Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07235 and RR42_RS07240 are separated by 851 nucleotides RR42_RS07240 and RR42_RS07245 are separated by 188 nucleotides RR42_RS07245 and RR42_RS07250 are separated by 20 nucleotides RR42_RS07250 and RR42_RS07255 are separated by 75 nucleotides
RR42_RS07235: RR42_RS07235 - hypothetical protein, at 1,542,652 to 1,542,936
_RS07235
RR42_RS07240: RR42_RS07240 - hypothetical protein, at 1,543,788 to 1,544,042
_RS07240
RR42_RS07245: RR42_RS07245 - hypothetical protein, at 1,544,231 to 1,544,521
_RS07245
RR42_RS07250: RR42_RS07250 - hypothetical protein, at 1,544,542 to 1,544,784
_RS07250
RR42_RS07255: RR42_RS07255 - hypothetical protein, at 1,544,860 to 1,545,111
_RS07255
Position (kb)
1543
1544
1545 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1542.815 kb on + strand, within RR42_RS07235 at 1542.815 kb on + strand, within RR42_RS07235 at 1542.816 kb on - strand, within RR42_RS07235 at 1542.936 kb on + strand at 1542.936 kb on + strand at 1542.936 kb on + strand at 1542.936 kb on + strand at 1542.937 kb on - strand at 1542.937 kb on - strand at 1542.937 kb on - strand at 1542.937 kb on - strand at 1542.937 kb on - strand at 1542.937 kb on - strand at 1542.937 kb on - strand at 1542.937 kb on - strand at 1542.938 kb on + strand at 1542.938 kb on + strand at 1542.938 kb on + strand at 1542.938 kb on + strand at 1542.938 kb on + strand at 1542.938 kb on + strand at 1542.938 kb on + strand at 1542.939 kb on - strand at 1542.939 kb on - strand at 1542.939 kb on - strand at 1542.939 kb on - strand at 1542.939 kb on - strand at 1542.939 kb on - strand at 1542.939 kb on - strand at 1542.939 kb on - strand at 1542.939 kb on - strand at 1543.015 kb on + strand at 1543.213 kb on + strand at 1543.218 kb on + strand at 1543.249 kb on + strand at 1543.249 kb on + strand at 1543.283 kb on + strand at 1543.321 kb on + strand at 1543.339 kb on + strand at 1543.339 kb on + strand at 1543.340 kb on - strand at 1543.346 kb on - strand at 1543.353 kb on - strand at 1543.431 kb on + strand at 1543.661 kb on + strand at 1543.661 kb on + strand at 1543.943 kb on + strand, within RR42_RS07240 at 1543.944 kb on - strand, within RR42_RS07240 at 1544.041 kb on - strand at 1544.103 kb on - strand at 1544.214 kb on + strand at 1544.214 kb on + strand at 1544.219 kb on + strand at 1544.225 kb on + strand at 1544.226 kb on - strand at 1544.381 kb on - strand, within RR42_RS07245 at 1544.420 kb on - strand, within RR42_RS07245 at 1544.536 kb on + strand at 1544.783 kb on - strand at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.835 kb on - strand at 1544.835 kb on - strand at 1544.894 kb on - strand, within RR42_RS07255 at 1544.894 kb on - strand, within RR42_RS07255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,542,815 + RR42_RS07235 0.57 +0.2 1,542,815 + RR42_RS07235 0.57 -1.9 1,542,816 - RR42_RS07235 0.58 -1.4 1,542,936 + -0.1 1,542,936 + +0.1 1,542,936 + +0.3 1,542,936 + -1.0 1,542,937 - +0.4 1,542,937 - +0.1 1,542,937 - +0.5 1,542,937 - -1.9 1,542,937 - -1.1 1,542,937 - +2.0 1,542,937 - -0.9 1,542,937 - -1.2 1,542,938 + +0.0 1,542,938 + +1.3 1,542,938 + +0.8 1,542,938 + +1.0 1,542,938 + -1.8 1,542,938 + +0.4 1,542,938 + +0.1 1,542,939 - +0.8 1,542,939 - -1.2 1,542,939 - +0.9 1,542,939 - -2.0 1,542,939 - -0.9 1,542,939 - -0.5 1,542,939 - +1.0 1,542,939 - -0.7 1,542,939 - -0.6 1,543,015 + +0.7 1,543,213 + -0.3 1,543,218 + -0.4 1,543,249 + -1.1 1,543,249 + +0.2 1,543,283 + -0.3 1,543,321 + -0.2 1,543,339 + +0.7 1,543,339 + +0.3 1,543,340 - -0.5 1,543,346 - -0.3 1,543,353 - +1.3 1,543,431 + +0.3 1,543,661 + -0.3 1,543,661 + -0.5 1,543,943 + RR42_RS07240 0.61 -1.7 1,543,944 - RR42_RS07240 0.61 +1.3 1,544,041 - +1.3 1,544,103 - -0.5 1,544,214 + +2.4 1,544,214 + +0.3 1,544,219 + +0.5 1,544,225 + +0.1 1,544,226 - -1.3 1,544,381 - RR42_RS07245 0.52 +1.0 1,544,420 - RR42_RS07245 0.65 +0.3 1,544,536 + -1.9 1,544,783 - -0.1 1,544,834 + +0.3 1,544,834 + +2.3 1,544,834 + -0.7 1,544,834 + +0.5 1,544,834 + +0.3 1,544,835 - -1.0 1,544,835 - -0.8 1,544,894 - RR42_RS07255 0.13 -0.7 1,544,894 - RR42_RS07255 0.13 -0.0
Or see this region's nucleotide sequence