769 kb on - strand, within RR42_RS11190 at 2389.783 kb on + strand, within RR42_RS11190 at 2389.783 kb on + strand, within RR42_RS11190 at 2389.784 kb on - strand, within RR42_RS11190 at 2389.784 kb on - strand, within RR42_RS11190 at 2389.784 kb on - strand, within RR42_RS11190 at 2389.784 kb on - strand, within RR42_RS11190 at 2389.785 kb on + strand, within RR42_RS11190 at 2389.785 kb on + strand, within RR42_RS11190 at 2389.785 kb on + strand, within RR42_RS11190 at 2389.785 kb on + strand, within RR42_RS11190 at 2389.785 kb on + strand, within RR42_RS11190 at 2389.785 kb on + strand, within RR42_RS11190 at 2389.785 kb on + strand, within RR42_RS11190 at 2389.786 kb on - strand, within RR42_RS11190 at 2389.786 kb on - strand, within RR42_RS11190 at 2389.786 kb on - strand, within RR42_RS11190 at 2389.827 kb on + strand, within RR42_RS11190 at 2389.828 kb on - strand, within RR42_RS11190 at 2389.828 kb on - strand, within RR42_RS11190 at 2389.909 kb on + strand, within RR42_RS11190 at 2389.910 kb on - strand, within RR42_RS11190 at 2390.051 kb on - strand, within RR42_RS11190 at 2390.210 kb on + strand, within RR42_RS11190 at 2390.211 kb on - strand, within RR42_RS11190 at 2390.211 kb on - strand, within RR42_RS11190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,387,668 + RR42_RS11175 0.61 -0.8 2,387,668 + RR42_RS11175 0.61 -0.5 2,387,668 + RR42_RS11175 0.61 -1.2 2,387,668 + RR42_RS11175 0.61 -0.1 2,387,669 - RR42_RS11175 0.61 +0.4 2,388,101 + RR42_RS11180 0.38 -3.7 2,388,101 + RR42_RS11180 0.38 -0.8 2,388,102 - RR42_RS11180 0.39 +0.2 2,388,147 - RR42_RS11180 0.44 -2.1 2,388,186 - RR42_RS11180 0.49 -1.1 2,388,347 + RR42_RS11180 0.69 -3.9 2,388,347 + RR42_RS11180 0.69 +2.2 2,388,347 + RR42_RS11180 0.69 -1.1 2,388,587 + -0.7 2,388,587 + -0.9 2,388,588 - +0.4 2,388,588 - -0.2 2,388,689 + -0.9 2,388,847 + RR42_RS11185 0.30 -0.3 2,388,884 + RR42_RS11185 0.36 -1.4 2,388,885 - RR42_RS11185 0.36 +1.3 2,388,885 - RR42_RS11185 0.36 -0.0 2,388,914 + RR42_RS11185 0.40 -0.4 2,388,915 - RR42_RS11185 0.41 +1.1 2,388,915 - RR42_RS11185 0.41 +0.5 2,388,933 - RR42_RS11185 0.43 +1.3 2,388,973 + RR42_RS11185 0.50 -0.3 2,388,973 + RR42_RS11185 0.50 +1.5 2,388,974 - RR42_RS11185 0.50 -0.3 2,389,084 + RR42_RS11185 0.67 +0.5 2,389,115 + RR42_RS11185 0.71 +0.2 2,389,115 + RR42_RS11185 0.71 -0.8 2,389,116 - RR42_RS11185 0.72 +0.3 2,389,121 + RR42_RS11185 0.72 +1.5 2,389,122 - RR42_RS11185 0.73 -0.9 2,389,122 - RR42_RS11185 0.73 -0.7 2,389,182 - RR42_RS11185 0.82 +0.2 2,389,336 + +1.5 2,389,337 - -0.2 2,389,474 + -0.6 2,389,474 + -1.1 2,389,475 - +0.3 2,389,475 - +0.5 2,389,475 - -1.0 2,389,483 + +0.5 2,389,483 + -0.6 2,389,484 - +0.8 2,389,769 - RR42_RS11190 0.20 +0.4 2,389,783 + RR42_RS11190 0.21 -0.3 2,389,783 + RR42_RS11190 0.21 +0.4 2,389,784 - RR42_RS11190 0.21 -0.8 2,389,784 - RR42_RS11190 0.21 -0.0 2,389,784 - RR42_RS11190 0.21 -1.0 2,389,784 - RR42_RS11190 0.21 -0.6 2,389,785 + RR42_RS11190 0.21 -0.6 2,389,785 + RR42_RS11190 0.21 -0.5 2,389,785 + RR42_RS11190 0.21 -1.0 2,389,785 + RR42_RS11190 0.21 +0.2 2,389,785 + RR42_RS11190 0.21 +1.7 2,389,785 + RR42_RS11190 0.21 -1.5 2,389,785 + RR42_RS11190 0.21 -0.8 2,389,786 - RR42_RS11190 0.21 -0.1 2,389,786 - RR42_RS11190 0.21 -0.8 2,389,786 - RR42_RS11190 0.21 +0.5 2,389,827 + RR42_RS11190 0.24 +0.3 2,389,828 - RR42_RS11190 0.24 -0.3 2,389,828 - RR42_RS11190 0.24 +0.7 2,389,909 + RR42_RS11190 0.29 -0.5 2,389,910 - RR42_RS11190 0.29 +0.2 2,390,051 - RR42_RS11190 0.37 -0.3 2,390,210 + RR42_RS11190 0.47 +1.2 2,390,211 - RR42_RS11190 0.47 +0.1 2,390,211 - RR42_RS11190 0.47 -0.9
Or see this region's nucleotide sequence