Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11125 and RR42_RS11130 are separated by 74 nucleotides RR42_RS11130 and RR42_RS11135 overlap by 1 nucleotides RR42_RS11135 and RR42_RS11140 overlap by 4 nucleotides
RR42_RS11125: RR42_RS11125 - glycerophosphodiester phosphodiesterase, at 2,375,845 to 2,377,218
_RS11125
RR42_RS11130: RR42_RS11130 - mannosyltransferase, at 2,377,293 to 2,378,006
_RS11130
RR42_RS11135: RR42_RS11135 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,378,006 to 2,378,779
_RS11135
RR42_RS11140: RR42_RS11140 - ABC transporter permease, at 2,378,776 to 2,380,089
_RS11140
Position (kb)
2378
2379 Strain fitness (log2 ratio)
-2
-1
0
1 at 2377.216 kb on + strand at 2377.217 kb on - strand at 2377.294 kb on + strand at 2377.295 kb on - strand at 2377.472 kb on - strand, within RR42_RS11130 at 2377.611 kb on - strand, within RR42_RS11130 at 2377.645 kb on + strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.740 kb on - strand, within RR42_RS11130 at 2377.740 kb on - strand, within RR42_RS11130 at 2377.759 kb on + strand, within RR42_RS11130 at 2377.925 kb on + strand, within RR42_RS11130 at 2377.950 kb on - strand at 2377.967 kb on - strand at 2378.052 kb on + strand at 2378.331 kb on + strand, within RR42_RS11135 at 2378.332 kb on - strand, within RR42_RS11135 at 2378.356 kb on + strand, within RR42_RS11135 at 2378.357 kb on - strand, within RR42_RS11135 at 2378.445 kb on + strand, within RR42_RS11135 at 2378.446 kb on - strand, within RR42_RS11135 at 2378.512 kb on + strand, within RR42_RS11135 at 2378.512 kb on + strand, within RR42_RS11135 at 2378.513 kb on - strand, within RR42_RS11135 at 2378.654 kb on - strand, within RR42_RS11135 at 2378.654 kb on - strand, within RR42_RS11135 at 2378.750 kb on + strand at 2378.751 kb on - strand at 2378.751 kb on - strand at 2378.807 kb on - strand at 2378.972 kb on + strand, within RR42_RS11140 at 2379.038 kb on + strand, within RR42_RS11140 at 2379.155 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.524 kb on + strand, within RR42_RS11140 at 2379.527 kb on + strand, within RR42_RS11140 at 2379.528 kb on - strand, within RR42_RS11140 at 2379.594 kb on + strand, within RR42_RS11140 at 2379.708 kb on - strand, within RR42_RS11140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,377,216 + -0.1 2,377,217 - -0.4 2,377,294 + -0.3 2,377,295 - -0.5 2,377,472 - RR42_RS11130 0.25 -1.1 2,377,611 - RR42_RS11130 0.45 -0.0 2,377,645 + RR42_RS11130 0.49 -0.7 2,377,646 - RR42_RS11130 0.49 -0.4 2,377,646 - RR42_RS11130 0.49 -0.5 2,377,646 - RR42_RS11130 0.49 +0.1 2,377,646 - RR42_RS11130 0.49 -0.7 2,377,646 - RR42_RS11130 0.49 -0.0 2,377,646 - RR42_RS11130 0.49 -1.3 2,377,646 - RR42_RS11130 0.49 -0.7 2,377,740 - RR42_RS11130 0.63 -0.8 2,377,740 - RR42_RS11130 0.63 +0.7 2,377,759 + RR42_RS11130 0.65 +0.8 2,377,925 + RR42_RS11130 0.89 +0.6 2,377,950 - -2.1 2,377,967 - -0.3 2,378,052 + +0.5 2,378,331 + RR42_RS11135 0.42 -0.7 2,378,332 - RR42_RS11135 0.42 +0.6 2,378,356 + RR42_RS11135 0.45 +0.5 2,378,357 - RR42_RS11135 0.45 -0.1 2,378,445 + RR42_RS11135 0.57 -0.8 2,378,446 - RR42_RS11135 0.57 +0.5 2,378,512 + RR42_RS11135 0.65 -0.8 2,378,512 + RR42_RS11135 0.65 -0.8 2,378,513 - RR42_RS11135 0.66 +0.0 2,378,654 - RR42_RS11135 0.84 +0.0 2,378,654 - RR42_RS11135 0.84 -0.5 2,378,750 + -0.8 2,378,751 - -0.5 2,378,751 - -1.2 2,378,807 - -0.6 2,378,972 + RR42_RS11140 0.15 -0.8 2,379,038 + RR42_RS11140 0.20 -1.3 2,379,155 + RR42_RS11140 0.29 +0.3 2,379,482 + RR42_RS11140 0.54 +0.3 2,379,482 + RR42_RS11140 0.54 +1.0 2,379,482 + RR42_RS11140 0.54 -0.9 2,379,482 + RR42_RS11140 0.54 +0.9 2,379,483 - RR42_RS11140 0.54 -0.3 2,379,483 - RR42_RS11140 0.54 -0.2 2,379,483 - RR42_RS11140 0.54 -1.3 2,379,524 + RR42_RS11140 0.57 -0.5 2,379,527 + RR42_RS11140 0.57 -0.7 2,379,528 - RR42_RS11140 0.57 -0.5 2,379,594 + RR42_RS11140 0.62 -0.9 2,379,708 - RR42_RS11140 0.71 +0.1
Or see this region's nucleotide sequence