Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06515 and RR42_RS06520 are separated by 344 nucleotides RR42_RS06520 and RR42_RS06525 are separated by 367 nucleotides RR42_RS06525 and RR42_RS06530 overlap by 4 nucleotides
RR42_RS06515: RR42_RS06515 - electron transfer flavoprotein-ubiquinone oxidoreductase, at 1,425,258 to 1,426,943
_RS06515
RR42_RS06520: RR42_RS06520 - short-chain dehydrogenase, at 1,427,288 to 1,428,073
_RS06520
RR42_RS06525: RR42_RS06525 - C4-dicarboxylate ABC transporter permease, at 1,428,441 to 1,429,049
_RS06525
RR42_RS06530: RR42_RS06530 - C4-dicarboxylate ABC transporter, at 1,429,046 to 1,430,350
_RS06530
Position (kb)
1427
1428
1429 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1427.007 kb on - strand at 1427.115 kb on - strand at 1427.189 kb on + strand at 1427.190 kb on - strand at 1427.277 kb on + strand at 1427.288 kb on - strand at 1427.563 kb on + strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.638 kb on + strand, within RR42_RS06520 at 1427.792 kb on + strand, within RR42_RS06520 at 1427.792 kb on + strand, within RR42_RS06520 at 1427.793 kb on - strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520 at 1428.071 kb on + strand at 1428.176 kb on + strand at 1428.344 kb on - strand at 1428.344 kb on - strand at 1428.544 kb on + strand, within RR42_RS06525 at 1428.545 kb on - strand, within RR42_RS06525 at 1428.628 kb on - strand, within RR42_RS06525 at 1428.628 kb on - strand, within RR42_RS06525 at 1428.864 kb on + strand, within RR42_RS06525 at 1428.864 kb on + strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.865 kb on - strand, within RR42_RS06525 at 1428.913 kb on - strand, within RR42_RS06525 at 1428.967 kb on + strand, within RR42_RS06525
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,427,007 - -0.5 1,427,115 - +1.3 1,427,189 + -0.2 1,427,190 - -1.9 1,427,277 + -0.1 1,427,288 - +0.9 1,427,563 + RR42_RS06520 0.35 +0.2 1,427,564 - RR42_RS06520 0.35 -0.2 1,427,564 - RR42_RS06520 0.35 -0.3 1,427,564 - RR42_RS06520 0.35 -0.6 1,427,638 + RR42_RS06520 0.45 +0.7 1,427,792 + RR42_RS06520 0.64 -0.3 1,427,792 + RR42_RS06520 0.64 +0.8 1,427,793 - RR42_RS06520 0.64 +0.4 1,427,888 + RR42_RS06520 0.76 -1.3 1,427,888 + RR42_RS06520 0.76 +1.8 1,427,888 + RR42_RS06520 0.76 -0.4 1,428,071 + -1.5 1,428,176 + +0.7 1,428,344 - -0.6 1,428,344 - -2.4 1,428,544 + RR42_RS06525 0.17 +0.3 1,428,545 - RR42_RS06525 0.17 -0.3 1,428,628 - RR42_RS06525 0.31 -2.4 1,428,628 - RR42_RS06525 0.31 +0.2 1,428,864 + RR42_RS06525 0.69 +1.2 1,428,864 + RR42_RS06525 0.69 +0.8 1,428,865 - RR42_RS06525 0.70 +0.5 1,428,865 - RR42_RS06525 0.70 +0.4 1,428,865 - RR42_RS06525 0.70 -2.8 1,428,865 - RR42_RS06525 0.70 -0.8 1,428,913 - RR42_RS06525 0.78 -0.4 1,428,967 + RR42_RS06525 0.86 +1.1
Or see this region's nucleotide sequence