Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08915 and RR42_RS08920 are separated by 17 nucleotides RR42_RS08920 and RR42_RS08925 are separated by 11 nucleotides RR42_RS08925 and RR42_RS08930 overlap by 4 nucleotides
RR42_RS08915: RR42_RS08915 - nitrate reductase, at 1,873,166 to 1,876,969
_RS08915
RR42_RS08920: RR42_RS08920 - nitrate reductase, at 1,876,987 to 1,878,510
_RS08920
RR42_RS08925: RR42_RS08925 - nitrate reductase, at 1,878,522 to 1,879,202
_RS08925
RR42_RS08930: RR42_RS08930 - nitrate reductase, at 1,879,199 to 1,879,942
_RS08930
Position (kb)
1876
1877
1878
1879 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1876.309 kb on + strand, within RR42_RS08915 at 1876.413 kb on - strand, within RR42_RS08915 at 1876.532 kb on + strand, within RR42_RS08915 at 1876.533 kb on - strand, within RR42_RS08915 at 1876.533 kb on - strand, within RR42_RS08915 at 1876.625 kb on - strand at 1876.715 kb on + strand at 1876.716 kb on - strand at 1876.716 kb on - strand at 1876.783 kb on + strand at 1876.819 kb on + strand at 1876.842 kb on - strand at 1876.842 kb on - strand at 1876.847 kb on + strand at 1876.859 kb on + strand at 1876.860 kb on - strand at 1876.860 kb on - strand at 1877.046 kb on + strand at 1877.047 kb on - strand at 1877.047 kb on - strand at 1877.099 kb on - strand at 1877.099 kb on - strand at 1877.288 kb on - strand, within RR42_RS08920 at 1877.298 kb on + strand, within RR42_RS08920 at 1877.340 kb on + strand, within RR42_RS08920 at 1877.488 kb on + strand, within RR42_RS08920 at 1877.489 kb on - strand, within RR42_RS08920 at 1877.518 kb on + strand, within RR42_RS08920 at 1877.519 kb on - strand, within RR42_RS08920 at 1877.588 kb on - strand, within RR42_RS08920 at 1877.588 kb on - strand, within RR42_RS08920 at 1877.668 kb on + strand, within RR42_RS08920 at 1877.680 kb on + strand, within RR42_RS08920 at 1877.680 kb on + strand, within RR42_RS08920 at 1877.680 kb on + strand, within RR42_RS08920 at 1877.680 kb on + strand, within RR42_RS08920 at 1877.681 kb on - strand, within RR42_RS08920 at 1877.681 kb on - strand, within RR42_RS08920 at 1877.683 kb on + strand, within RR42_RS08920 at 1877.684 kb on - strand, within RR42_RS08920 at 1877.684 kb on - strand, within RR42_RS08920 at 1877.684 kb on - strand, within RR42_RS08920 at 1878.023 kb on - strand, within RR42_RS08920 at 1878.046 kb on + strand, within RR42_RS08920 at 1878.148 kb on + strand, within RR42_RS08920 at 1878.148 kb on + strand, within RR42_RS08920 at 1878.149 kb on - strand, within RR42_RS08920 at 1878.227 kb on - strand, within RR42_RS08920 at 1878.313 kb on + strand, within RR42_RS08920 at 1878.314 kb on - strand, within RR42_RS08920 at 1878.337 kb on + strand, within RR42_RS08920 at 1878.377 kb on - strand at 1878.377 kb on - strand at 1878.508 kb on + strand at 1878.508 kb on + strand at 1878.509 kb on - strand at 1878.702 kb on + strand, within RR42_RS08925 at 1878.720 kb on + strand, within RR42_RS08925 at 1878.721 kb on - strand, within RR42_RS08925 at 1878.828 kb on + strand, within RR42_RS08925 at 1878.829 kb on - strand, within RR42_RS08925 at 1878.873 kb on + strand, within RR42_RS08925 at 1878.873 kb on + strand, within RR42_RS08925 at 1879.154 kb on + strand at 1879.155 kb on - strand at 1879.236 kb on - strand at 1879.236 kb on - strand at 1879.241 kb on + strand at 1879.241 kb on + strand at 1879.241 kb on + strand at 1879.242 kb on - strand at 1879.257 kb on + strand at 1879.257 kb on + strand at 1879.298 kb on + strand, within RR42_RS08930 at 1879.345 kb on - strand, within RR42_RS08930
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,876,309 + RR42_RS08915 0.83 +1.2 1,876,413 - RR42_RS08915 0.85 +0.3 1,876,532 + RR42_RS08915 0.88 +0.3 1,876,533 - RR42_RS08915 0.89 +2.2 1,876,533 - RR42_RS08915 0.89 -2.1 1,876,625 - -0.5 1,876,715 + -1.0 1,876,716 - +0.4 1,876,716 - +0.9 1,876,783 + -1.5 1,876,819 + +0.6 1,876,842 - -0.7 1,876,842 - -0.2 1,876,847 + -0.6 1,876,859 + +0.8 1,876,860 - +0.3 1,876,860 - -0.5 1,877,046 + +0.9 1,877,047 - +0.8 1,877,047 - +0.5 1,877,099 - -0.2 1,877,099 - +0.1 1,877,288 - RR42_RS08920 0.20 +0.4 1,877,298 + RR42_RS08920 0.20 +0.6 1,877,340 + RR42_RS08920 0.23 +0.5 1,877,488 + RR42_RS08920 0.33 +0.2 1,877,489 - RR42_RS08920 0.33 -0.4 1,877,518 + RR42_RS08920 0.35 +0.3 1,877,519 - RR42_RS08920 0.35 -0.1 1,877,588 - RR42_RS08920 0.39 +0.3 1,877,588 - RR42_RS08920 0.39 +0.6 1,877,668 + RR42_RS08920 0.45 +0.3 1,877,680 + RR42_RS08920 0.45 -2.8 1,877,680 + RR42_RS08920 0.45 -1.2 1,877,680 + RR42_RS08920 0.45 +1.2 1,877,680 + RR42_RS08920 0.45 -0.4 1,877,681 - RR42_RS08920 0.46 -0.4 1,877,681 - RR42_RS08920 0.46 -1.2 1,877,683 + RR42_RS08920 0.46 +1.1 1,877,684 - RR42_RS08920 0.46 -0.4 1,877,684 - RR42_RS08920 0.46 -1.3 1,877,684 - RR42_RS08920 0.46 -0.0 1,878,023 - RR42_RS08920 0.68 +0.5 1,878,046 + RR42_RS08920 0.69 +0.1 1,878,148 + RR42_RS08920 0.76 +0.3 1,878,148 + RR42_RS08920 0.76 +1.1 1,878,149 - RR42_RS08920 0.76 +0.9 1,878,227 - RR42_RS08920 0.81 +0.0 1,878,313 + RR42_RS08920 0.87 +0.3 1,878,314 - RR42_RS08920 0.87 +1.2 1,878,337 + RR42_RS08920 0.89 +2.7 1,878,377 - -1.0 1,878,377 - -1.0 1,878,508 + +0.3 1,878,508 + +0.2 1,878,509 - +0.4 1,878,702 + RR42_RS08925 0.26 +0.5 1,878,720 + RR42_RS08925 0.29 +0.4 1,878,721 - RR42_RS08925 0.29 -0.1 1,878,828 + RR42_RS08925 0.45 -0.4 1,878,829 - RR42_RS08925 0.45 -1.0 1,878,873 + RR42_RS08925 0.52 -0.2 1,878,873 + RR42_RS08925 0.52 +0.2 1,879,154 + -0.1 1,879,155 - +0.5 1,879,236 - -1.5 1,879,236 - +0.3 1,879,241 + -0.2 1,879,241 + -0.1 1,879,241 + -1.4 1,879,242 - +0.6 1,879,257 + +0.9 1,879,257 + -1.9 1,879,298 + RR42_RS08930 0.13 +1.8 1,879,345 - RR42_RS08930 0.20 -0.8
Or see this region's nucleotide sequence