Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11110 and RR42_RS11115 are separated by 28 nucleotides RR42_RS11115 and RR42_RS11120 overlap by 11 nucleotides RR42_RS11120 and RR42_RS11125 are separated by 230 nucleotides
RR42_RS11110: RR42_RS11110 - serine dehydratase, at 2,373,021 to 2,373,983
_RS11110
RR42_RS11115: RR42_RS11115 - ornithine cyclodeaminase, at 2,374,012 to 2,374,983
_RS11115
RR42_RS11120: RR42_RS11120 - DNA-binding protein, at 2,374,973 to 2,375,614
_RS11120
RR42_RS11125: RR42_RS11125 - glycerophosphodiester phosphodiesterase, at 2,375,845 to 2,377,218
_RS11125
Position (kb)
2374
2375 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2373.259 kb on + strand, within RR42_RS11110 at 2373.259 kb on + strand, within RR42_RS11110 at 2373.260 kb on - strand, within RR42_RS11110 at 2373.260 kb on - strand, within RR42_RS11110 at 2373.314 kb on + strand, within RR42_RS11110 at 2373.315 kb on - strand, within RR42_RS11110 at 2373.379 kb on + strand, within RR42_RS11110 at 2373.380 kb on - strand, within RR42_RS11110 at 2373.539 kb on - strand, within RR42_RS11110 at 2373.605 kb on + strand, within RR42_RS11110 at 2373.616 kb on + strand, within RR42_RS11110 at 2373.616 kb on + strand, within RR42_RS11110 at 2373.617 kb on - strand, within RR42_RS11110 at 2373.617 kb on - strand, within RR42_RS11110 at 2373.617 kb on - strand, within RR42_RS11110 at 2373.755 kb on - strand, within RR42_RS11110 at 2373.759 kb on + strand, within RR42_RS11110 at 2373.759 kb on + strand, within RR42_RS11110 at 2373.760 kb on - strand, within RR42_RS11110 at 2373.925 kb on + strand at 2373.972 kb on + strand at 2374.387 kb on + strand, within RR42_RS11115 at 2374.388 kb on - strand, within RR42_RS11115 at 2374.388 kb on - strand, within RR42_RS11115 at 2374.388 kb on - strand, within RR42_RS11115 at 2374.497 kb on - strand, within RR42_RS11115 at 2374.497 kb on - strand, within RR42_RS11115 at 2374.879 kb on - strand, within RR42_RS11115 at 2374.879 kb on - strand, within RR42_RS11115 at 2374.983 kb on + strand at 2374.984 kb on - strand at 2374.987 kb on + strand at 2374.987 kb on + strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.989 kb on + strand at 2374.989 kb on + strand at 2374.990 kb on - strand at 2375.293 kb on - strand, within RR42_RS11120 at 2375.364 kb on + strand, within RR42_RS11120 at 2375.365 kb on - strand, within RR42_RS11120 at 2375.386 kb on - strand, within RR42_RS11120 at 2375.603 kb on - strand at 2375.603 kb on - strand at 2375.704 kb on + strand at 2375.705 kb on - strand at 2375.705 kb on - strand at 2375.705 kb on - strand at 2375.843 kb on - strand at 2375.843 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 2,373,259 + RR42_RS11110 0.25 -0.3 2,373,259 + RR42_RS11110 0.25 +1.6 2,373,260 - RR42_RS11110 0.25 +0.4 2,373,260 - RR42_RS11110 0.25 -0.8 2,373,314 + RR42_RS11110 0.30 -0.2 2,373,315 - RR42_RS11110 0.31 -1.8 2,373,379 + RR42_RS11110 0.37 -0.3 2,373,380 - RR42_RS11110 0.37 -1.3 2,373,539 - RR42_RS11110 0.54 -1.4 2,373,605 + RR42_RS11110 0.61 -0.3 2,373,616 + RR42_RS11110 0.62 -0.9 2,373,616 + RR42_RS11110 0.62 -0.3 2,373,617 - RR42_RS11110 0.62 +0.3 2,373,617 - RR42_RS11110 0.62 -0.6 2,373,617 - RR42_RS11110 0.62 +0.3 2,373,755 - RR42_RS11110 0.76 -0.1 2,373,759 + RR42_RS11110 0.77 +0.3 2,373,759 + RR42_RS11110 0.77 -0.9 2,373,760 - RR42_RS11110 0.77 +0.3 2,373,925 + +1.5 2,373,972 + -0.1 2,374,387 + RR42_RS11115 0.39 +0.4 2,374,388 - RR42_RS11115 0.39 -0.3 2,374,388 - RR42_RS11115 0.39 -0.3 2,374,388 - RR42_RS11115 0.39 +0.0 2,374,497 - RR42_RS11115 0.50 -1.1 2,374,497 - RR42_RS11115 0.50 +1.0 2,374,879 - RR42_RS11115 0.89 -0.3 2,374,879 - RR42_RS11115 0.89 -0.9 2,374,983 + +0.1 2,374,984 - -0.3 2,374,987 + -1.3 2,374,987 + -0.8 2,374,988 - +1.8 2,374,988 - -0.3 2,374,988 - +0.1 2,374,988 - -1.1 2,374,988 - +0.7 2,374,989 + -0.7 2,374,989 + -2.7 2,374,990 - +0.3 2,375,293 - RR42_RS11120 0.50 -1.4 2,375,364 + RR42_RS11120 0.61 -0.4 2,375,365 - RR42_RS11120 0.61 -0.2 2,375,386 - RR42_RS11120 0.64 -1.9 2,375,603 - +0.0 2,375,603 - -0.4 2,375,704 + -0.6 2,375,705 - -0.3 2,375,705 - -0.3 2,375,705 - -0.6 2,375,843 - +3.0 2,375,843 - -2.2
Or see this region's nucleotide sequence