Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11165 and RR42_RS11170 are separated by 70 nucleotides RR42_RS11170 and RR42_RS11175 are separated by 84 nucleotides RR42_RS11175 and RR42_RS11180 are separated by 4 nucleotides RR42_RS11180 and RR42_RS11185 are separated by 62 nucleotides
RR42_RS11165: RR42_RS11165 - methionine sulfoxide reductase B, at 2,386,335 to 2,386,781
_RS11165
RR42_RS11170: RR42_RS11170 - septation protein A, at 2,386,852 to 2,387,391
_RS11170
RR42_RS11175: RR42_RS11175 - BolA family transcriptional regulator, at 2,387,476 to 2,387,790
_RS11175
RR42_RS11180: RR42_RS11180 - peptidylprolyl isomerase, at 2,387,795 to 2,388,589
_RS11180
RR42_RS11185: RR42_RS11185 - TetR family transcriptional regulator, at 2,388,652 to 2,389,299
_RS11185
Position (kb)
2387
2388 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2386.539 kb on + strand, within RR42_RS11165 at 2386.540 kb on - strand, within RR42_RS11165 at 2387.389 kb on + strand at 2387.603 kb on - strand, within RR42_RS11175 at 2387.668 kb on + strand, within RR42_RS11175 at 2387.668 kb on + strand, within RR42_RS11175 at 2387.668 kb on + strand, within RR42_RS11175 at 2387.668 kb on + strand, within RR42_RS11175 at 2387.669 kb on - strand, within RR42_RS11175 at 2388.101 kb on + strand, within RR42_RS11180 at 2388.101 kb on + strand, within RR42_RS11180 at 2388.102 kb on - strand, within RR42_RS11180 at 2388.147 kb on - strand, within RR42_RS11180 at 2388.186 kb on - strand, within RR42_RS11180 at 2388.347 kb on + strand, within RR42_RS11180 at 2388.347 kb on + strand, within RR42_RS11180 at 2388.347 kb on + strand, within RR42_RS11180 at 2388.587 kb on + strand at 2388.587 kb on + strand at 2388.588 kb on - strand at 2388.588 kb on - strand at 2388.689 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,386,539 + RR42_RS11165 0.46 +0.2 2,386,540 - RR42_RS11165 0.46 +0.5 2,387,389 + -0.5 2,387,603 - RR42_RS11175 0.40 -0.3 2,387,668 + RR42_RS11175 0.61 -0.8 2,387,668 + RR42_RS11175 0.61 -0.5 2,387,668 + RR42_RS11175 0.61 -1.2 2,387,668 + RR42_RS11175 0.61 -0.1 2,387,669 - RR42_RS11175 0.61 +0.4 2,388,101 + RR42_RS11180 0.38 -3.7 2,388,101 + RR42_RS11180 0.38 -0.8 2,388,102 - RR42_RS11180 0.39 +0.2 2,388,147 - RR42_RS11180 0.44 -2.1 2,388,186 - RR42_RS11180 0.49 -1.1 2,388,347 + RR42_RS11180 0.69 -3.9 2,388,347 + RR42_RS11180 0.69 +2.2 2,388,347 + RR42_RS11180 0.69 -1.1 2,388,587 + -0.7 2,388,587 + -0.9 2,388,588 - +0.4 2,388,588 - -0.2 2,388,689 + -0.9
Or see this region's nucleotide sequence